Abstract
The fossa (Cryptoprocta ferox) is one of eight carnivore species endemic to Madagascar. This species lives in the forests of Madagascar and is classified as vulnerable by the IUCN due to the loss and fragmentation of its natural habitat and the hunting pressure of local people. Twenty-six nuclear microsatellite loci were isolated from genomic DNA derived from a free-ranging fossa from Betampona, Madagascar. Population genetic parameters were estimated on 28 individuals from nine European zoos establishing a baseline to study the genetic health and diversity of captive fossas to support the European Breeding and Conservation Program (EEP). The marker suite averaged 4.4 alleles per locus with an average polymorphic information content of 0.478.
This is a preview of subscription content, log in via an institution to check access.
References
Albignac R (1972) The carnivora of Madagascar. In: Battistini R, Richard-Vinard G (eds) Biogeography and ecology in Madagascar. W. Junk, The Hague, pp 667–682
Bennett E (1833) Notice of a mammiferous animal from Madagascar, constituting a new form among the viverridous carnivora. Trans Zool Soc Lond 1:137–140
Dieringer D, Schlötterer C (2003) Microsatellite analyzer (MSA): a platform independent analysis tool for large microsatellite data sets. Mol Ecol Notes 3:167–169. doi:10.1046/j.1471-8286.2003.00351.x
Goudet J (1995) FSTAT, a computer program to test F-statistics. J Hered 86:85–486
Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Updated from Goudet (1995)
Hillis DM, Mable BK, Larson A, Davis SK, Zimmer EA (1996) Nucleic acids IV: sequencing and cloning. In: Hillis DM, Moritz C, Zimmer EA (eds) Molecular systematics, 2nd edn. Sinauer Associates Inc., Sunderland, pp 321–381
IUCN (2008) 2008 IUCN Red List of Threatened Species. www.iucnredlist.org. Downloaded on 08 October 2008
Kandpal RP, Kandpal G, Weissman SM (1994) Construction of libraries enriched for sequence repeats and jumping clones, and hybridization selection for region-species markers. Proc Natl Acad Sci USA 91:88–92. doi:10.1073/pnas.91.1.88
Marshall TC, Slate J, Kruuk LEB, Pemberton JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7(5):639–655. doi:10.1046/j.1365-294x.1998.00374.x
Moraga-Amador D, Farmerie BA, Brazeau D, Clark G (2001) “Tools for developing molecular markers. In: Interdisciplinary center for biotechnology research laboratory manual. The University of Florida Gainesville, Florida, pp 11–65
Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecuminicisms. J Hered 86:248–249
Sambrook J, Fritch EF, Maniatus T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Press, New York
Slate J, Marshall TC, Pemberton JM (2000) A retrospective assessment of the accuracy of the paternity inference program CERVUS. Mol Ecol 9(6):801–808. doi:10.1046/j.1365-294x.2000.00930.x
Van Oosterhaut C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538. doi:10.1111/j.1471-8286.2004.00684.x
Wemmer C, Wozencraft WC (2001) Civets, genets and mongooses. In: Macdonald D (ed) The new encyclopedia of mammals. Oxford University Press, Oxford, pp 122–131
Acknowledgments
The authors wish to acknowledge the generosity of Bill and Berniece Grewcock for their support of student interns. This research was also supported by grants from the Ahmanson Foundation, which have provided the laboratory with three ABI automated DNA sequencers. We graciously thank the Theodore F. and Claire M. Hubbard Family Foundation for their support of the Henry Doorly Zoo/Madagascar Biodiversity and Biogeography Project. We thank the German Academic Exchange Service (DAAD) and the Fossa Fund (Zoo Duisburg) which supported the research stays of the author BRV. This project would not have been possible without the support of the zoos of Amsterdam (NL), Berlin (D), Bratislava (SL), Chemnitz (D), Colchester (UK), Duisburg (D), Frankfurt (D), Olomouc (CZ), Omaha (NE, USA) and Ustí nad Labem (CZ).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Vogler, B.R., Bailey, C.A., Shore, G.D. et al. Characterization of 26 microsatellite marker loci in the fossa (Cryptoprocta ferox). Conserv Genet 10, 1449–1453 (2009). https://doi.org/10.1007/s10592-008-9758-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10592-008-9758-z