Skip to main content
SpringerLink
Log in

Factors affecting callus and shoot formation from in vitro cultures of Lens culinaris Medik.

  • Short Communication
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

The influence of culture medium and explant on callus and shoot formation of lentil (Lens culinaris Medik.) has been studied. Three different explants (shoot-tip, first node and first pair of leaves) from three Spanish lentil cultivars were cultivated on two basal media: Murashige and Skoog medium (MS) and medium with mineral salts of MS medium plus vitamins of Gamborg's B5 medium (MSB), supplemented with growth regulators. Media with 2,4-D induced the formation of calli in all explants, but no organ regeneration was obtained from these calli. Multiple shoot formation was obtained from 33% to 92% of the explants in media supplemented with 2.25 mg l−1 of BA and 0.186 mg l−1 NAA+2.25 mg l−1 BA; in the other media one to two shoots per explant were formed in 10 to 98% of the explants. Root formation from explants was achieved only in media with NAA or IAA. Of the explants tested, the best morphogenetic responses were obtained from nodes and the poorest from leaves.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Mroginski, LA, Kartha, KK (1984) Tissue culture of legumes for crop improvement. In: Janick, J (Ed) Plant Breeding Rev, Vol 2 (pp 215–264). The AVI Publishing Company Inc, Westport, Conn

    Google Scholar 

  2. Kartha, KK, Pahl, NL, Leung, NL, Mroginski, LA (1981) Plant regeneration from meristems of grain legumes; soybean, cowpea, peanut, chickpea, and bean. Can J Bot 59: 1671–1679

    Google Scholar 

  3. Mroginski, LA, Kartha, KK, Shyluk, JP (1981) Regeneration of peanut (Arachis hypogaea) plantlets by in vitro culture of immature leaves. Can J Bot 59: 826–830

    Google Scholar 

  4. Rubluo, A, Kartha, KK, Mroginski, LA, Dyck, J (1984) Plant regeneration from pea leaflets cultured in vitro and genetic stability of regenerants. J Plant Physiol 117: 119–130

    Google Scholar 

  5. Allavena, A, Rossetti, L (1986) Micropropagation of bean (Phaseolus vulgaris L.); effect of genetic, epigenetic and environmental factors. Sci Hortic 30: 37–46

    Google Scholar 

  6. Barwale, UB, MeyerJr., MM, Widholm, JM (1986) Screening of Glycine max and Glycine soja genotypes for multiple shoot formation at the cotyledonary node. Theor Appl Genet 72: 423–428

    Google Scholar 

  7. Busse-Eisenreich, G (1986) In vitro cultivation of Vicia faba and induction of morphogenesis. Biol Zentralbl 105: 97–104

    Google Scholar 

  8. Wright, MS, Williams, MH, Pierson, PE, Carnes, MG (1987) Initiation and propagation of Glycine max L. Merr.: plants from tissue-cultured epicotyls. Plant Cell Tiss Org Cult 8: 83–90

    Google Scholar 

  9. Bajaj, YPS, Dhanju, MS (1979) Regeneration of plants from apical meristem tips of some legumes. Curr Sci 48: 906–907

    Google Scholar 

  10. Williams, DJ, McHughen, A (1986) Plant regeneration of the legume Lens culinaris Medik. (lentil) in vitro. Plant Cell Tiss Org Cult 7: 149–153

    Google Scholar 

  11. Saxena, PK, King, J (1987) Morphogenesis in lentil: Plant regeneration from callus cultures on Lens culinaris Medik. via somatic embryogenesis. Plant Sci 52: 223–227.

    Google Scholar 

  12. Murashige, T, Skoog, F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Google Scholar 

  13. Gamborg, OL, Miller, RA, Ojima, K (1986) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158

    Google Scholar 

  14. Mok, MC, Mok, DWS (1977) Genotypic responses to auxins in tissue cultures of Phaseolus. Physiol Plant 40: 261–264

    Google Scholar 

  15. Ruiz, ML, Peláez, MI, Rueda, J, Espino, FJ, Vázquez, AM (1986) A comparative study of callus formation and plant regeneration from different explants of Phaseolus vulgaris and Ph. coccineus. In: Horn, W, Jensen, CJ, Oderbach, W, Schieder, O (Eds) Genetic manipulation in plant breeding (pp 495–497). Walter de Gruyter, Berlin

    Google Scholar 

  16. Phillips, GC, Collins, GB (1979) In vitro tissue culture of selected legumes and plant regeneration from callus cultures of red clover. Crop Sci 19: 59–6

    Google Scholar 

  17. Narasimhulu, SB, Reddy, GM (1983) Plantlet regeneration from different callus cultures of Arachis hypogaea L. Plant Sci Lett 31: 157–163

    Google Scholar 

  18. Mroginski, LA, Kartha, KK (1981) Regeneration of plants from callus tissue of the forage legume Stylosanthes guianensis. Plant Sci Lett 23: 245–251

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Genética, Facultad de Biologia, Universidad de León, E-24071, León, Spain

    M. Carmen Polanco, M. Isabel Peláez & M. Luisa Ruiz

Authors
  1. M. Carmen Polanco
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Isabel Peláez
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Luisa Ruiz
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Polanco, M.C., Peláez, M.I. & Ruiz, M.L. Factors affecting callus and shoot formation from in vitro cultures of Lens culinaris Medik.. Plant Cell Tiss Organ Cult 15, 175–182 (1988). https://doi.org/10.1007/BF00035759

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00035759

Key words

Search

Navigation