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Morphology, anatomy, and molecular studies of the ectomycorrhiza formed axenically by the fungus Sistotrema sp. (Basidiomycota)

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Abstract

Several species of the corticioid (resupinate) genus Sistotrema of the cantharelloid clade (Basidiomycota) were recently found to be ectomycorrhizal. This changed the traditional assertion that all Sistotrema species are strictly wood rotting and suggests that the genus may be polyphyletic. In the present investigation, a still unknown root tip-associated fungal specimen (EW63) was isolated and found to be associated with an above-ground fruiting body. Sequencing of the ITS and the nucLSU DNA regions and phylogenetic analyses verified that the root-associated fungus and the fruiting body represented the same species, which was found to belong to the genus Sistotrema. To prove the ectomycorrhizal status of this strain, axenic Pinus sylvestris resyntheses in flask cultures were conducted. Growth parameters of the seedlings were determined and the morphology and anatomy of the synthesized mycorrhizas were described. Length and dry mass of the Pinus shoot as well as those of the total root tips were found to be enhanced as a result of the mycorrhizal association. Mycorrhizal frequency was high (51.5%) in these cultures. Mycorrhizal root tips were cottony light ochre with a thin plectenchymatic hyphal mantle. The clamps of the fruiting body hyphae as well as the mycorrhiza were ampullately inflated. This is the first report proving in axenic culture that a fungus belonging to the genus Sistotrema forms true ectomycorrhiza.

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Acknowledgements

The authors are indebted to the German Research Foundation (DFG), project Mu 1035/9-3 for the financial support. We thank M. Roth for the excellent technical assistance and T. Juretzek and the Institute of Clinical Microbiology at the Carl Thiem Klinikum Cottbus for performing the automated sequencing. We also thank M.T. Lavin-Zimmer, GFZ Potsdam for English corrections.

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Authors and Affiliations

  1. Institute for Landscape Biogeochemistry, Leibniz‐Centre for Agricultural Landscape Research (ZALF), Eberswalder Straße 84, 15374, Müncheberg, Germany

    Babette Münzenberger & Ben Bubner

  2. Chair of Soil Protection and Recultivation, Brandenburg University of Technology, Box 101344, 03013, Cottbus, Germany

    Beate Schneider & Reinhard F. Hüttl

  3. Department of Plant and Environmental Sciences, Göteborg University, Box 461, 40530, Göteborg, Sweden

    R. Henrik Nilsson

  4. Natural History Museum, University of Oslo, Box 1172, 0318, Oslo, Norway

    Karl-Henrik Larsson

  5. German Research Centre of Geosciences Potsdam (GFZ), Telegrafenberg, 14473, Potsdam, Germany

    Reinhard F. Hüttl

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  1. Babette Münzenberger
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  2. Beate Schneider
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  3. R. Henrik Nilsson
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  4. Ben Bubner
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Correspondence to Babette Münzenberger.

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Münzenberger, B., Schneider, B., Nilsson, R.H. et al. Morphology, anatomy, and molecular studies of the ectomycorrhiza formed axenically by the fungus Sistotrema sp. (Basidiomycota). Mycol Progress 11, 817–826 (2012). https://doi.org/10.1007/s11557-011-0797-3

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