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Creation of macroporous calcium phosphate cements as bone substitutes by using genipin-crosslinked gelatin microspheres

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Abstract

Macroporous calcium phosphate cements (CPCs) were developed using genipin-crosslinked gelatin microspheres (GMs) with two weight ratios (2.5 wt% and 5 wt%). The initial setting time of the composite was prolonged by GMs. After GMs/CPCs were soaked in phosphate-buffered saline (PBS) for several weeks, macropores appeared as a result of the degradation of GMs. The presence of GMs accelerated the setting reaction and improved the structure of the composite. The compressive strength increased up to 12 MPa (2.5 wt% GMs/CPCs) and 14 MPa (5 wt% GMs/CPCs) after one week of PBS soaking, then gradually decreased to 9 MPa (2.5 wt% GMs/CPCs) and 7 MPa (5 wt% GMs/CPCs) after three weeks of soaking, and further to 6 MPa (2.5 wt% GMs/CPCs) and 2 MPa (5 wt% GMs/CPCs) after five weeks of soaking. CPCs with 2.5 wt% GMs were the most favorable composite in the tested samples. Cell experiments showed that rat osteoblasts displayed normal morphologies when exposed to the 2.5 wt% GMs/CPCs, and proliferation of the cells was also enhanced. An in vivo study showed that new bone tissue was able to grow into the pores that resulted from GM degradation. This study suggests that the new composite could be a promising candidate for use as a bone substitute under non-compression-loaded circumstances.

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Acknowledgments

This work was funded by Medical Science Research Foundation of Chinese People’s Liberation Army (Grant Number of 06MA090) and Natural Science Foundation of Gansu Province (Grant Number of 0710RJZA068). CPCs were kindly supplied by Shanghai Rebone Biomaterials (China).

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Authors and Affiliations

  1. Orthopedic Institute of Chinese People’s Liberation Army, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032, People’s Republic of China

    Meng Li

  2. Orthopedic Trauma Institute of Chinese People’s Liberation Army, Lanzhou General Hospital, Lanzhou, Gansu Province, 730050, People’s Republic of China

    Xingyan Liu, Xudong Liu, Baofeng Ge & Keming Chen

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  1. Meng Li
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  2. Xingyan Liu
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Correspondence to Xingyan Liu.

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Li, M., Liu, X., Liu, X. et al. Creation of macroporous calcium phosphate cements as bone substitutes by using genipin-crosslinked gelatin microspheres. J Mater Sci: Mater Med 20, 925–934 (2009). https://doi.org/10.1007/s10856-008-3654-4

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