Abstract
The brown smooth-hound shark, Mustelus henlei (Triakidae), is an endemic member of the eastern Pacific shark assemblage considered both commercially and recreationally important. Here, microsatellite loci for M. henlei discovered by next-generation sequencing (Roche 454 pyrosequencing) are described. All loci were polymorphic (3–10 alleles) with observed heterozygosities between 0.24 and 0.89 and expected heterozygosities between 0.23 and 0.86. These loci are the first to be characterized explicitly for M. henlei and should be useful for the investigation of population structure and gene flow in this species and for other members of the Triakidae.
This is a preview of subscription content, log in via an institution to check access.
References
Boomer JJ, Stow AJ (2010) Rapid isolation of the first set of polymorphic microsatellite loci from the Australian gummy shark, Mustelus antarticus and their utility across divergent shark taxa. Conserv Genet Resour 2:393–395
Boutin-Ganache I, Raposo M, Raymond M, Deschepper CF (2001) M13-tailed primers improve the reliability and usability of microsatellite analyses performed with two different allele sizing methods. Biotechniques 31:24–26
Chabot CL, Nigenda S (2011) Characterization of 13 microsatellite loci for the tope shark, Galeorhinus galeus, discovered with next-generation sequencing and their utility for eastern Pacific smooth-hound sharks(Mustelus). Conserv Genet Resour (in press)
Compagno LJV (1984) FAO species catalogue vol. 4: sharks of the world, an annotated and illustrated catalogue of shark species known to date: pt. 2: carcharhiniformes. FAO Fish Synop 4:251–655
Compagno LJV, Dando M, Fowler S (2005) Sharks of the world. Princeton University Press, Princeton
Ebert DA (2003) Sharks, rays, and chimaeras of California. University of California Press, Berkeley
Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92–94
Goudet J (2003) FSTAT version 2. 9. 4: a program to estimate and test population genetics parameters. Updated from Goudet [1995]. Available from http://www.unil.ch/izea/softwares/fstat.html
Pérez Jiménez JC, Nishizaki OS, Castillo Geniz JL (2005) A new Eastern North Pacific Smoothhound Shark (Genus Mustelus, Family Triakidae) from the Gulf of California. Copeia 834–845
Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Rousset F (2008) Genepop’007: a complete reimplementation of the Genepop software for Windows and Linux. Mol Ecol Resour 8:103–106
Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132:365–386
Van Oosterhout C, Hutchinson B, Wills D, Shipley P (2003) Microchecker, microsatellite data checking software. Mol Ecol Fish Genet Lab
Acknowledgments
I would like to thank Mike McCorkle (McCorkle Fishing Enterprises) and Dan Pondella and Jonathan Williams (Occidental College) for providing samples, John Pollinger for the preparation of the 454 library, and Robert K. Wayne for laboratory equipment and reagents. Funding was provided by the American Museum of Natural History (Lerner-Gray Marine Research Grant).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Chabot, C.L. Characterization of 11 microsatellite loci for the brown smooth-hound shark, Mustelus henlei (Triakidae), discovered with next-generation sequencing. Conservation Genet Resour 4, 23–25 (2012). https://doi.org/10.1007/s12686-011-9464-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-011-9464-x