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Isolation and Biomass Production of a Saccharomyces cerevisiae Strain Binding Copper and Zinc Ions

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Abstract

Copper and zinc are essential trace elements participating in many physiological functions, notably immunity and protection against oxidative stress. Yeasts and the yeast Saccharomyces cerevisiae, in particular, possess in their genome tandem repeats of the CUP1 gene coding for a protein (a metallothionein) capable of capturing and binding toxic elements such as copper ions. The number of copies of this gene in a cell determines its physiological level of resistance to these ions. This paper describes the selection, characterization, and production of a new copper-resistant yeast strain that can bind large quantities of copper and zinc. This approach should lead to increasing the bioavailability of these trace elements and hence to reducing their emission into the environment.

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Acknowledgments

This work was supported by the “Ministère belge de la Région Wallonne” (contract RW-DGA-31–1111). We wish to thank Anne-Lise Boulvin for providing technical assistance.

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Authors and Affiliations

  1. Unité de Biologie animale et microbienne, FUSAGx, Avenue Maréchal Juin, 6, 5030, Gembloux, Belgique

    Aurore Stroobants, Jean-Marc Delroisse, Daniel Portetelle & Micheline Vandenbol

  2. Unité de Bio-industries, FUSAGx, Passage des Déportés, 2, 5030, Gembloux, Belgium

    Franck Delvigne

  3. Département “Sciences et Gestion de l’Environnement”, ULg, Avenue de Longwy, 185, 6700, Arlon, Belgium

    Julien Delva

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  1. Aurore Stroobants
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  2. Jean-Marc Delroisse
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  3. Franck Delvigne
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  4. Julien Delva
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  5. Daniel Portetelle
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  6. Micheline Vandenbol
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Correspondence to Aurore Stroobants.

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Stroobants, A., Delroisse, JM., Delvigne, F. et al. Isolation and Biomass Production of a Saccharomyces cerevisiae Strain Binding Copper and Zinc Ions. Appl Biochem Biotechnol 157, 85–97 (2009). https://doi.org/10.1007/s12010-008-8253-9

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  • DOI: https://doi.org/10.1007/s12010-008-8253-9

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