Abstract
A study of the fatty acid composition was made for 35 Arthrospira strains, concentrating on the most abundant fatty acids, the two polyunsaturated C18 acids, linoleic and γ-linolenic acid, and palmitic acid. When grown at 30 ∘C and low irradiance (10 μmol photon m−2 s−1), these three acids together formed 88–92% of total fatty acids. There were considerable differences in the composition of the two polyunsaturated acids. Depending on the strain, linoleic acid formed 13.1–31.5% and γ-linolenic acid formed 12.9–29.4% total fatty acids. In contrast, the range for palmitic acid was narrow: 42.3–47.6% of total fatty acids. Repeat experiments on several strains under defined conditions led to closely similar results for any particular environment, suggesting that fatty acid composition can be used as an aid in differentiating between strains. Five additional strains, which had apparently originated from the same original stock cultures as 3 of the 35 in the main study, but from different culture collections, were also assayed. With four strains the results were similar, irrespective of culture source, but with one strain marked differences occurred, especially in the polyunsaturated C18 fatty acid fraction. These differences were independent of the age of the culture. In addition, straight morphotypes derived during repeat subcultures of four strains; each showed a similar fatty acid composition to that of the helical morphotypes of the same strains. A decrease in temperature from 30 to 20 ∘C, an increase in irradiance (at 30 ∘C) from 10 to 70 μmol photon m−2 s−1 and transfer to dark heterotrophy all favoured an increase in polyunsaturated C18 fatty acids. The highest γ-linolenic acid content of any conditions was found for three strains grown heterotrophically on glucose in the dark at 30 ∘C. A comparative study of six strains of Spirulina confirmed a previous study showing the absence of γ-linolenic acid in all Spirulina strains, thus permitting the separation of these two genera.
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References
Belay A (2002) The potential application of Spirulina (Arthrospira) as a nutritional and therapeutic supplement in health management. J. Am. Nutraceutical Assoc. 5: 27–48.
Cohen Z, Reungjitchachawali M, Siangdung W, Tanticharoen M (1993) Production and partial purification of γ-Linolenic acid and some pigments from Spirulina platensis. J. Appl. Phycol. 5: 109–115.
Cohen Z, Vonshak A, Richmond A (1987) Fatty acid composition of Spirulina strains grown under various environmental conditions. Phytochemistry 26: 2255–2258.
Colla LM, Bertolini TE, Vosta JAV (2004) Fatty acids profile of Spirulina platensis grown under different temperatures and nitrogen concentrations. Z. Naturforsch. 59c: 55–59.
Coolbear KP, Berde CB, Keough KMW (1983) Gel to liquid-crystalline phase transition of aqueous dispersions of polyunsaturated mixed-acid phosphatidylcholines. Biochemistry 22: 1466–1473.
Deshnium P, Paithoonrangsarid K, Suphatrakul A, Meesapyodsuk D, Tanticharoen M, Cheevadhanarak S (2000) Temperature-independent and -dependent expression of desaturase genes in filamentous cyanobacterium Spirulina platensis strain C1 (Arthrospira sp. PCC9438). FEMS Microbiol. Letts 184: 207–213.
Hirano M, Mori H, Miura Y, Matsunaga N, Nakamura N, Matsunaga T (1990) γ-Linolenic acid production by microalgae. Appl. Biochem. Biotechnol. 24: 183–191.
Leaf A, Weber PC (1988) Cardiovascular effects of n-3 fatty acids. N. Engl. J. Med. 318: 549–557.
Mühling M, Harris N, Belay A, Whitton BA (2003) Reversal of helix orientation in the cyanobacterium Arthrospira. J. Phycol. 39: 360–367.
Murata N, Deshnium P, Tasaka Y (1996) Biosynthesis of γ-linolenic acid in the cyanobacterium Spirulina platensis. In Huang Y-S, Mills DE (eds), γ-Linolenic Acid – Metabolism and its Role in Nutrition and Medicine, AOCS Press, Champaign, Illinois, USA, pp. 22–32.
Murata N, Nishida I (1987) Lipids of blue-green algae (cyanobacteria). In Stumpf PK (ed) The Biochemistry of Plants, Vol. 9., Academic Press, San Diego, USA, pp. 315–347.
Murata N, Ono T-A, Sato N (1979) Lipid phase of membrane and chilling injury in the blue-green alga, Anacystis nidulans. In Lyons JM, Graham D, Radison JK (eds), Low Temperature Stress in Crop Plants: The Role of the Membrane, Academic Press, New York, pp. 337–345.
Olguin E, Galicia S, Angulo-Guerrero O, Hern⋅ndez E (2001) The effect of low light flux and nitrogen deficiency on the chemical composition of Spirulina sp. (Arthrospira) grown on digested pig waste. Biores. Technol. 77: 19–24.
Scheldeman P, Baurain D, Bouhy R, Scott M, Mühling M, Whitton BA, Belay A, Wilmotte A (1999) Arthrospira (“Spirulina”) strains from four continents are resolved into only two clusters, based on amplified ribosomal DNA restriction analysis of the internally transcribed spacer. FEMS Microbiol. Letts 172: 213–222.
Tanticharoen M, Reungjitchachawali M, Boonag B, Vonktaveesuk P, Vonshak A, Cohen Z (1994) Optimization of γ-linolenic acid (GLA) production in Spirulina platensis. J. appl. Phycol. 6: 295–300.
Tasaka Y, Gombos Z, Nishiyama Y, Mohanty P, Ohba T, Ohki K, Muarata N (1996) Targeted mutagenesis of acyl-lipid desaturases in Synechocystsis: Evidence for the roles of polyunsaturated membrane lipids in growth, respiration and photosynthesis. EMBO J. 15: 6414–6425.
Tedesco M, Duerr E (1989) Light, temperature and nitrogen starvation effects on the total lipid and fatty acid content and composition of Spirulina platensis UTEX 1928. J. appl. Phycol. 1: 201–209.
Tomaselli L, Palandri MR, Tredici MR (1996) On the correct use of the Spirulina designation. Arch. Hydrobiol. (Suppl.) 100/Algologic. Stud. 83: 539–548.
Van Damme P, Pot B, Gillis M, De Vos P, Kersters K, Swings J. (1996) Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol. Rev. 60: 407–438.
Wada H, Gombos Z, Murata N (1994) Contribution of membrane lipids to the ability of the photosynthetic machinery to tolerate temperature stress. Proc. natl. Acad. Sci. USA 91: 4273–4277.
Waterbury JB, Stanier RY (1981) Isolation and growth of cyanobacteria from marine and hypersaline environments. In Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG (eds), The Prokaryotes, Vol. 1, Springer-Verlag, Berlin, pp. 247–256.
Wu D, Meydani N (1996) γ-Linolenic acid and immune function. In Huang Y-S, Mills DE (eds), γ-Linolenic Acid – Metabolism and its Role in Nutrition and Medicine. AOCS Press, Champaign, Illinois, pp. 106–117.
Zarrouk C (1966) Contribution à l’étude d’une cyanophycée. Influence de divers facteurs physiques et chimiques sur la croissance et la photosynthèse de Spirulina maxima (Setch. et Gardner) Geitl. Ph.D. Thesis, Paris.
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Mühling, M., Belay, A. & Whitton, B.A. Variation in fatty acid composition of Arthrospira (Spirulina) strains. J Appl Phycol 17, 137–146 (2005). https://doi.org/10.1007/s10811-005-7213-9
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DOI: https://doi.org/10.1007/s10811-005-7213-9