Abstract
R-flurbiprofen, a nonsteroidal anti-inflammatory drug derivative, has been shown to inhibit colonic adenoma formation in mice. We investigated the effects of R-flurbiprofen on cell proliferation and apoptosis in pituitary adenoma cell lines. GH4C1 rat pituitary cell line cultures and low-passage human primary pituitary cell cultures were treated with varying concentrations of R-flurbiprofen (0.1–1.0 mM). R-flurbiprofen inhibited cell proliferation in a dose-dependent fashion. A terminal deoxynucleotidyl transferase dUTP nick end labeling assay and chromatin condensation/dead cell apoptosis assay demonstrated induction of apoptosis at higher concentrations of R-flurbiprofen. R-flurbiprofen decreases cell proliferation and induces apoptosis in pituitary adenoma cells in vitro. This may be a potential therapy in the management of pituitary adenoma.
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We thank Kristin Kraus, M.Sc., for editorial assistance in preparing this article and Bradley Weaver for assistance with image analysis.
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The authors do not report any conflict of interest concerning the materials or methods used in this study or the findings specified in this article.
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Liu, J.K., Patel, S.K., Gillespie, D.L. et al. R-flurbiprofen, a novel nonsteroidal anti-inflammatory drug, decreases cell proliferation and induces apoptosis in pituitary adenoma cells in vitro. J Neurooncol 106, 561–569 (2012). https://doi.org/10.1007/s11060-011-0712-4
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DOI: https://doi.org/10.1007/s11060-011-0712-4