Abstract
We evaluated the antioxidant properties of mulberry leaves extract (MLE) and flavonoids isolated from MLE. MLE was prepared by extraction with methanol. Flavonoids were analyzed by high-performance liquid chromatography. Oxidative hemolysis of normal human red blood cells (RBCs) was induced by the aqueous peroxyl radical [2,2′-Azobis (2-amidinopropane) dihydrochloride, AAPH]. MLE contained three flavonoids in the order quercetin (QC) > kaempferol (KF) > astragalin (AG). Oxidative hemolysis of RBCs induced by AAPH was suppressed by MLE, AG, KF, and QC in a time- and dose-dependent manner. MLE and these three flavonoids prevented the depletion of cystosolic antioxidant glutathione (GSH) in RBCs. AG had the greatest protective effect against AAPH-induced oxidative hemolysis and GSH depletion in RBCs.
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Asano, N., T. Yamashita, K. Yasuda, K. Ikeda, H. Kizu, Y. Kameda, A. Kato, R.J. Nash, H.S. Lee, and K.S. Ryu. 2001. Polyhydroxylated alkaloids isolated from mulberry trees (Morus alba L.) and silkworms (Bombyxmori L.). Journal of Agriculture and Food Chemistry 49: 4208–4213.
Asgary, S., G. Naderi, and N. Askari. 2005. Protective effect of flavonoids against red blood cell hemolysis by free radicals. Experimental and Clinical Cardiology 10: 88–90.
Chan, K., H. Ho, C. Peng, K. Lan, M. Lin, H. Chen, and C. Wang. 2010. Polyphenol-rich extract from mulberry leaf inhibits vascular smooth muscle cell proliferation involving upregulation of p53 and inhibition of cyclin-dependent kinase. Journal of Agriculture and Food Chemistry 58: 2536–2542.
Csiszar, A., A. Podlutsky, M.S. Wolin, G. Losonczy, P. Pacher, and Z. Ungvari. 2009. Oxidative stress andaccelerated vascular aging: implications for cigarette smoking. Frontiers in Bioscience 14: 3128–3144.
Dai, F., Q. Miao, B. Zhou, L. Yang, and Z.L. Liu. 2006. Protective effects of flavonols and their glycosides against free radical-induced oxidative hemolysis of red blood cells. Life Sciences 78: 488–2493.
Enkhmaa, B., K. Shiwaku, T. Katsube, K. Kitajima, E. Anuurad, M. Yamasaki, and Y. Yamane. 2005. Mulberry (Morus alba L.) leaves and their major flavonol quercetin 3-(6-malonylglucoside) attenuate atherosclerotic lesion development in LDL receptor-deficient mice. Journal of Nutrition 135: 729–734.
Hseu, Y.C., W.C. Chang, Y.T. Hseu, C.Y. Lee, Y.J. Yech, P.C. Chen, J.Y. Chen, and H.L. Yang. 2007. Protection of oxidative damage by aqueous extract from Antrodiacamphorata mycelia in normal human erythrocytes. Life Sciences 71: 469–482.
Jani, N., J. Ziogas, J.A. Angus, and C.E. Wright. 2012. Exogenous glutathione is essential in the testing of antioxidant capacity using radical-induced haemolysis. Journal of Pharmacological and Toxicological Methods 65: 142–146.
Jones, D.P. 2008. Radical-free biology of oxidative stress. American Journal of Physiology. Cell Physiology. 295: 849–868.
Kaefer, C.M., and J.A. Milner. 2008. The role of herbs and spices in cancer prevention. Journal of Nutritional Biochemistry 19: 347–361.
Katsube, T., N. Imawaka, Y. Kawano, Y. Yamazaki, K. Shiwaku, and Y. Yamane. 2006. Antioxidant flavonol glycosides in mulberry (Morus alba L.) leaves isolated based on LDL antioxidant activity. Food Chemistry 97: 25–31.
Katsube, T., M. Yamasaki, K. Shiwaku, T. Ishijima, I. Matsumoto, K. Abe, and Y. Yamasaki. 2010. Effect of flavonol glycoside in mulberry (Morus alba L.) leaf on glucose metabolism and oxidative stress in liver in diet-induced obese mice. Journal of the Science of Food and Agriculture 90: 2386–2392.
Khan, M.A., A.A. Rahman, S. Islam, P. Khandokhar, S. Parvin, M.B. Islam, M. Hossain, M. Rashid, G. Sadik, S. Nasrin, M.N. Mollah, and A. Alam. 2013. A comparative study on the antioxidant activity of methanolic extracts from different parts of Morus alba L (Moraceae). BMC Research Notes 6: 24.
Kim, G.N., and H.D. Jang. 2011. Flavonol content in the water extract of the mulberry (Morus alba L.) leaf and their antioxidant capacities. Journal of Food Science 76: 869–873.
Kotani, M., M. Matsumoto, A. Fujita, S. Higa, W. Wang, M. Suemura, T. Kishimoto, and T. Tanaka. 2000. Persimmon leaf extract and astragalin inhibit development of dermatitis and IgE elevation in NC/Nga mice. The Journal of Allergy and Clinical Immunology 106: 159–166.
Loeser, R.F. 2011. Aging and Osteoarthritis. Current Opinion in Rheumatology 23: 492–496.
Luangaram, S., U. Kukongviriyapan, P. Pakdeechote, V. Kukongviriyapan, and P. Pannangpetch. 2007. Protective effects of quercetin against phenyl hydrazine-induced vascular dysfunction and oxidative stress in rats. Food and Chemical Toxicology 45: 448–455.
Maritim, A.C., and R.A. Sanders. 2003. Diabetes, oxidative stress, and antioxidants: a review. Journal of Biochemical and Molecular Toxicology 17: 24–38.
Matsumoto, M., M. Kotani, A. Fujita, S. Higa, T. Kishimoto, M. Suemura, and T. Tanaka. 2002. Oral administration of persimmon leaf extract ameliorates skin symptoms and transepidermal water loss in atopic dermatitis model mice, NC/Nga. The British Journal of Dermatology 146: 221–227.
Paiva-Martins, F., J. Fernandes, S. Rocha, H. Nascimento, R. Vitorino, F. Amado, F. Borges, L. Belo, and A. Santos-Silva. 2009. Effects of olive oil polyphenols on erythrocyte oxidative damage. Molecular Nutrition & Food Research 53: 609–616.
Peng, L., Y. YaLing, P. GuoPin, Z. FanRong, and W. QianQian. 2011. Effect of astragalin on paraoxon– induced vascular endothelium dysfunction. The Journal Board of Plant Diseases and Pests 2: 73–76.
Ray, P.D., B.W. Huang, and Y. Tsuji. 2012. Reactive oxygen species (ROS) homeostasis and redox regulationincellular signaling. Cellular Signalling 24: 981–990.
Rizzo, A.M., P.A. Corsetto, G. Montorfano, S. Milani, S. Zava, S. Tavella, R. Cancedda, and B. Berra. 2012. Effects of long-term space flight on erythrocytes and oxidative stress of rodents. PLoS ONE 7: e32361.
Sharma, A., P.N. Bernatchez, and J.B. de Haan. 2012. Targeting endothelial dysfunction in vascular complications associated with diabetes. International Journal of Vascular Medicine. doi:10.1155/2012/750126.
Stevenson, D.E., and R.D. Hurst. 2007. Polyphenolic phytochemicals-just antioxidants or much more? Cellular and Molecular Life Sciences 64: 2900–2916.
Tang, W.H., K.A. Martin, and J. Hwa. 2012. Aldose reductase, oxidative stress, and diabetic mellitus. Frontiers in Pharmacology 3: 87.
Wang, K.J., C.R. Yang, and Y. Zhang. 2007. Phenolic antioxidants from Chinese toon (fresh young leaves and shoots of Toona sinensis). Food Chemistry 101: 365–371.
Xiao, Z.P., H.K. Wu, T. Wu, H. Shi, B. Hang, and H.A. Aisa. 2006. Kaempferol and quercetin flavonoids from Rosa rugosa. Chemistry of Natural Compounds 42(7): 36–737.
Zhishen, J., T. Mengcheng, and W. Jianming. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry 64: 555–559.
Zou, C.G., N.S. Agar, and G.L. Jones. 2001. Oxidative insult to human red blood cells induced by free radical initiator AAPH and its inhibition by a commercial antioxidant mixture. Life Sciences 169: 75–86.
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This work was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project No. 00966303)” Rural Development Administration, Republic of Korea.
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Choi, J., Kang, H.J., Kim, S.Z. et al. Antioxidant effect of astragalin isolated from the leaves of Morus alba L. against free radical-induced oxidative hemolysis of human red blood cells. Arch. Pharm. Res. 36, 912–917 (2013). https://doi.org/10.1007/s12272-013-0090-x
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DOI: https://doi.org/10.1007/s12272-013-0090-x