Abstract
Jerusalem artichoke is a low-requirement sugar crop containing cellulose and hemicellulose in the stalk and a high content of inulin in the tuber. However, the lignocellulosic component in Jerusalem artichoke stalk reduces the fermentability of the whole plant for efficient bioethanol production. In this study, Jerusalem artichoke stalk was pretreated sequentially with dilute acid and alkali, and then hydrolyzed enzymatically. During enzymatic hydrolysis, approximately 88 % of the glucan and xylan were converted to glucose and xylose, respectively. Batch and fed-batch simultaneous saccharification and fermentation of both pretreated stalk and tuber by Kluyveromyces marxianus CBS1555 were effectively performed, yielding 29.1 and 70.2 g/L ethanol, respectively. In fed-batch fermentation, ethanol productivity was 0.255 g ethanol per gram of dry Jerusalem artichoke biomass, or 0.361 g ethanol per gram of glucose, with a 0.924 g/L/h ethanol productivity. These results show that combining the tuber and the stalk hydrolysate is a useful strategy for whole biomass utilization in effective bioethanol fermentation from Jerusalem artichoke.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Margaritis, A., & Bajpai, P. (1982). Ethanol production from Jerusalem artichoke tubers (Helianthus tuberosus) using Kluyveromyces marxianus and Saccharomyces rosei. Biotechnology and Bioengineering, 24, 941–953.
Kosarik, M., Cosentino, G. P., & Weiczorek, A. (1984). The Jerusalem artichoke as an agricultural crop. Biomass, 5, 1–36.
Negro, M. J., Ballesteros, I., Manzanares, P., Oliva, J. M., Sáez, F., & Ballesteros, M. (2006). Inulin-containing biomass for ethanol production: carbohydrate extraction and ethanol fermentation. Applied Biochemistry and Biotechnology, 129–132, 922–932.
Bajpai, P., & Margaritis, A. (1982). Ethanol inhibition kinetics of Kluyveromyces marxianus grown on Jerusalem artichoke juice. Applied and Environmental Microbiology, 44, 1325–1329.
Margaritis, A., & Bajpai, P. (1982). Continuous ethanol production from Jerusalem artichoke tubers. I. Use of free cells of Kluyveromyces marxianus. Biotechnology and Bioengineering, 24, 1473–1482.
Kim, K., & Hamdy, M. K. (1986). Acid hydrolysis of Jerusalem artichoke for ethanol fermentation. Biotechnology and Bioengineering, 28, 138–141.
Favela-Torres, E., Allais, J. J., & Baratti, J. (1986). Kinetics of batch fermentations for ethanol production with Zymomonas mobilis growing on Jerusalem artichoke juice. Biotechnology and Bioengineering, 28, 850–856.
Rosa, M. F., Correia, I. S., & Novais, J. M. (1988). Improvements in ethanol tolerance of Kluyveromyces fragilis in Jerusalem artichoke juice. Biotechnology and Bioengineering, 31, 705–710.
Szambelan, K., Nowak, J., & Czarnecki, Z. (2004). Use of Zymomonas mobilis and Saccharomyces cerevisiae mixed with Kluyveromyces fragilis for improved ethanol production from Jerusalem artichoke tubers. Biotechnology Letters, 26, 845–848.
Li, D., Dai, J. Y., & Xiu, Z. L. (2010). A novel strategy for integrated utilization of Jerusalem artichoke stalk and tuber for production of 2,3-butanediol by Klebsiella pneumonia. Bioresource Technology, 101, 8342–8347.
Lynd, L. R., van Zyl, W. H., McBride, J. E., & Laser, M. (2005). Consolidated bioprocessing of cellulosic biomass: an update. Current Opinion in Biotechnology, 16, 577–583.
Gnansounou, E., & Dauriat, A. (2010). Techno-economic analysis of lignocellulosic ethanol: a review. Bioresource Technology, 101, 4980–4991.
FitzPatrick, M., Champagne, P., Cunningham, M. F., & Whitney, R. A. (2010). A biorefinery processing perspective: treatment of lignocellulosic materials for the production of value-added products. Bioresource Technology, 101, 8915–8922.
da Costa Sousa, L., Chundawat, S. P., Balan, V., & Dale, B. E. (2009). ‘Cradle-to-grave’ assessment of existing lignocellulose pretreatment technologies. Current Opinion in Biotechnology, 20, 339–347.
Alvira, P., Tomás-Pejó, E., Ballesteros, M., & Negro, M. J. (2010). Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review. Bioresource Technology, 101, 4851–4861.
Kim, S., Park, J. M., Seo, J. W., & Kim, C. H. (2012). Sequential acid-/alkali-pretreatment of empty palm fruit bunch fiber. Bioresource Technology, 109, 229–233.
Acknowledgments
This research was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea to C.H. Kim, and by the New & Renewable Energy Technology Development Program (2011T100200066) of the Korea Institute of Energy Technology Evaluation and Panning (KETEP) grant by the Korea government Ministry of Knowledge Economy to S. Kim.
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Kim, S., Park, J.M. & Kim, C.H. Ethanol Production Using Whole Plant Biomass of Jerusalem Artichoke by Kluyveromyces marxianus CBS1555. Appl Biochem Biotechnol 169, 1531–1545 (2013). https://doi.org/10.1007/s12010-013-0094-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-013-0094-5