Abstract
Objective
To investigate the effects of sodium tanshinone II A sulfonate (STS) on the hypertrophy induced by angiotensin II (Ang II) in primary cultured neonatal rat cardiac myocytes.
Methods
The effect of STS on cytotoxicity was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-3,5-phenytetrazoliumromide (MTT) assay. As indexes for cardiocyte hypertrophy, cell size was determined by phase contrast microscopy and protein synthesis rate was measured by 3H-leucine incorporation. The proto-oncogene c-fos mRNA expression of cardiocytes was assessed using reverse transcription polymerase chain reaction (RT-PCR).
Results
STS could inhibit cardiocyte hypertrophy, increase the protein synthesis rate and enhance proto-oncogene c-fos mRNA expression in cardiocytes induced by Ang II (P<0.01), with an effect similar to that of Valsartan, the Ang II receptor antagonist.
Conclusion
STS can prevent the hypertrophy of cardiac myocytes induced by Ang II, which may be related to its inhibition of the expression of proto-oncogene c-fos mRNA.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Messerli FH, Ketelhut R. Left ventricular hypertrophy: a pressure-independent cardiovascular risk factor. J Cardiovasc Pharmacol 1993; 22(suppl 1):s7–s13.
Sadoshima J, Izumo S. Molecular characterization of angiotensin II-induced hypertrophy of cardiac myocytes and hyperplasia of cardiac fibroblasts. Critical role of the AT1 receptor subtype. Cre Res 1993;73:413–423.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65:55–63.
Yang K, Mu XS, Xue JJ, et al. Increased expression of c-fos mRNA and AP-1 transcription factors after cortical impact injury in rat. Brain Res 1994;664:141–147.
Tang W, Eisenbrand G. Salvia militiorrhiza Bge. Chinese drugs of plant origin. Berlin: Spring-Verlag, Germany, 1992:891–902.
Shanghai Cooperative Group for the study of tanshinone II A. Therapeutic effect of tanshinone II A sulfonate in patients with coronary heart disease: a double blind study. J Tradit Chin Med 1984;4(1);20–24.
Hefti MA, Hander BA, Eppenberger HM, et al. Signaling of pathways in cardiac myocyte hypertrophy. J Mol Cell Cardiol 1997;29:2873–2892.
Sadoshima J, Izumo S. Signal transduction pathways of angiotensin II-induced c-fos gene expression in cardiac myocytes in vitro. Roles of phospholipid-derived second messengers. Circ Res 1993;73:424–438.
Ballard C, Schaffer S. Stimulation of the Na+/Ca2+ exchanger by phenylephrine, angiotensin II and endothelin 1. J Mol Cell Cardiol 1996;28:11–17.
Chien KR, Knowlton KU, Zhu H, et al. Regulation of cardiac gene expression during myocardial growth and hypertrophy: molecular studies of an adaptive physiologic response. FASEB J 1991; 5:3037–3046.
Liu QY, Tsai TD. Effects of sodium tanshinone II-A sulfonate on slow action potentials of dissociated single ventricular cells of adult guinea pig heart. Acta Physiol Sin 1990;42(3):254–261.
Li F, Wang XM, Li XZ, et al. A time-related study about morphometry in myocardial ultrastructure and Ca distribution in ischemia and reperfusion damage and the protective effect of Danshen for the heart. Chin J Pathophysiol (Chin) 1992; 8(6): 565–568.
Xu CQ, Fan JS, Hao XM, et al. Blocking effect of tanshinone II-A on L-type Ca2+ current of single ventricular myocyte from guinea pig. Chin J Pharmacol Toxical (Chin) 1996; 10(2): 81–84.
Author information
Authors and Affiliations
Corresponding author
Additional information
Supported by the National Natural Science Foundation of China (No. 30500657)
Rights and permissions
About this article
Cite this article
Feng, J., Zheng, Z. Effect of sodium tanshinone II A sulfonate on cardiac myocyte hypertrophy and its underlying mechanism. Chin. J. Integr. Med. 14, 197–201 (2008). https://doi.org/10.1007/s11655-008-0197-y
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11655-008-0197-y