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Decreased concentration of hemoglobin, accumulation of lipid oxidation products and unchanged skeletal muscle in Atlantic salmon (Salmo salar) fed low dietary vitamin E

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Abstract

Atlantic salmon (Salmo salar) fry, initial weight 0.16 g, were fed a semipurified diet with 0, 15, 30, 60 or 120 mg dl-α-tocopheryl acetate/kg. After 24 weeks, the first two of these groups were extinct, and the fish receiving 30 mg/kg were clearly vitamin E deficient. Vitamin E deficient fish had low hemoglobin levels, characterized by a combination of reduced cellular hemoglobin concentration, red cell volume and red cell number, and an increased number and fraction of immature red blood cells. The hemoglobin concentration decreased over the decreasing range of experimental dl-ga-tocopheryl acetate levels. Therefore, even if 60 mg dl-α-tocopheryl acetate/kg gave good survival, this level was clearly physiologically suboptimal. Ceroid accumulated in the liver of fish fed 30 mg vitamin E/kg, and autofluorescent inclusions were found in the red blood cells of fish fed 30 and 60 mg vitamin E/kg. Degeneration of skeletal muscle was not observed in the present study.

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Authors and Affiliations

  1. Directorate of Fisheries, Institute of Nutrition, P.O. Box 1900, N-5024, Bergen, Norway

    Kristin Hamre, Mette Lorentzen & Øyvind Lie

  2. Ministry of Fisheries, Div. Aquaculture, Institute of Marine Research, P.O. Box 1870, N-5024, Bergen, Norway

    Brit Hjeltnes

  3. Zoological Institute, University of Bergen, Allegaten 41, 5007, Bergen

    Harald Kryvi

  4. Laboratory of Clinical Biochemistry, Haukeland Unviersity Hospital, -5021, Bergen, Norway

    Sverre Sandberg

Authors
  1. Kristin Hamre
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  2. Brit Hjeltnes
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  3. Harald Kryvi
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  4. Sverre Sandberg
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  5. Mette Lorentzen
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  6. Øyvind Lie
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Hamre, K., Hjeltnes, B., Kryvi, H. et al. Decreased concentration of hemoglobin, accumulation of lipid oxidation products and unchanged skeletal muscle in Atlantic salmon (Salmo salar) fed low dietary vitamin E. Fish Physiol Biochem 12, 421–429 (1994). https://doi.org/10.1007/BF00004306

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