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p75NTR-Immunoreactivity in the subventricular zone of adult male rats: Expression by cycling cells

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Abstract

While the study of in vitro regulation of neural stem cell lineage from both embryonic and adult neurospheres is greatly advanced, much less is known about factors acting in situ for neural stem cell lineage in adult brain. We reported that neurotrophin low affinity receptor p75NTR is present in the subventricular zone (SVZ) in adult male rats. We then characterized co-distribution of markers associated with precursor cells (nestin and PSA-NCAM) with growth factor receptors (p75NTR, trkA, EGFr) and proliferation-associated antigens (Ki67 and BrDU-uptake) in adult male rat by immunocytochemistry and confocal laser scan microscopy. Distribution of p75NTR-immunoreactivity (IR) was investigated using different mono- and polyclonal antisera. p75NTR− is not co-distributed with glial fibrillary acid protein. It was found to be co-distributed with a small number of nestin-IR cells, whereas no coexistence with PSA-NCAM-IR was observed. Conversely, p75NTR-IR was present in numerous dividing cells (Ki-67-positive) and co-distributed with EGFr. In order to verify the possible association between p75NTR and cell death, we investigated co-distribution of p75NTR-IR with nuclear condensation images as visualized by Hoechst 33258 staining. While few images indicating nuclear condensation were observed in the SVZ, no coexistence with p75NTR was found. TrkA- and trkB-IR was not found in the SVZ. We also investigated p75NTR immunostaining on post-natal day 1 and day 16, because of the dramatic reduction of proliferating cells in SVZ over this time-interval. p75NTR-IR was not increased in the early post-natal phase. Thus, p75NTR seems to be associated with cell cycle regulation in SVZ in adult rat brain.

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References

  • G Alonso (1999) ArticleTitleNeuronal progenitor-like cells expressing polysialylated neural cell adhesion molecule are present on the ventricular surface of the adult rat brain and spinal cord J Comp Neurol 414 149–166

    Google Scholar 

  • A Alvarez-Buylla JM Garcia-Verdugo (2002) ArticleTitleNeurogenesis in adult subventricular zone J Neurosci 22 629–634

    Google Scholar 

  • A Alvarez-Buylla C Lois (1995) ArticleTitleNeuronal stem cells in the brain of adult vertebrates Stem Cells 13 263–272

    Google Scholar 

  • PA Barker NK Hussain PS McPherson (2002) ArticleTitleRetrograde signaling by the neurotrophins follows a well-worn trk Trends Neurosci 25 379–381

    Google Scholar 

  • F Barnabé-Heider FD Miller (2003) ArticleTitleEndogenously produced neurotrophins regulate survival and differentiation of cortical progenitors via distinct signaling pathways J Neurosci 23 5149–5160

    Google Scholar 

  • WP Bartlett XS Li M Williams (1992) ArticleTitleExpression of IGF-1 mRNA in the murine subventricular zone during postnatal development Mol Brain Res. 12 285–291

    Google Scholar 

  • I Benzel YA Barde E Casademunt (2001) ArticleTitleStrain-specific complementation between NRIF1 and NRIF2, two zinc finger proteins sharing structural and biochemical properties Gene 281 19–30

    Google Scholar 

  • L Bonfanti P Peretto A Merighi A Fasolo (1997) ArticleTitleNewly-generated cells from the rostral migratory stream in the accessory olfactory bulb of the adult rat Neurosci 81 489–502

    Google Scholar 

  • AR Calver AC Hall WP Yu FS Walsh JK Heath C Betsholtz WD Richardson (1998) ArticleTitleOligodendrocyte population dynamics and the role of PDGF in vivo Neuron 20 869–882

    Google Scholar 

  • L Calza L Giardino M Pozza C Bettelli A Micera L Aloe (1998) ArticleTitleProliferation and phenotype regulation in the subventricular zone during experimental allergic encephalomyelitis: in vivo evidence of a role for nerve growth factor Proc Natl Acad Sci USA 95 3209–3214

    Google Scholar 

  • L Calza M Fernandez A Giuliani L Aloe L Giardino (2002) ArticleTitleThyroid hormone activates oligodendrocyte precursors and increases a myelin-forming protein and NGF content in the spinal cord during experimental allergic encephalomyelitis Proc Natl Acad Sci USA 99 3258–3263

    Google Scholar 

  • L Calza A Giuliani M Fernandez S Pirondi G D’Intino L Aloe L Giardino (2003) ArticleTitleNeural stem cells and cholinergic neurons: regulation by immunolesion and treatment with mitogens, retinoic acid, and nerve growth factor Proc Natl Acad Sci USA 100 7325–7330

    Google Scholar 

  • HA Cameron RD McKay (2001) ArticleTitleAdult neurogenesis produces a large pool of new granule cells in the dentate gyrus J Comp Neurol 435 406–417

    Google Scholar 

  • E Casademunt BD Carter I Benzel JM Frade G Dechant YA Barde (1999) ArticleTitleThe zinc finger protein NRIF interacts with the neurotrophin receptor p75(NTR) and participates in programmed cell death EMBO J 18 6050–6061

    Google Scholar 

  • E Cattaneo R McKay (1990) ArticleTitleProliferation and differentiation of neuronal stem cells regulated by nerve growth factor Nature 347 762–765

    Google Scholar 

  • A Cheng S Wang J Cai MS Rao MP Mattson (2003) ArticleTitleNitric oxide acts in a positive feedback loop with BDNF to regulate neural progenitor cell proliferation and differentiation in the mammalian brain Dev Biol 258 319–333

    Google Scholar 

  • A Chittka MV Chao (1999) ArticleTitleIdentification of a zinc finger protein whose subcellular distribution is regulated by serum and nerve growth factor Proc Natl Acad Sci USA 96 10705–10710

    Google Scholar 

  • MH Cortazzo ES Kassis KA Sproul NF Schor (1996) ArticleTitleNerve growth factor (NGF)-mediated protection of neural crest cells from antimitotic agent-induced apoptosis: the role of the low-affinity NGF receptor J Neurosci 16 3895–3899

    Google Scholar 

  • F Doetsch A Alvarez-Buylla (1996) ArticleTitleNetwork of tangential pathways for neuronal migration in adult mammalian brain Proc Natl Acad Sci USA 93 14895–14900

    Google Scholar 

  • F Doetsch JM Garcia-Verdugo A Alvarez-Buylla (1997) ArticleTitleCellular composition and three-dimensional organization of the subventricular germinal zone in the adult mammalian brain J Neurosci 17 5046–5061

    Google Scholar 

  • F Doetsch JM Garcia-Verdugo A Alvarez-Buylla (1999) ArticleTitleRegeneration of a germinal layer in the adult mammalian brain Proc Natl Acad Sci USA 96 11619–11624

    Google Scholar 

  • J Fallon S Reid R Kinyamu I Opole R Opole J Baratta M Korc TL Endo A Duong G Nguyen M Karkehabadhi D Twardzik S Patel S Loughlin (2000) ArticleTitleIn vivo induction of massive proliferation, directed migration, and differentiation of neural cells in the adult mammalian brain Proc Natl Acad Sci USA 97 14686–14691

    Google Scholar 

  • JM Frade (2000a) ArticleTitleNRAGE and the cycling side of the neurotrophin receptor p75 Trends Neurosci. 23 591–592

    Google Scholar 

  • JM Frade (2000b) ArticleTitleUnscheduled re-entry into the cell cycle induced by NGF precedes cell death in nascent retinal neurones J Cell Sci 113 1139–1148

    Google Scholar 

  • FH Gage J Ray LJ Fisher (1995) ArticleTitleIsolation, characterization, and use of stem cells from the CNS Annu Rev Neurosci 18 159–192

    Google Scholar 

  • L Giardino C Bettelli L Calza (2000) ArticleTitleIn vivo regulation of precursor cells in the subventricular zone of adult rat brain by thyroid hormone and retinoids Neurosci Lett 295 17–20

    Google Scholar 

  • E Gould CG Gross (2002) ArticleTitleNeurogenesis in adult mammals: some progress and problems J Neurosci 22 619–623

    Google Scholar 

  • LA Greene AS Tischler (1976) ArticleTitleEstablishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor Proc Natl Acad Sci USA 73 2424–2428

    Google Scholar 

  • F Hofstadter R Knuchel J Ruschoff (1995) ArticleTitleCell proliferation assessment in oncology Virchows Arch 427 323–341

    Google Scholar 

  • PJ Horner AE Power G Kempermann HG Kuhn TD Palmer J Winkler LJ Thal FH Gage (2000) ArticleTitleProliferation and differentiation of progenitor cells throughout the intact adult rat spinal cord J Neurosci 20 2218–2228

    Google Scholar 

  • S Hosomi T Yamashita M Aoki M Tohyama (2003) ArticleTitleThe p75 receptor is required for BDNF-induced differentiation of neural precursor cells Biochem. Biophys Res Commun 301 1011–1015

    Google Scholar 

  • K Jin Y Zhu Y Sun XO Mao L Xie DA Greenberg (2002) ArticleTitleVascular endothelial growth factor (VEGF) stimulates neurogenesis in vitro and in vivo Proc Natl Acad Sci USA 99 11946–11950

    Google Scholar 

  • N Kee S Sivalingam R Boonstra JM Wojtowicz (2002) ArticleTitleThe utility of Ki-67 and BrdU as proliferative markers of adult neurogenesis J Neurosci Methods 115 97–105

    Google Scholar 

  • SE Kendall DE Goldhawk C Kubu PA Barker JM Verdi (2002) ArticleTitleExpression analysis of a novel p75(NTR) signaling protein, which regulates cell cycle progression and apoptosis Mech. Dev. 117 187–200

    Google Scholar 

  • R Lee P Kermani KK Teng BL Hempstead (2001) ArticleTitleRegulation of cell survival by secreted proneutrophins Science 294 1945–1948

    Google Scholar 

  • U Lendahl LB Zimmerman RD McKay (1990) ArticleTitleCNS stem cells express a new class of intermediate filament protein Cell 60 585–595

    Google Scholar 

  • DC Lie G Dziewczapolski AR Willhoite BK Kaspar CW Shults FH Gage (2002) ArticleTitleThe adult substantia nigra contains progenitor cells with neurogenic potential J Neurosci 22 6639–6649

    Google Scholar 

  • C Lois A Alvarez-Buylla (1994) ArticleTitleLong-distance neuronal migration in the adult mammalian brain Science 264 1145–1148

    Google Scholar 

  • C Lois JM Garcia-Verdugo A Alvarez-Buylla (1996) ArticleTitleChain migration of neuronal precursors Science 271 978–981

    Google Scholar 

  • N Lopez-Sanchez JM Frade (2002) ArticleTitleControl of the cell cycle by neurotrophins: lessons from the p75 neurotrophin receptor Histol Histopathol 17 1227–1237

    Google Scholar 

  • MB Luskin (1993) ArticleTitleRestricted proliferation and migration of postnatally generated neurons derived from the forebrain subventricular zone Neuron 11 173–189

    Google Scholar 

  • EA Markakis TD Palmer L Randolph-Moore P Rakic FH Gage (2004) ArticleTitleNovel neuronal phenotypes from neural progenitor cells J Neurosci 24 2886–2897

    Google Scholar 

  • CA Marshall SO Suzuki JE Goldman (2003) ArticleTitleGliogenic and neurogenic progenitors of the subventricular zone: who are they, where did they come from, and where are they going Glia 43 52–61

    Google Scholar 

  • AY Maslov TA Barone RJ Plunkett SC Pruitt (2004) ArticleTitleNeural stem cell detection, characterization, and age-related changes in the subventricular zone of mice J Neurosci 24 1726–1733

    Google Scholar 

  • CM Morshead BA Reynolds CG Craig MW McBurney WA Staines D Morassutti S Weiss D Kooy Particlevan der (1994) ArticleTitleNeural stem cells in the adult mammalian forebrain: a relatively quiescent subpopulation of subependymal cells Neuron 13 1071–1082

    Google Scholar 

  • A Nykjaer R Lee KK Teng P Jansen P Madsen MS Nielsen C Jacobsen M Kliemannel E Schwarz TE Willnow BL Hempstead CM Petersen (2004) ArticleTitleSortilin is essential for proNGF-induced neuronal cell death Nature 427 843–848

    Google Scholar 

  • L O’Connor DC Huang LA O’Reilly A Strasser (2000) ArticleTitleApoptosis and cell division Curr Opin Cell Biol 12 257–263

    Google Scholar 

  • HJ Okano DW Pfaff RB Gibbs (1996) ArticleTitleExpression of EGFR-, p75NGFR-, and PSTAIR (cdc2)-like immunoreactivity by proliferating cells in the adult rat hippocampal formation and forebrain Dev Neurosci 18 199–209

    Google Scholar 

  • G Paxinos C Watson (1986) The rat brain in stereotaxic coordinates Academic Press San Diego

    Google Scholar 

  • V Pencea KD Bingaman SJ Wiegand MB Luskin (2001) ArticleTitleInfusion of brain-derived neurotrophic factor into the lateral ventricle of the adult rat leads to new neurons in the parenchyma of the striatum, septum, thalamus, and hypothalamus J Neurosci 21 6706–6717

    Google Scholar 

  • L Petreanu A Alvarez-Buylla (2002) ArticleTitleMaturation and death of adult-born olfactory bulb granule neurons: role of olfaction J Neurosci 22 6106–6113

    Google Scholar 

  • JF Powers M Shahsavari P Tsokas AS Tischler (1999) ArticleTitleNerve growth factor receptor signaling in proliferation of normal adult rat chromaffin cells Cell Tissue Res 295 21–32

    Google Scholar 

  • P Rakic (2002) ArticleTitleAdult neurogenesis in mammals: an identity crisis J Neurosci 22 614–618

    Google Scholar 

  • L Redmond S Hockfield MA Morabito (1996) ArticleTitleThe divergent homeobox gene PBX1 is expressed in the postnatal subventricular zone and interneurons of the olfactory bulb J Neurosci 16 2972–2982

    Google Scholar 

  • JT Rifkin VJ Todd LW Anderson F Lefcort (2000) ArticleTitleDynamic expression of neurotrophin receptors during sensory neuron genesis and differentiation Dev Biol 227 465–480

    Google Scholar 

  • AH Salehi PP Roux CJ Kubu C Zeindler A Bhakar LL Tannis JM Verdi PA Barker (2000) ArticleTitleNRAGE, a novel MAGE protein, interacts with the p75 neurotrophin receptor and facilitates nerve growth factor-dependent apoptosis Neuron 27 279–288

    Google Scholar 

  • R Schliebs S Rossne V Bigl (1996) ArticleTitleImmunolesion by 192IgG-saporin of rat basal forebrain cholinergic system: a useful tool to produce cortical cholinergic dysfunction Prog Brain Res 109 253–264

    Google Scholar 

  • KB Seroogy CM Gall DC Lee HI Kornblum (1995) ArticleTitleProliferative zones of postnatal rat brain express epidermal growth factor receptor mRNA Brain Res 670 157–164

    Google Scholar 

  • HJ Song CF Stevens FH Gage (2002) ArticleTitleNeural stem cells from adult hippocampus develop essential properties of functional CNS neurons Nat Neurosci 5 438–445

    Google Scholar 

  • DL Stemple DJ Anderson (1992) ArticleTitleIsolation of a stem cell for neurons and glia from the mammalian neural crest Cell 71 973–985

    Google Scholar 

  • JL Urdiales E Becker M Andrieu A Thomas J Jullien LA Grunsven Particlevan S Menut GI Evan D Martin-Zanca BB Rudkin (1998) ArticleTitleCell cycle phase-specific surface expression of nerve growth factor receptors TrkA and p75(NTR) J Neurosci 118 6767–6775

    Google Scholar 

  • FM Vaccarino ML Schwartz R Raballo J Nilsen J Rhee M Zhou T Doetschman JD Coffin JJ Wyland YT Hung (1999) ArticleTitleChanges in cerebral cortex size are governed by fibroblast growth factor during embryogenesis Nat Neurosci 2 246–253

    Google Scholar 

  • KC Wang JA Kim R Sivasankaran R Segal Z He (2002) ArticleTitlep75 interacts with the Nogo receptor as a co-receptor for Nogo, MAG and Omgp Nature 420 74–78

    Google Scholar 

  • S Weiss C Dunne J Hewson C Wohl M Wheatley AC Peterson BA Reynolds (1996) ArticleTitleMultipotent CNS stem cells are present in the adult mammalian spinal cord and ventricular neuroaxis J Neurosci 16 7599–7609

    Google Scholar 

  • JP Wu JS Kuo YL Liu SF Tzeng (2000) ArticleTitleTumor necrosis factor-alpha modulates the proliferation of neural progenitors in the subventricular/ventricular zone of adult rat brain Neurosci Lett 292 203–206

    Google Scholar 

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Authors and Affiliations

  1. DIMORFIPA, University of Bologna, via Tolara di Sopra 50, 40064, Ozzano dell’ Emilia, Italy

    A. Giuliani, G. D’Intino, M. Paradisi, L. Giardino & L. Calzà

  2. Pathophysiology Center for the Nervous System, Hesperia Hospital, Modena, Italy

    L. Giardino & L. Calzà

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  1. A. Giuliani
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  2. G. D’Intino
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  3. M. Paradisi
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  4. L. Giardino
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  5. L. Calzà
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Giuliani, A., D’Intino, G., Paradisi, M. et al. p75NTR-Immunoreactivity in the subventricular zone of adult male rats: Expression by cycling cells. J Mol Hist 35, 749–758 (2004). https://doi.org/10.1007/s10735-004-9609-2

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  • DOI: https://doi.org/10.1007/s10735-004-9609-2

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