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Thermosensitive behavior in cell culture media and cytocompatibility of a novel copolymer: poly(N-isopropylacrylamide-co-butylacrylate)

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Abstract

Cell sheet technology is a promising step forward in tissue engineering. Cell sheets are usually generated using Poly(N-isopropylacrylamide) hydrogels due to their swelling change around the lower critical solution temperature (LCST). Nevertheless, LCST can be affected by cell culture medium components and therefore it is necessary to ensure that the polymer preserves its thermosensitivity under these conditions. We propose a novel thermosensitive crosslinked-copolymer: Poly(N-isopropylacrylamide-co-butylacrylate). This copolymer is shown to be cytocompatible and thermosensitive under cell culture medium conditions, and besides, it can be synthesized inexpensively. Thermosensitivity was investigated by determining the LCST with differential scanning calorimetry and swelling/ratio measurements. Cytocompatibility and capacity to deliver cell sheets were studied employing 3T3 and human oral epithelial cells. In conclusion, we obtained a thermosensitive copolymer that allows cell sheet formation/detachment by using a simple and low-cost polymerization method. Furthermore, crosslinking allows easy manipulation of cell sheets growing on the copolymer for potential in situ applications.

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Acknowledgments

Natalia Becerra was supported by Colciencias, for the fellowship in the National Doctoral 2008 Program. This work has been supported by an internal project of the Medicine (2008) and CODI-Sostenibilidad project (2012–2013), University of Antioquia. Special thanks to Dr. Luis Fernando Correa for the histological analyses, Dr. Manuel Espinosa from the Electronic Microcopy Laboratory for the SEM analyses and Dr. Lorenzo Moroni from University of Twente for valuable comments to the manuscript and their helpful suggestions for changes.

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Authors and Affiliations

  1. Grupo Ciencia de los Materiales, Sede de Investigación Universitaria (SIU), Universidad de Antioquia, Cll 62 # 52-59 Torre 1 Laboratorio 310-311, Medellín, Colombia

    Natalia Y. Becerra & Betty L. López

  2. Grupo Ingeniería de Tejidos y Terapias celulares, Facultad de Medicina Carerra, Universidad de Antioquia, 51A No-62-42, Medellín, Colombia

    Natalia Y. Becerra & Luz M. Restrepo

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  1. Natalia Y. Becerra
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  2. Betty L. López
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  3. Luz M. Restrepo
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Correspondence to Natalia Y. Becerra.

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Becerra, N.Y., López, B.L. & Restrepo, L.M. Thermosensitive behavior in cell culture media and cytocompatibility of a novel copolymer: poly(N-isopropylacrylamide-co-butylacrylate). J Mater Sci: Mater Med 24, 1043–1052 (2013). https://doi.org/10.1007/s10856-013-4861-1

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  • DOI: https://doi.org/10.1007/s10856-013-4861-1

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