Summary
To explore the correlation between the C807T polymorphism of platelet membrane glycoprotein I a (GP I a) gene and aspirin resistance in Chinese people, 200 patients with high-risk of atherosclerosis took aspirin (100 mg/d) for 7 days. Platelet aggregation function was detected using adenosine diphosphate (ADP) and arachidonic acid (AA) before and after the administration of aspirin. Then the subjects were divided into three groups according to the results of platelet aggregation function: an aspirin resistant (AR) group, an aspirin semi-responder (ASR) group and an aspirin-sensitive (AS) group. Platelet GP I a gene 807CT polymorphism was examined by means of polymerase chain reaction-sequence specific primers (PCR-SSP). The results showed that T allelic frequency in AR group and ASR group were higher that of AS group (P<0.005), and the prevalence of genotypes (TT+TC) of these two groups was significantly higher than that in AS group (P<0.05). Platelet GP I a T allele was significantly associated with aspirin resistance as revealed by multiple logistic regression (OR=3.76, 95% CI: 2.87–9.58). The results suggest that inherited platelet GP I a variations may have an important impact on aspirin resistance and the presence of GP I a T allele may be a marker of genetic susceptibility to aspirin resistance.
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References
Cambria-Kiely J A, Gandhi P J. Aspirin resistance and genetic polymorphisms. J Thromb Thrombolysis, 2002, 14(1):51–58
Gum P A, Kottke-Marchan K, Poggio E D et al. Profile and prevalence of aspirin resistance in patients with cardiovascular disease. Am J Cardiol, 2001,88(3):230–235
Santoso S, Kunicki T J, Kroll H et al. Association of the platelet glycoprotein I a C807T gene polymorphism with nonfatal myocardial infarction in younger patients. Blood, 1999,93(8):2449–2453
Saelman E U, Nieuwenhuis H K, Hese K M et al. Platelet adhesion to collagen types I through VIII under conditions of stasis and flow is mediated by GP I a/II a (alpha 2 beta 1-integrin). Blood, 1994,83(5):1244–1250
Kawasaki T, Ozeki Y, Igawa T et al. Increased platelet sensitivity to collagen in individuals resistant to low-dose aspirin. Stroke, 2000,31(3): 591–595
Kunicki T J, Kritzik M, Annis D S et al. Hereditary variation in platelet integrin alpha 2 beta 1 density is associated with two silent polymorphisms in the alpha 2 gene coding sequence. Blood, 1997, 89(6):1939–1943
Cambria-Kiely J A, Gandhi P J. Possible mechanisms of aspirin resistance. J Thromb Thrombolysis, 2002,13(1):49–56
Homoncik M, Jilma B, Hergovich N et al. Monitoring of aspirin (ASA) pharmacodynamics with the platelet function analyzer PFA-100. Thromb Haemost, 2000,83(2):316–321
Macchi L, Christiaens L, Brabant S et al. Resistance in vitro to low-dose aspirin is associated with platelet P1A1 (GPIIIa) polymorphism but not with C807T (GP I a/II a) and C-5T Kozak (GP I b alpha) polymorphisms. J Am Coll Cardiol, 2003,42(6):1115–1119
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Su, G., Wang, Z., Ding, Y. et al. Association of the platelet membrane glycoprotein I a C807T gene polymorphism with aspirin resistance. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 27, 664–667 (2007). https://doi.org/10.1007/s11596-007-0611-2
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DOI: https://doi.org/10.1007/s11596-007-0611-2