Abstract
Vitamin B12 (Cyanocobalamin) is one of the vitamins believed to be produced exclusively by microorganisms. Although soil is a rich source of vitamin B12, systematic study as to possible uptake of this vitamin by the plant roots is lacking. This study was undertaken to investigate, under water culture conditions, the uptake of [57Co]-cyanocobalamin by soybean (Glycine max (L.) Merr.). In the range of 10 to 3200 μmol L−1, uptake of vitamin B12 was a linear function of the vitamin concentration in the nutrient solution. Depending on the vitamin concentration, 12 to 34% of the total absorbed vitamin was transported to the plant shoots, with proportionally more vitamin B12 transported at higher vitamin concentrations. Aeration of the rooting medium with nitrogen gas significantly increased the total uptake and the percentage of vitamin transported to the shoots. Addition of respiration inhibitor dinitrophenol to the nutrient solution did not affect the total uptake or the partitioning of the vitamin. Root temperature (5–30°C) did not affect the total uptake but significantly altered the partitioning of the vitamin between the roots and the shoots. Foliar-applied vitamin B12 was not translocated to any considerable degree to other plant parts, indicating that phloem transport does not contribute to the distribution of this vitamin within the plant. It is suggested that adding manure (which is rich in this vitamin) to the soil could increase soil and thus plant content of vitamin B12. This could be of importance in raising the intake of this vitamin by people living by choice or necessity on vegetarian diets who are usually threatened by vitamin B12 deficiency.
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References
Ahmed S and Evans H J 1961 The essentiality of cobalt for soybean plants grown under symbiotic conditions. Proc. Natl. Acad. Sci. (USA) 47, 24–36.
Barker A V 1975 Organic vs. inorganic nutrition and horticultural crop quality. HortScience 10, 50–53.
Beesley R C 1984 Uptake of vitamin B12 by isolated ileal entrocytes. J. Nutr. Sci. Vitaminol. 30, 209–220.
Chanarin I 1969 The Megaloblastic Anaemias. Blackwell Science Publishers. Oxford. 150 p.
Chen Y and Avnimelech Y (Eds) 1986 The Role of Organic Matter in Modern Agriculture. Martinus Nijhoff/Dr W. Junk Publishers. Dordrecht, The Netherlands.
Friedrich W 1988 Vitamins. Walter de Gruyter, Berlin. 1058 p.
Fries L 1962 Vitamin B12 in Pisum sativum (L.). Physiol. Plant. 15, 566–571.
Gray L F and Daniel L J 1959 Studies of vitamin B12 in turnip greens. J. Nutr. 67, 623–634.
Horn M A, Heinstein P F and Low P S 1990 Biotin-mediated delivery of exogenous macromolecules into soybean cells. Plant Physiol. 93, 1492–1496.
Jathar V S, Deshpande L V, Kulkarni P R, Satoskar R S and Rege D V 1974 Vitamin B 12-like activity in leafy vegetables. Indian J. Biochem. Biophys. 11, 71–73.
Kononova M M 1966 Soil Organic Matter. Pergamon Press, Oxford. 215 p.
Lehninger A L 1977 Biochemistry, 2nd ed. Worth, New York. 349 p.
Lewis N M, Kies C and Fox H M 1986 Vitamin B12 status of lacto-ovo-vegetarian and omnivore subjects fed controlled lacto-vegetarian, vegan and omnivore diets. Nutr. Rep. Int. 34, 197–206.
Lochhead A G and Thexton R H 1951 Vitamin B12 as a growth factor for soil bacteria. Nature 167, 1034.
Long A G and Wokes F 1968 Vitamins and minerals in plants. Plant Foods Hum. Nutr. 1, 43–50.
Machlin L J (Ed.) 1984 Handbook of Vitamins. Marcel Dekker New York. 512 p.
Maynard L A and Loosli J K 1969 Animal nutrition, 6th. Ed. pp 229–311. McGraw Hill, New York.
McFarlane C, Nolt C, Wicklife C, Pfleeger T, Shimabuku R and McDowell M 1987 The uptake, distribution and metabolism of four organic chemicals by soybean plants and barley roots. Environ. Toxicol. Chem. 6, 847–856.
Mozafar A and Oertli J J 1987 Effect of temperature on ion efflux properties of leaf cell compartments in several chilling-sensitive and chilling-tolerant plants. Can. J. Bot. 65, 1893–1900.
Oesterdahl B G, Janné K, Johansson E and Johansson H 1986 Determination of vitamin B12 in gruel by a radioisotope dilution assay. Inter J. Vit. Nutr. Res. 56, 95–99.
Paterson S, Mackay D, Tam D and Shiu W Y 1990 Uptake of organic chemicals by plants: A review of processes, correlations and models. Chemosphere 21, 297–331.
Pratt J J and Woldring M G 1982 Radioassay of vitamin B12 and other corrinoids. Methods Enzymol. 84, 369–406.
Pratt J M 1972 Inorganic Chemistry of Vitamin B12. Academic Press, London. 35 p.
Reisenauer H M 1960 Cobalt in nitrogen fixation by a legume. Nature 186, 375–376.
Robbins J Hervey A and Stebbins M E 1950 Studies on Euglena and vitamin B12. Science 112, 455.
Sebrell W H Jr and Harris R S (Eds) 1968 The Vitamins. pp 119–260. Academic Press. New York.
Seetharam B and Alpers D H 1985 Cellular uptake of cobalamin. Nutr. Rev. 43, 97–102.
Smith E L 1960 Vitamin B12. pp 15–22. Methuen, London.
Spector R 1981 Megavitamin therapy and the central nervous system. In Vitamins in Human Biology and Medicine. Ed. M H Briggs. CRC Press, Boca Raton, FL. 143 p.
Vaughan D and Malcolm R E (Eds) 1985 Soil Organic Matter and Biological Activity. Martinus Nijhoff/Dr W. Junk, Dordrecht, The Netherlands. 469 p.
Ziegler H 1975 Nature of transported substances. Encycl. Plant Physiol. New Series 1, 59–100.
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Mozafar, A., Oertli, J.J. Uptake of a microbially-produced vitamin (B12) by soybean roots. Plant Soil 139, 23–30 (1992). https://doi.org/10.1007/BF00012838
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DOI: https://doi.org/10.1007/BF00012838