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Cognitive enhancement in aged mice after chronic administration of Cedrus deodara Loud. and Pinus roxburghii Sarg. with demonstrated antioxidant properties

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Abstract

Cedrus deodara and Pinus roxburghii, plants mentioned in Indian literature, have been described to possess central nervous system effects and are used in Ayurvedic medicine to treat disorders of the mind. To investigate the memory-enhancing activity of volatile oil and chloroform extracts of C. deodara and P. roxburghii in the Morris water maze paradigm as well as evaluating their antioxidant properties. Aged albino mice were used to study the effect of oil and chloroform extracts on learning and memory by using the Morris water maze paradigm. The chloroform extract of C. deodara produced a significant decrease in escape latency over 7 days of training in both reference and working memory training in comparison to the control group. In the probe trial on day 8, mice in the chloroform extract of C. deodara group crossed the target area more often and spent more time in the target quadrant. Amongst the oils and extracts tested for oxidative stress parameters, only the chloroform extract of C. deodara at doses of 100 mg/kg produced a significant decrease in malondialdehyde (MDA) with a simultaneous significant increase in the level of glutathione (GSH) in both the frontal cortex and hippocampus. The present findings indicate that the chloroform extract of C. deodara has the best memory-enhancing effect due to its strong antioxidant properties from compounds like terpenoids and flavonoids. The study provides a scientific rationale for the traditional use of C. deodara in the management of memory dysfunction and related disorders.

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References

  1. Cantuti CI, Shukitt-Hale B, Joseph JA (2000) Neurobehavioural aspects of antioxidants in aging. Int J Dev Neurosci 18(4–5):367–381

    Article  Google Scholar 

  2. Shah R (2006) Description of Pinus roxburghii Sarg. Nature’s Medicinal Plants of Uttaranchal. 1:18–19

    Google Scholar 

  3. Hussain M, Shah GM, Khan MA (2006) Traditional medicinal and economic uses of gymnosperms of Kaghan Valley, Pakistan. Ethnobot Leafl 10:72–81

    Google Scholar 

  4. Kunwar RM, Uprety Y, Burlakoti C, Chowdhary CL, Bussmann RW (2009) Indigenous use and ethnopharmacology of medicinal plants in Far-west Nepal. J Plant People Applied Res 7:005–028

    Google Scholar 

  5. Agarwal PK, Rastogi RP (1981) Terpenoids from Cedrus deodara. J Ethnopharmacol 20(6):1391–1421

    Google Scholar 

  6. Kirtikar KR, Basu BD (1991) Indian medical plants. In: Blaster E, Caius JF, Bhaskar KS (eds) Periodical Experts Book Agency, New Delhi

  7. Nadkarni KM, Nadkarni AK, editors (1996). Indian Materia Medica. Bombay Popular Prakashan

  8. Shinde UA, Phadke AS, Nair AM, Mungantiwar AA, Dikshit VJ, Saraf MN (1999) Membrane stabilizing activity: a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fitoterapia 70:251–257

    Article  CAS  Google Scholar 

  9. Saxena AK, Singh J, Bhushan S (2010) Natural antioxidants synergistically enhance the anticancer potential of AP9-cd, a novel lignan composition from Cedrus deodara in human leukemia HL-60 cells. Chemico-Biol Interact 188:580–590

    Article  CAS  Google Scholar 

  10. Viswanatha GL, Kumar KN, Shylaja H, Ramesh C, Rajesh S, Srinath R (2009) Anxiolytic and anticonvulsant activity of alcoholic extract of heart wood of Cedrus deodara Roxb. in rodents. J Pharm Res Health Care 1(2):217–239

    Google Scholar 

  11. Shivanand P, Viral D, Goyani M, Vaghani S, Jaganathan K (2009) Formulation and evaluation of Cedrus deodara Loud. extract. Int J Chem Tech Res 1(4):1145–1152

    CAS  Google Scholar 

  12. Shinde UA, Phadke AS, Nair AM, Mungantiwar AA, Dikshit VJ, Saraf MN (1999) Preliminary studies on the immunomodulatory activity of Cedrus deodara wood oil. Fitoterapia 70:333–339

    Article  CAS  Google Scholar 

  13. Singh SK, Shanmugavel M, Kampasi H, Singh R, Mondhe DM, Rao JM et al (2007) Chemically standardized isolates from Cedrus deodara stem wood having anticancer activity. Planta Med 73:519–526

    Article  CAS  PubMed  Google Scholar 

  14. Rao JM, Srinivas PV, Yadav JS, Raghavan KV, Saxena AK, Shanmugavel M, Kampasi H, Qazi GN (2003) Herbal chemical composition for the treatment of cancer. US Patent 6:649–650

    Google Scholar 

  15. Singh SK, Shanmugavel M, Kampasi H, Singh R, Mondhe DM, Rao JM, Adwankar MK, Saxena AK, Qazi GN (2007) Chemically standardized isolates from Cedrus deodara stem wood having anticancer activity. Planta Med 73:519–526

    Article  CAS  PubMed  Google Scholar 

  16. Shankaranarayan R, Krishnappa S, Bisarya SC, Dev S (1977) Studies in sesquiterpenes-LIII: deodarone and atlantolone, new sesquiterpenoids from the wood of Cedrus deodara Loud. Tetrahedron 33:1201–1205

    Article  CAS  Google Scholar 

  17. Agrawal PK, Agarwal SK, Rasgi RP (1980) Dihydroflavonoids from Cedrus deodara. Phytochemistry 19:893–896

    Article  CAS  Google Scholar 

  18. Chopra RN, Nayar SL, Chopra IC (1986) Glossary of Indian medicinal plants (including supplement). Council of Scientific and Industrial Research, New Delhi

    Google Scholar 

  19. Bajracharya MB (1979) Ayurvedic medicinal plants and general treatments. Jore Ganesh Press Pvt Ltd, Kathmandu

    Google Scholar 

  20. Dash VB, Gupta KK (1994) Materia medica of Ayurveda. B. Jain Publishers, New Delhi

    Google Scholar 

  21. Uniyal SK, Singh KN, Jamwal P, Lal B (2006) Traditional use of medicinal plants among the tribal communities of Chhota Bhangal, Western Himalaya. J Ethnobiol Ethnomed 2:14

    Article  PubMed Central  PubMed  Google Scholar 

  22. Puri A, Srivastava AK, Singhal B, Mishra SK, Srivastava S, Lakshmi V (2011) Antidyslipidemic and antioxidant activity of Pinus roxburghii needles. Med Chem Res 20(9):1589–1593

    Article  CAS  Google Scholar 

  23. Kaushik D, Kumarm A, Kaushik P, Rana AC (2012) Analgesic and anti-inflammatory activity of Pinus roxburghii Sarg. Adv Pharmacol Sci. doi:10.1155/2012/245431

  24. Nemova EP, Smolnikova NM, Tanirbergenov TB, Durnev AD (2007) Studies on reproductive toxicity of Rumalaya. Pharm Chem J 41:399–402

    Article  CAS  Google Scholar 

  25. Khan I, Singh V, Chaudhary AK (2012) Hepatoprotective activity of Pinus roxburghii Sarg. wood oil against carbon tetrachloride and ethanol induced hepatotoxicity. Bangladesh J Pharmacol 7:94–99

    Google Scholar 

  26. Chaudhary AK, Ahmad S, Mazumder A (2012) Study of antibacterial and antifungal activity of traditional Cedrus deodara and Pinus roxburghii Sarg. TANG Int J Genuine Tradit Med 2(4):1–4

    CAS  Google Scholar 

  27. Maimoona A, Naeem I, Saddiqe Z, Ali N (2011) Analysis of total flavonoids and phenolics in different fractions of bark and needle extracts of Pinus roxburghii and Pinus wallichiana. J Med Plant Res 5(21):5216–5220

    CAS  Google Scholar 

  28. Zafar I, Fatima A, Khan SJ, Rehman Z, Mehmud S (2010) GC-MS studies of needles essential oil of Pinus roxburghii and their antimicrobial activity from Pakistan. Elec J Environ Agric Food Chem 9(3):468–473

    CAS  Google Scholar 

  29. Handa SS, Khanuja SP, Longo G, Rakesh DD (2008) Extraction technologies for medicinal and aromatic plants. International Centre for Science and High Technology, Trieste, pp 42–44

    Google Scholar 

  30. Khandelwal KR (2004) Practical pharmacognosy, Eleventh edn. Nirali Prakashan, Pune, pp 149–156

    Google Scholar 

  31. Wagner H, Bladt S, Zgainski EM (1984) Plant drug analysis––A thin layer chromatography atlas. Springer/Berlin/Heidelberg/New York pp 192–218

  32. Morris R (1984) Developments of water-maze procedure for studying spatial learning in rats. J Neurosci Meth 11:47–60

    Article  CAS  Google Scholar 

  33. Davoodi FG, Motamedi F, Naghdi N, Akbari E (2009) Effects of reversible inactivation of the reuniens nucleus on spatial learning and memory in rats using Morris water maze task. Behav Brain Res 2:130–135

    Article  Google Scholar 

  34. Rubio J, Dang H, Gong M, Liu X, Chen SL, Gonzales GF (2007) Aqueous and hydro alcoholic extracts of Black Maca (Lepidium meyenii) improve scopolamine-induced memory impairment in mice. Food Chem Toxicol 45:1882–1890

    Article  CAS  PubMed  Google Scholar 

  35. Jainkang L, Rei E, Hideaki K, Akitane M (1990) Antioxidant action of guilingi in the brain of rats with FeCl3 induced epilepsy. Free Radical Biol Med 9:451–454

    Article  Google Scholar 

  36. Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70–77

    Article  CAS  PubMed  Google Scholar 

  37. Glowinski J, Iversen LL (1966) Regional studies of catecholamine in rat brain. J Neurochem 13:655–659

    Article  CAS  PubMed  Google Scholar 

  38. Lowry H, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurements with the Folin phenol reagent. J Biol Chem 193:265–275

    CAS  PubMed  Google Scholar 

  39. Parle M, Dhingra D, Kulkarni SK (2004) Neurochemical basis of learning and memory. Indian J Pharm Sci 66:371–376

    CAS  Google Scholar 

  40. Parle M, Dhingra D, Kulkarni SK (2004) Neuromodulator of learning and memory. Asia Pac J Pharmacol 16:89–99

    CAS  Google Scholar 

  41. Rudi DH, Peter P, Deyn D (2001) Applications of the Morris water maze in the study of learning and memory. Brain Res Rev 36:60–90

    Article  Google Scholar 

  42. Coyle JT, Puttfarcven P (1993) Oxidative stress, glutamate and neurodegenerative disorder. Science 262:89–695

    Article  Google Scholar 

  43. Bast A, Haenen GR, Doelman GJ (1991) Oxidants and antioxidants: state of art. Am J Med 91:23–28

    Article  Google Scholar 

  44. Christen Y (2000) Oxidative stress and Alzheimer’s disease. Am J Clin Med 71(2):621S–629S

    CAS  Google Scholar 

  45. Sastre J, Pastardo FV, Vina J (2000) Mitochondria, oxidative stress and aging. Free Radical Res 32(3):189–198

    Article  CAS  Google Scholar 

  46. Bhatacharya SK, Satyan KS, Ghosal S (1996) Antioxidant activity of glycowithanolides from Withania Somnifera. Indian J Exp Biol 35:236–239

    Google Scholar 

  47. Schulz JB, Linderau J, Dichgans J (2000) Glutathione, oxidative stress and neurodegeneration. Eur J Biochem 276(16):4904–4911

    Article  Google Scholar 

Download references

Acknowledgments

The authors would like to thank Prof. (Dr.) Umesh Kumar Singh and other faculty members of Kharvel Subharti College of Pharmacy, S.V. Subharti University for their technical assistance.

Conflict of interest

The authors have no conflict of financial or personal interests with other people or organizations.

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Authors and Affiliations

  1. Uttarakhand Technical University, Dehradun, Uttarakhand, 248007, India

    Amrendra Kumar Chaudhary

  2. Kharvel Subharti College of Pharmacy, S.V. Subharti University, Meerut, 250005, UP, India

    Amrendra Kumar Chaudhary

  3. Translam Institute of Pharmaceutical Education and Research, Meerut, 250001, India

    Shamim Ahmad

  4. Noida Institute of Engineering & Technology, Greater Noida, 201306, India

    Avijit Mazumder

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  1. Amrendra Kumar Chaudhary
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  2. Shamim Ahmad
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  3. Avijit Mazumder
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Correspondence to Amrendra Kumar Chaudhary.

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Chaudhary, A.K., Ahmad, S. & Mazumder, A. Cognitive enhancement in aged mice after chronic administration of Cedrus deodara Loud. and Pinus roxburghii Sarg. with demonstrated antioxidant properties. J Nat Med 68, 274–283 (2014). https://doi.org/10.1007/s11418-013-0775-y

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  • DOI: https://doi.org/10.1007/s11418-013-0775-y

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