Abstract
Mast cell-mediated allergic inflammation is involved in many diseases such as asthma, sinusitis, and rheumatoid arthritis. Mast cells induce synthesis and production of pro-inflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 with immune regulatory properties. The formulated ethanol extract of Artemisia asiatica Nakai (DA-9601) has been reported to have antioxidative and anti-inflammatory activities. In this report, we investigated the effect of DA-9601 on the expression of pro-inflammatory cytokines by the activated human mast cell line HMC-1 and studied its possible mechanisms of action. DA-9601 dose-dependently decreased the gene expression and production of TNF-α, IL-1β, and IL-6 on phorbol 12-myristate 13-acetate (PMA)- and calcium ionophore A23187-stimulated HMC-1 cells. In addition, DA-9601 attenuated PMA- and A23187-induced activation of NF-κB as indicated by inhibition of degradation of IκBα, nuclear translocation of NF-κB, NF-κB/DNA binding, and NF-κB-dependent gene reporter assay. Our in vitro studies provide evidence that DA-9601 might contribute to the treatment of mast cell-derived allergic inflammatory diseases.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- ELISA:
-
enzyme-linked immunosorbent assay
- EMSA:
-
electrophoretic mobility shift assays
- HMC:
-
human mast cell line
- IL-1β:
-
interleukin (IL)-1β
- MTT:
-
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
- NF-κB:
-
nuclear factor-κB
- PBS:
-
phosphate-buffered saline
- PDTC:
-
pyrrolidine dithiocarbamate
- PMA:
-
phorbol 12-myristate 13-acetate
- RT-PCR:
-
reverse-transcriptase polymerase chain reaction
- TNF-α:
-
tumor necrosis factor
References
Azzolina A, Bongiovanni A, Lampiasi N. Substance P induces TNF-alpha and IL-6 production through NF kappa B in peritoneal mast cells. Biochim Biophys Acta. 2003;1643:75–83.
Beaven MA, Metzger H. Signal transduction by Fc receptors: the Fc epsilon RI case. Immunol Today. 1993;14:222–6.
Beaven MA, Rogers J, Moore JP, Hesketh TR, Smith GA, Metcalfe JC. The mechanism of the calcium signal and correlation with histamine release in 2H3 cells. J Biol Chem. 1984;259:7129–36.
Blackwell TS, Blackwell TR, Christman JW. Impaired activation of nuclear factor-kappaB in endotoxin-tolerant rats is associated with down-regulation of chemokine gene expression and inhibition of neutrophilic lung inflammation. J Immunol. 1997;158:5934–40.
Bradding P, Feather IH, Wilson S, et al. Immunolocalization of cytokines in the nasal mucosa of normal and perennial rhinitic subjects. The mast cell as a source of IL-4, IL-5, and IL-6 in human allergic mucosal inflammation. J Immunol. 1993;151:3853–65.
Burd PR, Rogers HW, Gordon JR, et al. Interleukin 3-dependent and -independent mast cells stimulated with IgE and antigen express multiple cytokines. J Exp Med. 1989;170:245–57.
Church MK, Levi-Schaffer F. The human mast cell. J Allergy Clin Immunol. 1997;99:155–60.
Hide I, Toriu N, Nuibe T, et al. Suppression of TNF-alpha secretion by azelastine in a rat mast (RBL-2H3) cell line: evidence for differential regulation of TNF-alpha release, transcription, and degranulation. J Immunol. 1997;159:2932–40.
Jeong HJ, Hong SH, Lee DJ, Park JH, Kim KS, Kim HM. Role of Ca(2+) on TNF-alpha and IL-6 secretion from RBL-2H3, mast cells. Cell Signal. 2002;14:633–9.
Kim JY, Kwon EY, Lee YS, Kim WB, Ro JY. Eupatilin blocks mediator release via tyrosine kinase inhibition in activated guinea pig lung mast cells. J Toxicol Environ Health A. 2005;68: 2063–80.
Kim SH, Sharma RP. Mercury-induced apoptosis and necrosis in murine macrophages: role of calcium-induced reactive oxygen species and p38, mitogen-activated protein kinase signaling. Toxicol Appl Pharmacol. 2004;196:47–57.
Kim SH, Shin TY. Amomum xanthiodes inhibits mast cell-mediated allergic reactions through the inhibition of histamine release and inflammatory cytokine production. Exp Biol Med (Maywood). 2005;230:681–7.
Kim SH, Bark H, Choi CH. Mercury induces multidrug resistance-associated protein gene through p38, mitogen-activated protein kinase. Toxicol Lett. 2005;155:143–50.
Kim SH, Jun CD, Suk K, et al. Gallic acid lnhibits histamine release and pro-inflammatory cytokine production in mast cells. Toxicol Sci. 2006;91:123–131.
Lee JS, Oh TY, Ahn BO, et al. Involvement of oxidative stress in experimentally induced reflux esophagitis and Barrett's esophagus: clue for the chemoprevention of esophageal carcinoma by antioxidants. Mutat Res. 2001;480–481:189–200.
Lin TJ, Issekutz TB, Marshall JS. Human mast cells transmigrate through human umbilical vein endothelial monolayers and selectively produce IL-8 in response to stromal cell-derived factor-1 alpha. J Immunol. 2000;165:211–20.
Marquardt DL, Walker LL. Dependence of mast cell IgE-mediated cytokine production on nuclear factor-kappaB activity. J Allergy Clin Immunol. 2000;105:500–5.
Mekori YA, Metcalfe DD. Mast cells in innate immunity. Immunol Rev. 2000;173:131–40.
Metcalfe DD, Kaliner M, Donlon MA. The mast cell. Crit Rev Immunol. 1981;3:23–74.
Mican JA, Arora N, Burd PR, Metcalfe DD. Passive cutaneous anaphylaxis in mouse skin is associated with local accumulation of interleukin-6, mRNA and immunoreactive interleukin-6 protein. J Allergy Clin Immunol. 1992;90:815–24.
Mullarkey MF, Leiferman KM, Peters MS, et al. Human cutaneous allergic late-phase response is inhibited by soluble IL-1 receptor. J Immunol. 1994;152:2033–41.
Oh TY, Lee JS, Ahn BO, et al. Oxidative damages are critical in pathogenesis of reflux esophagitis: implication of antioxidants in its treatment. Free Radic Biol Med. 2001a; 30:905–15.
Oh TY, Lee JS, Ahn BO, et al. Oxidative stress is more important than acid in the pathogenesis of reflux oesophagitis in rats. Gut. 2001b;49:364–71.
Plaut M, Pierce JH, Watson CJ, Hanley-Hyde J, Nordan RP, Paul WE. Mast cell lines produce lymphokines in response to cross-linkage of Fc epsilon RI or to calcium ionophores. Nature. 1989;339:64–7.
Ryu BK, Ahn BO, Oh TY, Kim SH, Kim WB, Lee EB. Studies on protective effect of DA-9601, Artemisia asiatica extract, on acetaminophen- and CCl4-induced liver damage in rats. Arch Pharm Res. 1998;21:508–13.
Sen R, Baltimore D. Multiple nuclear factors interact with the immunoglobulin enhancer sequences. Cell. 1986;46:705–16.
Seo HJ, Park KK, Han SS, et al. Inhibitory effects of the standardized extract (DA-9601) of Artemisia asiatica Nakai on phorbol ester-induced ornithine decarboxylase activity, papilloma formation, cyclooxygenase-2 expression, inducible nitric oxide synthase expression and nuclear transcription factor kappa B activation in mouse skin. Int J Cancer. 2002;100:456–62.
Siebenlist U, Franzoso G, Brown K. Structure, regulation and function of NF-kappa B. Annu Rev Cell Biol. 1994;10:405–55.
Theodorou V, Fioramonti J, Junien JL, Bueno L. Anaphylactic colonic hypersecretion in cow's milk sensitized guinea-pigs depends upon release of interleukin-1, prostaglandins and mast cell degranulation. Aliment Pharmacol Ther. 1994;8:301–7.
Walsh LJ, Trinchieri G, Waldorf HA, Whitaker D, Murphy GF. Human dermal mast cells contain and release tumor necrosis factor alpha, which induces endothelial leukocyte adhesion molecule 1. Proc Natl Acad Sci USA. 1991;88:4220–4.
Yang EK, Kim, InKyeom. Pulse exposure to ethanol augments vascular contractility through stress response. Korean J Physiol Pharmacol. 2001;5:47–53.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lee, S., Park, HH., Son, HY. et al. DA-9601 inhibits activation of the human mast cell line HMC-1 through inhibition of NF-κB. Cell Biol Toxicol 23, 105–112 (2007). https://doi.org/10.1007/s10565-006-0103-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10565-006-0103-3