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CD4 +CD25 + Regulatory T Cells Decreased the Antitumor Activity of Cytokine-Induced Killer (CIK) Cells of Lung Cancer Patients

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CD4 +CD25 + regulatory T cells (Tregs) have been shown to inhibit cytotoxic lymphocytes-mediated immune responses. Cytokine-induced killer (CIK) cells exert high impact on adoptive immunotherapeutic approaches. Therefore, the purpose of this report was to determine the effect of Tregs on CIK cell growth and CIK-induced cytotoxicity for inhibition of tumor growth in vivo as well as in vitro. After depletion of CD4 +CD25 + cells before culture, the proliferation and cytotoxicity of CIK cells, which indicated in bromodeoxyuridine (BrdU) and lactic dehydrogenase (LDH) assays, were significantly increased. Depletion of CD4 +CD25 + cells preculture also enhanced the suppression effect on the lung cancer cells inoculated in experimental animals. Blockage of glucocorticoid-induced tumor necrosis factor receptor (GITR) and transforming growth factor β1 (TGF-β1) by antibodies partially abrogated the suppressive effect of CD4 +CD25 + cells on CIK. These results indicated that Tregs could inhibit the antitumor activity of CIK cells. The molecules TGF-β and GITR may contribute to the suppressive function of CD4 +CD25 + cells.

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ACKNOWLEDGMENTS

We thank Dr. Yan Fang, Department of Pediatrics Division of Gastroenterology, Hepatology and Nutrition, Vanderbilt University School of Medicine for providing help and assistance for this paper.

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  1. Department of Immunology, Cancer Institute and Hospital, Tianjin Medical University, Tianjin, 30060, China

    Hui Li, Jin-Pu Yu, Shui Cao, Feng Wei, Peng Zhang, Xiu-Mei An, Zong-Tang Huang & Xiu-Bao Ren

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  1. Hui Li
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  2. Jin-Pu Yu
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Correspondence to Xiu-Bao Ren.

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Li, H., Yu, JP., Cao, S. et al. CD4 +CD25 + Regulatory T Cells Decreased the Antitumor Activity of Cytokine-Induced Killer (CIK) Cells of Lung Cancer Patients. J Clin Immunol 27, 317–326 (2007). https://doi.org/10.1007/s10875-007-9076-0

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  • DOI: https://doi.org/10.1007/s10875-007-9076-0

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