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Human Umbilical Cord-Derived Schwann-Like Cell Transplantation Combined with Neurotrophin-3 Administration in Dyskinesia of Rats with Spinal Cord Injury

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Abstract

Mesenchymal stem cells are capable of differentiating into Schwann-like cells. In this study, we induced human umbilical-cord mesenchymal stem cells (HUMSCs) in vitro into neurospheres constituted by neural stem-like cells, and further into cells bearing strong morphological, phenotypic and functional resemblances with Schwann-like cells. These HUMSC-derived Schwann-like cells, after grafting into the injured area of the rats’ spinal cord injury (SCI), showed a partial therapeutic effect in terms of improving the motor function. Neurotrophin-3 (NT-3) was reported to improve the local microenvironment of the grafted cells, and we, therefore, further tested the effect of Schwann-like cell grafting combined with NT-3 administration at the site of cell transplantation. The results showed that NT-3 administration significantly promoted the survival of the grafted cells in the host-injured area. Significant improvement in rats treated by Schwann-like cell grafting combined with NT-3 administration was demonstrated in the behavioral test as compared with that in animal models received the cell grafting only. These results suggest that transplantation of the Schwann-like cells combined with NT-3 administration may represent a new strategy of stem cell therapy for spinal cord injury.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 30901546/H0912) and Natural Science Foundation of Guangdong (No. 9451051501002508).

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Authors and Affiliations

  1. Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, 253 Gongye Road, 510282, Guangzhou, China

    Guo Yan-Wu, Ke Yi-Quan, Li Ming, Cai Ying-Qian, Jiang Xiao-Dan, Zhang Shi-Zhong, Zhang Wang-Ming & Duan Chuan-Zhi

  2. Institute of Neurosurgery, Key Laboratory on Brain Function Repair and Regeneration of Guangdong, Southern Medical University, 510282, Guangzhou, China

    Guo Yan-Wu, Ke Yi-Quan, Li Ming, Cai Ying-Qian, Jiang Xiao-Dan, Zhang Shi-Zhong, Zhang Wang-Ming & Duan Chuan-Zhi

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  1. Guo Yan-Wu
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  2. Ke Yi-Quan
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  3. Li Ming
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  4. Cai Ying-Qian
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  5. Jiang Xiao-Dan
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Correspondence to Ke Yi-Quan.

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Yan-Wu, G., Yi-Quan, K., Ming, L. et al. Human Umbilical Cord-Derived Schwann-Like Cell Transplantation Combined with Neurotrophin-3 Administration in Dyskinesia of Rats with Spinal Cord Injury. Neurochem Res 36, 783–792 (2011). https://doi.org/10.1007/s11064-011-0402-9

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  • DOI: https://doi.org/10.1007/s11064-011-0402-9

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