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Enhanced plant regeneration from microspore-derived embryos of Brassica napus by chilling, partial desiccation and age selection

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Abstract

Germination was readily induced in recalcitrant microspore-derived embryos of Brassica napus ‘Topas’ when they were exposed to a period of chilling (9–12 days at 4°C) or partial desiccation (rapid or slow air drying) prior to germination. In general, embryos thirty-five days old had the highest germination rates as compared to younger or older ones. Populations of embryos were induced to germinate at a rate of over 90% under specific temperature, desiccation and age conditions. Comparisons to an embryogenic B. napus winter line, F346, are made.

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Authors and Affiliations

  1. Department of Crop Science, University of Guelph, N1G 2W1, Guelph, Ontario, Canada

    L. S. Kott & W. D. Beversdorf

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  1. L. S. Kott
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  2. W. D. Beversdorf
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Kott, L.S., Beversdorf, W.D. Enhanced plant regeneration from microspore-derived embryos of Brassica napus by chilling, partial desiccation and age selection. Plant Cell Tiss Organ Cult 23, 187–192 (1990). https://doi.org/10.1007/BF00034430

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  • DOI: https://doi.org/10.1007/BF00034430

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