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Molecular cloning and expression analysis of a F-type lectin gene from Japanese sea perch (Lateolabrax japonicus)

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Abstract

The techniques of homology cloning and anchored PCR were used to clone the fucose-binding lectin (F-type lectin) gene from Japanese sea perch (Lateolabrax Japonicus). The full-length cDNA of sea perch F-lectin (JspFL) contained a 5′ untranslated region (UTR) of 39 bp, an ORF of 933 bp encoding a polypeptide of 310 amino acids with an estimated molecular mass of 10.82 kDa and a 3′ UTR of 332 bp. The searches for nucleotides and protein sequence similarities with BLAST analysis indicated that the deduced amino acid sequence of JspFL was homological to the Fucose-binding lectin in other fish species. In the JspFL deduced amino acid sequence, two tandem domains that exhibit the eel carbohydrate-recognition sequence motif were found. The temporal expressions of gene in the different tissues were measured by real-time PCR. And the mRNA expressions of the gene were constitutively expressed in tissues including spleen, head-kidney, liver, gill, and heart. The JspFL expression in spleen was different during the stimulated time point, 2 h later the expression level became up-regulated, and 6 h later the expression level became down-regulated. The result indicated that JspFL was constitutive and inducible expressed and could play a critical role in the host-pathogen interaction.

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References

  1. Ewart KV, Johnson SC, Ross NW (2001) Lectins of the innate immune system and their relevance to fish health. J Mar Sci 58:380–385

    CAS  Google Scholar 

  2. Barondes SH (1988) Bifunctional properties of lectins: lectins redefined. Trends Biochem Sci 13:480–482

    Article  PubMed  CAS  Google Scholar 

  3. Andersen O, Laursen SB, Svehag SE, Holmoskow U, Thiel S (1991) Mammalian lectins in defence mechanism against microorganism. Lectins Rev. 1:41–51

    Google Scholar 

  4. Arason GJ (1996) Lectins as defence molecules in vertebrates and invertebrates. Fish Shellfish Immunol 6:277–289

    Article  Google Scholar 

  5. Josh SS, Gabius HJ (1993) Measurement of intracellular calcium levels by flow cytometry following treatment of murine macrophage/mono-cytes with mistletoe lectin. In: Gabius HJ, Gabius S (eds) Lectins and glycobiology. Springer, Berlin

    Google Scholar 

  6. Sharon N, Lis H (1989) Lectins as cell recognition molecules. Science 246:227–233

    Article  PubMed  CAS  Google Scholar 

  7. Sharon N (1993) Lectin-carbohydrate complexes of plants and animals: an atomic view. Trends Biochem Sci 18:221–226

    Article  PubMed  CAS  Google Scholar 

  8. Drickamer K, Taylor ME (1993) Biology of animal lectins. Annu Rev Cell Biol 9:237–264

    Article  PubMed  CAS  Google Scholar 

  9. Honda S, Kashiwagi M, Miyamoto K, Takei Y, Hirose S (2000) Multiplicity, structure and endocrine and exocrine natures of eel fucose-binding lectins. J Biol Chem 275:33151–33157

    Article  PubMed  CAS  Google Scholar 

  10. Bianchet MA, Odom EW, Vasta GR, Amzel LM (2002) A novel fucose recognition fold involved in innate immunity. Nat Struct Biol 9:628–634

    PubMed  CAS  Google Scholar 

  11. Saito T, Hatada M, Iwanaga S, Kawabata S (1997) A newly identified horseshoe crab lectin with binding specificity to O-antigen of bacterial lipopolysaccharides. J Biol Chem 272:30703–30708

    Article  PubMed  CAS  Google Scholar 

  12. Macedo-Ribeiro S, Bode W, Huber R, Quinn-Allen MA, Kim SW, Ortel TL, Bourenkov GP, Bartunik HD, Stubbs MT, Kane WH, Fuentes-Prior P (1999) Crystal structures of the membrane-binding C2 domain of human coagulation factor V. Nature 402:434–439

    Article  PubMed  CAS  Google Scholar 

  13. Pratt KP, Shen BW, Takeshima K, Davie EW, Fujikawa K, Stoddard BL (1999) Structure of the C2 domain of human factor VIII at 1.5 A resolution. Nature 402:439–442

    Article  PubMed  CAS  Google Scholar 

  14. Gaskell A, Crennell S, Taylor G (1995) The three domains of a bacterial sialidase: a beta-propeller, an immunoglobulin module and a galactose-binding jelly-roll. Structure (Camb.) 3:1197–1205

    Article  CAS  Google Scholar 

  15. Ito N, Phillips SE, Stevens C, Ogel ZB, McPherson MJ, Keen JN, Yadav KD, Knowles PF (1991) Novel thioether bond revealed by a 1.7 A crystal structure of galactose oxidase. Nature 350:87–90

    Article  PubMed  CAS  Google Scholar 

  16. Lee CC, Kreusch A, McMullan D, Ng K, Spraggon G (2003) Crystal structure of the human neuropilin-1 b1 domain. Structure (Camb.) 11:99–108

    Article  CAS  Google Scholar 

  17. Leulliot N, Quevillon-Cheruel S, Sorrel I, Graille M, Meyer P, Liger D, Blondeau K, Janin J, Tilbeurgh H (2004) Crystal structure of yeast allantoicase reveals a repeated jelly roll motif. J Biol Chem 279:23447–23452

    Article  PubMed  CAS  Google Scholar 

  18. Shao ZT, Cong X, Yuan JD, Yang GW, Chen Y, Pan J, An LG (2009) Construction and characterization of a cDNA library from head kidney of Japanese sea bass (Lateolabrax japonicus). Mol Biol Rep 36(7):2031–2037

    Article  PubMed  CAS  Google Scholar 

  19. Qiu L, Zhang H, Yang K, Jiang S (2009) Molecular cloning and mRNA expression analysis of interleukin-8 gene in Japanese sea perch (Lateolabrax japonicus). Mol Biol Rep 36(5):1099–1105

    Article  PubMed  CAS  Google Scholar 

  20. Cheng HL, Sun SP, Peng YX, Shi XY, Shen X, Meng XP, Dong ZG (2009) cDNA sequence and tissues expression analysis of lipoprotein lipase from common carp (Cyprinus carpio Var. Jian). Mol Biol Rep 37(6):2665–2673

    Article  PubMed  Google Scholar 

  21. Inagawa H, Kuroda A, Nishizawa T et al (2001) Cloning and characterization of tandem-repeat type galectin in rainbow trout (Oncorhynchus mykiss). Fish Shellfish Immunol 11:217–231

    Article  PubMed  CAS  Google Scholar 

  22. Cammareta M, Vazzana M, Chinnici C et al (2001) A serum fucolectin isolated and characterized from sea bass Dicentrarchus labrax. Biochim Biophys Acta 1528:196–202

    Google Scholar 

  23. Odom EW, Vasta GR (2006) Characterization of a binary tandem domain f-type lectin from striped bass (Morone saxatilis). J Biol Chem 281(3):1698–1713

    Article  PubMed  CAS  Google Scholar 

  24. Thiel S, Vourp Jensen T, Stover CM, Schwable W, Eggleton P, Hansen S, Holmoskov U, Reid KB, Jensunius JC (1997) A second serine protease associated with mannose-binding lectin that activates complement. Nature 386:506–510

    Article  PubMed  CAS  Google Scholar 

  25. Ikeda K, Sannoh T, Kawasaki T, Kawasaki N, Yamashina I (1987) Serum lectin with known structure activates complement through the classical pathway. J Biol Chem 262:7451–7454

    PubMed  CAS  Google Scholar 

  26. Faisal M, Chiappelli F, Ahmed II, Cooper EL, Weiner H (1989) Social confrontation ‘stress’ in aggressive fish is associated with an endogenous opioid-mediated suppression of proliferative response to mitogens and non-specific cytotoxicity. Brain Behav Immun 3:223–233

    Article  PubMed  CAS  Google Scholar 

  27. Odom E, Vasta GR (2006) Characterization of a binary tandem domain F-type lectin from striped bass (Morone saxatilis). J Biol Chem 281:1698–1713

    Article  PubMed  CAS  Google Scholar 

  28. Chothia C, Lesk AM (1986) The relation between the divergence of sequence and structure in proteins. EMBO J 5:823–826

    PubMed  CAS  Google Scholar 

  29. Voss EW Jr, Fryer JL, Banowetz GM (1978) Isolation, purification, and partial characterization of a lectin of a Chinook salmon ova. Arch Biochem Biophys 186:25–34

    Article  PubMed  CAS  Google Scholar 

  30. Krajhanzl A (1990) Egg lectin of invertebrates and lower vertebrates: properties and biological function. Adv Lectin Res 3:83–131

    CAS  Google Scholar 

  31. Salerno G, Parisi MG, Parrinello D, Benenati G, Vizzini A, Vazzana M, Vasta GR, Cammarata M (2009) F-type lectin from the sea bass (Dicentrarchus labrax): purification, cDNA cloning, tissue expression and localization, and opsonic activity. Fish Shellfish Immunol 27:143–153

    Article  PubMed  CAS  Google Scholar 

  32. Iwama G, Nakanishi T (1996) The fish immune system—organism, pathogen, environment. Academic Press, New York

    Google Scholar 

  33. Ulevitch RJ, Tobias PS (1995) Receptor-dependent mechanisms of cell stimulation by bacterial endotoxin. Annu Rev Immunol 13:437–457

    Article  PubMed  CAS  Google Scholar 

  34. Lemaitre B, Nicolas E, Michaut L, Reichhart JM, Hoffmann JA (1996) The dorsoventral regulatory gene cassette spatzle/Toll/cactus controls the potent antifungal response in Drosophila adults. Cell 86:973–983

    Article  PubMed  CAS  Google Scholar 

  35. Ostos MA, Recalde D, Zakin MM, Scott-Algara D (2002) Implication of natural killer T cells in atherosclerosis development during a LPS induced chronic inflammation. FEBS Lett 519:23–29

    Article  PubMed  CAS  Google Scholar 

  36. Kuhlman M, Joiner K, Ezekowitz AB (1989) The human mannose-binding protein functions as an opsonin. J Exp Med 169:1733–1745

    Article  PubMed  CAS  Google Scholar 

  37. Cooper D, Butcher CM, Berndt MC, Vadas MA (1994) P-selectin interacts with a beta 2-integrin to enhance phagocytosis. J Immunol 153:3199–3209

    PubMed  CAS  Google Scholar 

  38. Tino MJ, Wright JR (1996) Surfactant protein A stimulates phagocytosis of specific pulmonary pathogens by alveolar macrophages. Am J Physiol 270:677

    Google Scholar 

  39. Goldstein IJ, Huges RC, Monsigny M, Osawa T, Sharon N (1980) What should be called a lectin? Nature 285:66

    Article  Google Scholar 

  40. Rabinovich GA, Toscano MA, Jackson SS, Vasta GR (2007) Functions of cell surface galectin–glycoprotein lattices. Curr Opin Struct Biol 17:513–520

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This work was supported by a grant from the GuangDong Province of China (2005B20301023).

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Authors and Affiliations

  1. Biotechnology and Aquiculture Laboratory, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 231 Xingangxi Road, Guangzhou, 510300, People’s Republic of China

    Lihua Qiu, Keng Yang, Hanhua Zhang, Jianzhu Li, Falin Zou & Shigui Jiang

  2. Chinese Academy of Fishery Sciences, Beijing, 100039, People’s Republic of China

    Liansheng Lin

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  1. Lihua Qiu
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  2. Liansheng Lin
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  3. Keng Yang
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  4. Hanhua Zhang
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  5. Jianzhu Li
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  6. Falin Zou
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  7. Shigui Jiang
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Correspondence to Shigui Jiang.

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Lihua Qiu and Liansheng Lin are contributed equally to this work.

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Qiu, L., Lin, L., Yang, K. et al. Molecular cloning and expression analysis of a F-type lectin gene from Japanese sea perch (Lateolabrax japonicus). Mol Biol Rep 38, 3751–3756 (2011). https://doi.org/10.1007/s11033-010-0490-7

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  • DOI: https://doi.org/10.1007/s11033-010-0490-7

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