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Ectopic expression of N-acetylglucosamine 6-O-sulfotransferase 2 in chemotherapy-resistant ovarian adenocarcinomas

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Abstract

Mucinous and clear cell adenocarcinomas are the major histological types of ovarian epithelial cancer and are associated with a poor prognosis due to their resistance to chemotherapy. A novel tumor marker specific for ovarian mucinous and clear cell adenocarcinomas would be helpful for overcoming these serious diseases. We showed previously by enzymological characterization and RT-PCR that colonic mucinous adenocarcinoma tissues ectopically express GlcNAc6ST-2, a member of the carbohydrate 6-O-sulfotransferase family (Seko, A. et al. (2002) Glycobiology 12, 379–388). Here, we prepared a GlcNAc6ST-2-specific polyclonal antibody for immunohistochemical analysis and found that GlcNAc6ST-2 is ectopically expressed by not only colonic mucinous adenocarcinomas but also ovarian mucinous, clear cell and papillary serous adenocarcinomas. In contrast, solid serous adenocarcinomas, endometrioid adenocarcinomas, and mucinous adenomas expressed GlcNAc6ST-2 much less frequently or not at all. RT-PCR analysis confirmed that GlcNAc6ST-2 transcripts are expressed in ovarian mucinous adenocarcinomas but not in mucinous adenomas. In addition, immunohistochemical analysis using sulfated glycan-specific monoclonal antibodies showed that ovarian adenocarcinoma cells express GlcNAc 6-O-sulfated glycans, including an L-selectin ligand and its related glycans. These results indicate that GlcNAc6ST-2 would be a novel tumor antigen that is specifically expressed in ovarian mucinous, clear cell and papillary serous adenocarcinomas.

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Abbreviations

Gal:

galactose

GalNAc:

N-acetylgalactosamine

GlcNAc:

N-acetylglucosamine

HRP:

horseradish peroxidase

LacNAc:

N-acetyllactosamine

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Authors and Affiliations

  1. Hanno Discovery Center, TAIHCO Pharmaceutical Co. Ltd., Saitama, Japan

    Akira Kanoh

  2. Innovative Research Initiatives, Tokyo Institute of Technology, Yokohama

    Akira Seko, Hiroko Ideo & Katsuko Yamashita

  3. CREST, Japan Science and Technology Agency, Japan

    Akira Seko, Hiroko Ideo & Katsuko Yamashita

  4. National Institute of Radiological Sciences, Chiba, Japan

    Midori Yoshida

  5. Department of Pathology, Kagoshima University, Kagoshima, Japan

    Mitsuharu Nomoto & Suguru Yonezawa

  6. Department of Gynecology, Kyoundo Hospital, Tokyo, Japan

    Masaru Sakamoto

  7. Department of Molecular Pathology, Aichi Cancer Center, Nagoya, Japan

    Reiji Kannagi

Authors
  1. Akira Kanoh
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  2. Akira Seko
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  3. Hiroko Ideo
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  4. Midori Yoshida
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  5. Mitsuharu Nomoto
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  6. Suguru Yonezawa
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  7. Masaru Sakamoto
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  8. Reiji Kannagi
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  9. Katsuko Yamashita
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Correspondence to Katsuko Yamashita.

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Kanoh, A., Seko, A., Ideo, H. et al. Ectopic expression of N-acetylglucosamine 6-O-sulfotransferase 2 in chemotherapy-resistant ovarian adenocarcinomas. Glycoconj J 23, 453–460 (2006). https://doi.org/10.1007/s10719-006-6979-6

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  • DOI: https://doi.org/10.1007/s10719-006-6979-6

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