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Characterization of nine polymorphic microsatellite loci for a threatened saproxylic beetle Rosalia alpina (Coleoptera: Cerambycidae)

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Abstract

Nine polymorphic microsatellite loci were developed and characterized for the Rosalia longicorn, an endangered icon of European saproxylic biodiversity. The detected number of alleles per locus ranged from 2 to 4, and the observed and expected heterozygosities varied from 0.044 to 0.622, and from 0.086 to 0.613, respectively. All loci were in Hardy–Weinberg equilibrium as well, as no evidence of linkage disequilibrium was found. Despite the low level of polymorphism, all loci described in this study will provide a useful tool in future genetic studies of the Rosalia alpina species.

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Acknowledgments

We would like to thank Matthew Sweney for language corrections and Beata Baranová for support. This study and its authors were supported by the Technology Agency of the Czech Republic (TA02021501), the Grant Agency of the University of South Bohemia (168/2013/P), and the project Biodiversity of forest ecosystems CZ.1.07/2.3.00/20.0064.

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Authors and Affiliations

  1. Faculty of Sciences, University of South Bohemia, Ceske Budejovice, Czech Republic

    L. Drag & J. Zima Jr.

  2. Institute of Entomology, Biology Centre, Czech Academy of Sciences, Ceske Budejovice, Czech Republic

    L. Drag, J. Zima Jr. & L. Cizek

Authors
  1. L. Drag
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  2. J. Zima Jr.
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  3. L. Cizek
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Correspondence to L. Drag.

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Drag, L., Zima, J. & Cizek, L. Characterization of nine polymorphic microsatellite loci for a threatened saproxylic beetle Rosalia alpina (Coleoptera: Cerambycidae). Conservation Genet Resour 5, 903–905 (2013). https://doi.org/10.1007/s12686-013-9929-1

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  • DOI: https://doi.org/10.1007/s12686-013-9929-1

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