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Effects of Free Ca2+ on Kinetic Characteristics of Holotransketolase

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Abstract

Catalytic activity has been demonstrated for holotransketolase in the absence of free bivalent cations in the medium. The two active centers of the enzyme are equivalent in both the catalytic activity and the affinity for the substrates. In the presence of free Ca2+ (added to the medium from an external source), this equivalence is lost: negative cooperativity is induced on binding of either xylulose 5-phosphate (donor substrate) or ribose 5-phosphate (acceptor substrate), whereupon the catalytic conversion of the bound substrates causes the interaction between the centers to become positively cooperative. Moreover, the enzyme total activity increase is observed.

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Fig. 1
Fig. 2

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Notes

  1. Here, as in Fig. 2, we show data of one reproducible experiment.

Abbreviations

TK:

Transketolase

TDP:

Thiamine diphosphate

R5P:

Ribose 5-phosphate

X5P:

Xylulose 5-phosphate

References

  1. Esakova OA, Meshalkina LE, Kochetov GA (2005) Life Sci 78:8–13

    Article  CAS  Google Scholar 

  2. Heinrich CP, Noack K, Wiss O (1972) Biochem Biophys Res Commun 49:1427–1432

    Article  CAS  Google Scholar 

  3. Heinrich C, Schmidt D (1973) Experientia 29:1226–1227

    Article  CAS  Google Scholar 

  4. Heinrich H, Steffen H, Janser P, Wiss O (1972) Eur J Biochem 30:533–541

    Article  CAS  Google Scholar 

  5. Kochetov GA (1982) Methods Enzym 90:209–223

    Article  CAS  Google Scholar 

  6. Kochetov GA, Philippov PP (1970) Biochem Biophys Res Commun 38:930–933

    Article  CAS  Google Scholar 

  7. Kochetov GA, Philippov PP (1970) Dokl Acad Nauk USSR 19:1234–1236

    Google Scholar 

  8. Kochetov GA, Meshalkina LE, Usmanov RA (1976) Biochem Biophys Res Commun 69:836–843

    Article  Google Scholar 

  9. Kochetov GA, Tikhomirova NK, Philippov PP (1975) Biochem Biophys Res Commun 63:924–930

    Article  CAS  Google Scholar 

  10. Lindqvist Y, Schneider G, Ermler V, Sundström M (1992) EMBO J 11:2373–2379

    CAS  Google Scholar 

  11. Meshalkina LE, Kochetov GA (1979) Dokl Acad Nauk USSR 248:1482–1486

    CAS  Google Scholar 

  12. Nikkola M, Lindqvist Y, Schneider G (1994) J Mol Biol 238:387–404

    Article  CAS  Google Scholar 

  13. Philippov PP, Tikhomirova NK, Kochetov GA (1978) Physico-Chem Methods of Mol Biol pp 57–64, Moscow State University, Moscow

  14. Selivanov VA, Kovina MV, Kochevova NV, Meshalkina LE, Kochetov GA (2004) FEBS Lett 567:270–274

    Article  CAS  Google Scholar 

  15. Selivanov VA, Kovina MV, Kochevova NV, Meshalkina LE, Kochetov GA (2003) J Mol Catal. B Enzym 26:33–40

    Article  CAS  Google Scholar 

  16. Schneider G, Lindqvist Y (1998) Biochim Biophys Acta 1385:387–398

    Article  CAS  Google Scholar 

  17. Schenk G, Duggleby RG, Nixon PF (1998) Int J Biochem Cell Biol 30:1297–1318

    Article  CAS  Google Scholar 

  18. Solovieva ON (2002) Biochemistry (Moscow) 67:667–671

    Article  Google Scholar 

  19. Solovieva ON, Bykova IA, Meshalkina LE, Kovina MV, Kochetov GA (2001) Biochemistry (Moscow) 66:932–936

    Article  Google Scholar 

  20. Solovieva ON, Kochetov GA (2008) J Mol Catal B Enzym 54:90–92

    Article  Google Scholar 

  21. Sundström M, Lindqvist Y, Schneider G, Hellman U, Ronne H (1993) J Biol Chem 268:24346–24352

    Google Scholar 

  22. Yonatt J (1993) Critical Rev Microbiol 19:83–97

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by a grant from the Russian Foundation for Basic Research (projects No. 09-04-00544).

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Authors and Affiliations

  1. A.N. Belozersky Institute of Physico-chemical Biology, Moscow State University, Moscow, 119992, Russia

    Olga N. Solovjeva, Irina A. Sevostyanova, Vladimir A. Yurshev, Vitalii A. Selivanov & German A. Kochetov

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  1. Olga N. Solovjeva
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  2. Irina A. Sevostyanova
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  3. Vladimir A. Yurshev
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  4. Vitalii A. Selivanov
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  5. German A. Kochetov
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Correspondence to German A. Kochetov.

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Solovjeva, O.N., Sevostyanova, I.A., Yurshev, V.A. et al. Effects of Free Ca2+ on Kinetic Characteristics of Holotransketolase. Protein J 31, 137–140 (2012). https://doi.org/10.1007/s10930-011-9382-5

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  • DOI: https://doi.org/10.1007/s10930-011-9382-5

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