Effects of moxibustion pretreatment on GSH-Px, SOD and MDA in gastric mucosa of rats with stress ulcer

Abstract

Objective

To observe the effects of moxibustion pretreatment on Glutathione Peroxidase (GSH-Px), Superoxide Dismutase (SOD), and Malondialchehyche (MDA) in gastric mucosa of rats with stress ulcer, and to investigate the mechanism of moxibustion pretreatment preventing the oxidized injury of gastric mucosa.

Methods

Forty-eight healthy SD rats were randomly allocated into 4 groups, namely control group, model group, acupoint moxibustion group, and non-acupoint moxibustion group. The ulcer model was developed by restraint and water immersion stress, Ulcer index (UI) was calculated with Guth method, and the contents of GSH-Px, and MDA and SOD activity in the gastric tissues were measured with chromatometry method.

Results

Compared those in the model group and nonacupoint moxibustion group, moxibustion at Zusanli (ST 36) could decrease the UI (P<0.01) and MDA (P<0.05) contents, and increase the activity of GSH-Px (P<0.01) and SOD (P<0.05).

Conclusion

Pretreatment of moxibustion at Zusanli (ST 36) could promote the recovery of gastric injury in rats induced by restraint and water immersion stress by enhancing the activity of GSH-Px and SOD, and lower MDA content.

摘要

目的

观察艾灸预处理对应激性溃疡大鼠胃黏膜谷胱甘肽过氧化酶(Glutathione Peroxidase, GSH-Px)、 超氧化物歧化酶(Superoxide Dismutase, SOD)的活性与丙二醛(Malondialchehyche, MDA)的影响, 探讨艾灸预处理抗胃黏膜氧化损伤的作用机制。

方法

将48 只健康SD 大鼠随机分为4 组, 即空白组、 模型组、 艾灸穴位组、 艾灸非穴点对照组。 束缚冷应激法制作应激性溃疡模型, 按Guth法计算胃黏膜损伤指数(Ulcer Index, UI), 比色法测定胃组织GSH-Px、 SOD的活性与MDA的含量。

结果

与模型组和艾灸非穴点对照组比较, 艾灸足三里等穴可使应激性溃疡大鼠胃黏膜损伤指数明显下降(P<0.01)、 GSH-Px 活性增强(P<0.01)、 SOD活性增强(P<0.05)、 MDA含量降低(P<0.05)。

结论

艾灸足三里等穴预处理可促进束缚水浸应激所造成大鼠胃黏膜损伤的修复, 可能是通过增加GSH-Px与SOD活性、 降低MDA含量 而达到其抗氧化损伤作用。