Skip to main content
SpringerLink
Log in

Clonality investigation of morphologically dysplastic hematopoietic cells in myelodysplastic syndrome marrows

  • Original Article
  • Published:
International Journal of Hematology Aims and scope Submit manuscript

Abstract

The aim of this paper was to investigate whether the morphological dysplastic cells in myelodysplastic syndromes (MDS) marrows derived from abnormal chromosomal clone. Fluorescence in situ hybridization was used in combination with immunochemistry/immuno-magnetic bead sorting to investigate the penetration of chromosomal abnormalities into dysplastic hematopoietic cells in MDS patients with documented chromosome abnormalities in the bone marrow at diagnosis. Typical granulocyte dysplasia was defined as granulocytes with bilobed pseudo Pelger–Huet anomaly, erythrocyte dysplasia as tri-nucleus or nuclear budding erythrocytes (21 patients were investigated, respectively) and megakaryocytes dysplasia as lymphocytic appearance megakaryocytes (14 patients were investigated). When data were analyzed as a whole, the mean percentage of clonal cells with typical dysplasis was always lower than clonal cell percentage in all nucleated cells no matter whether in granulocytes, erythrocytes or megakaryocytes. For each individual case, the situation was the same with just few exceptions. The chromosome ploidy of micro-megakaryocytes was obviously reduced when compared to reported normal megakaryocytes. Our research suggested that tri-lineage morphological dyshematopoiesis is secondum alteration not a specific feature (imagery) of the abnormal chromosomal clone in MDS.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Baurmann H, Schwarz TF, Oertel J. Acute parvovirus B19 infection mimicking myelodysplastic syndrome of the bone marrow. Ann Hematol. 1992;l64:43–5.

    Article  Google Scholar 

  2. Bennett JM, Catovsky D, Daniel MT, Flandrin G, Galton DA, Gralnick HR, Sultan C. The French–American–British (FAB) co-operative group, proposals for the classification of the myelodysplastic syndromes. Br J Haematol. 1982;51:189–99.

    Article  CAS  PubMed  Google Scholar 

  3. Bowen D. What is ineffective erythropoiesis in myelodysplastic syndromes? Leuk Lymphoma. 1995;18:243–7.

    Article  CAS  PubMed  Google Scholar 

  4. Cantu Rajnoldi A, Fenu S, Kerndrup G, van Wering ER, Niemeyer CM, Baumann I; European Working Group on Myelodysplastic Syndromes in Childhood (EWOG-MDS). Evaluation of dysplastic features in myelodysplastic syndromes: experience from the morphology group of the European Working Group of MDS in Childhood (EWOG-MDS). Ann Hematol 2005;84:429–33.

    Article  CAS  PubMed  Google Scholar 

  5. Fontenay-Roupie M, Bouscary D, Guesnu M, Picard F, Melle J, Lacombe C, Gisselbrecht S, Mayeux P, Dreyfus F. Ineffective erythropoiesis in myelodysplastic syndromes: correlation with Fas expression but not with lack of erythropoietin receptor signal transduction. Br J Haematol. 1999;106:464–73.

    Article  CAS  PubMed  Google Scholar 

  6. Gladwin AM, Martin JF. The control of megakaryocyte ploidy and platelet production: biology and pathology. Int J Cell Cloning. 1990;8:291–8.

    Article  CAS  PubMed  Google Scholar 

  7. Goasguen JE, Bennett JM. Classification and morphologic features of the myelodysplastic syndromes. Semin Oncol. 1992;19:4–13.

    CAS  PubMed  Google Scholar 

  8. Gregg XT, Reddy V, Prchal JT. Copper deficiency masquerading as myelodysplastic syndrome. Blood. 2002;100:1493–5.

    Article  CAS  PubMed  Google Scholar 

  9. Hast R, Eriksson M, Widell S, et al. Neutrophil dysplasia is not a specific feature of the abnormal chromosomal clone in myelodysplastic syndromes. Leuk Res. 1999;23:579–84.

    Article  CAS  PubMed  Google Scholar 

  10. Hast R, Nilsson I, Widell S, Ost A. Diagnostic significance of dysplastic features of peripheral blood polymorphs in myelodysplastic syndromes. Leuk Res. 1989;13:173–8.

    Article  CAS  PubMed  Google Scholar 

  11. Hast R, Widell S. Dysplastic peripheral blood polymorphs link acute myeloblastic leukemia in elderly to the myelodysplastic syndromes. Eur J Haematol. 1992;48:163–7.

    Article  CAS  PubMed  Google Scholar 

  12. Hellstroröm-Lindberg E, Kanthor-Lewensohn L, Öst Å. Morphological changes and apoptosis in bone marrow from patients with myelodysplastic syndromes treated with granulocyte-CSF and erythropoietin. Leuk Res. 1997;21:415–25.

    Article  Google Scholar 

  13. Irving JA, Mattman A, Lockitch G, Farrell K, Wadsworth LD. Element of caution: a case of reversible cytopenias associated with excessive zinc supplementation. CMAJ. 2003;169:129–31.

    PubMed  PubMed Central  Google Scholar 

  14. Kobayashi Y, Ozawa M, Maruo N, Kondo M. Megakaryocyte ploidy in patients with myelodysplastic syndrome—by microcytofluorometry with DAPI staining after removal of Wright-Giemsa staining. Rinsho Ketsueki. 1989;30:1915–22.

    CAS  PubMed  Google Scholar 

  15. Levine RF. Isolation and characterization of normal human megakaryocytes. Br J Haematol. 1980;45:487–97.

    Article  CAS  PubMed  Google Scholar 

  16. Li X, Bryant CE, Deeg HJ. Simultaneous demonstration of clonal chromosome abnormalities and apoptosis in individual marrow cells in myelodysplastic syndrome. Int J Hematol. 2004;80:140–5.

    Article  PubMed  Google Scholar 

  17. Li X, Pu Q. Megakaryocytopoiesis and apoptosis in patients with myelodysplastic syndromes. Leuk Lymphoma. 2005;46:387–91.

    Article  CAS  PubMed  Google Scholar 

  18. Multani AS, Hopwood VL, Pathak S. A modified fluorescence in situ hybridization (FISH) technique. Anticancer Res. 1996;16:3435–7.

    CAS  PubMed  Google Scholar 

  19. Ohshima K, Kikuchi M, Takeshita M. A megakaryocyte analysis of the bone marrow in patients with myelodysplastic syndrome, myeloproliferative disorder and allied disorders. J Pathol. 1995;177:181–9.

    Article  CAS  PubMed  Google Scholar 

  20. Pagliuca A, Mufti GJ. Clinicomorphological features of myelodysplastic syndromes. In: Mufti GJ, Galton DAG, editors. The myelodysplastic syndromes. Edinburgh: Churchill Livingstone; 1992.

    Google Scholar 

  21. Raza A, Gezer S, Mundle S, Gao XZ, Alvi S, Borok R, Rifkin S, Iftikhar A, Shetty V, Parcharidou A, Loew J, Marcus B, Khan Z, Chaney C, Showel J, Gregory S, Harvey Preisler H. Apoptosis in bone marrow biopsy samples involving stromal and hematopoietic cells in 50 patients with myelodysplastic syndromes. Blood. 1995;86:268–76.

    CAS  PubMed  Google Scholar 

  22. Rosati S, Anastasi J, Vardiman J. Recurring diagnostic problems in the pathology of the myelodysplastic syndromes. Semin Hematol. 1996;l33:111–26.

    Google Scholar 

  23. Sawanobori M, Yamaguchi S, Hasegawa M, Inoue M, Suzuki K, Kamiyama R, Hirokawa K, Kitagawa M. Expression of TNF receptors and related signaling molecules in the bone marrow from patients with myelodysplastic syndromes. Leuk Res. 2003;27:583–91.

    Article  CAS  PubMed  Google Scholar 

  24. Van Lom K, Houtsmuller AB, van Putten WL, Slater RM, Lowenberg B. Cytogenetic clonality analysis of megakaryocytes in myelodysplastic syndrome by dual-color fluorescence in situ hybridization and confocal laser scanning microscopy. Genes Chromosomes Cancer. 1999;25:332–8.

    Article  PubMed  Google Scholar 

  25. Westhoff DD, Samaha RJ, Barnes AJ. Arsenic intoxication as a cause of megaloblastic anemia. Blood. 1975;45:241–6.

    CAS  PubMed  Google Scholar 

  26. Widell S, Hast R. Balloon-like platelets in myelodysplastic syndromes—a feature of dysmegakaryopoiesis? Leuk Res. 1987;11:747–52.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgment

This work is supported by a grant from Shanghai Municipal Science and Technology Committee (064119635), PRC.

Author information

Authors and Affiliations

  1. Department of Hematology, Sixth Hospital Affiliated to Shanghai Jiaotong University, Shanghai, 200233, China

    Xiao Li, Lingyun Wu, Shaoxu Ying, Chunkang Chang & Quan Pu

Authors
  1. Xiao Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lingyun Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shaoxu Ying
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chunkang Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Quan Pu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xiao Li.

About this article

Cite this article

Li, X., Wu, L., Ying, S. et al. Clonality investigation of morphologically dysplastic hematopoietic cells in myelodysplastic syndrome marrows. Int J Hematol 87, 176–183 (2008). https://doi.org/10.1007/s12185-008-0038-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12185-008-0038-y

Keywords

Search

Navigation