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SOn-column Protein Refolding for Crystallization

  • Published:
Journal of Structural and Functional Genomics

Abstract

One major bottleneck in protein production in Escherichia coli for structural genomics projects is the formation of insoluble protein aggregates (inclusion bodies). The efficient refolding of proteins from inclusion bodies is becoming an important tool that can provide soluble native proteins for structural and functional studies. Here we report an on-column refolding method established at the Berkeley Structural Genomics Center (BSGC). Our method is a combination of an ‘artificial chaperone-assisted refolding’ method previously proposed and affinity chromatography to take advantage of a chromatographic step: less time-consuming, no filtration or concentration, with the additional benefit of protein purification. It can be easily automated and formatted for high-throughput process.

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Authors and Affiliations

  1. Berkeley Structural Genomics Center, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, 94720, USA

    Natalia Oganesyan, Sung-Hou Kim & Rosalind Kim

  2. Department of Chemistry, University of California, Berkeley, California, 94720, USA

    Sung-Hou Kim

Authors
  1. Natalia Oganesyan
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  2. Sung-Hou Kim
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  3. Rosalind Kim
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Correspondence to Rosalind Kim.

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Oganesyan, N., Kim, SH. & Kim, R. SOn-column Protein Refolding for Crystallization. J Struct Funct Genomics 6, 177–182 (2005). https://doi.org/10.1007/s10969-005-2827-3

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  • DOI: https://doi.org/10.1007/s10969-005-2827-3

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