Skip to main content
SpringerLink
Log in

Tissue cryobanking for conservation programs: effect of tissue type and storage time after death

  • Published:
Cell and Tissue Banking Aims and scope Submit manuscript

Abstract

In this study, we investigated the temporal post-mortem limits, within which there will be guarantees of obtaining living cells from several tissues of sheep and cattle and the effect of vitrification on the ability of cells from tissue stored at different times. Muscle tissue and auricular cartilage were stored at 4°C for 5, 48, 72, 96 and 216 h post-mortem (hpm). Tissue samples were sorted into two groups: one group was in vitro cultured immediately after storage and the other was vitrified after storage and then in vitro cultured. In cattle and sheep, no differences in subconfluence rates were observed between the two experimental groups. At the same time, no significant differences were observed in the number of days required in culture to reach confluence between non-vitrified and vitrified groups when tissues were stored at 4°C for different times. In sheep, while the population doubling times (PDT) were similar in cartilage cells from vitrified and non-vitrified tissues and stored at 4°C for 5 and 216 hpm, PDT of muscle cells were longer in 216 hpm stored groups than in 5 hpm stored groups. In bovine, although the PDT of muscle cells were similar for 5 and 216 hpm and both vitrified and non-vitrified tissues and the PDT were longer in cartilage cells from vitrified than from non-vitrified tissues. In conclusion, although storage times and vitrification have different effects on tissues from cattle and sheep, this study showed that living cells could be obtained from all groups. Therefore, cartilage and muscle tissues can be stored at 4°C for 216 hpm and used for cyrobanking.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Akkoc T, Taskin C, Caputcu TA, Arat S, Bagis H (2011) The effect of solid surface vitrificaiton (SSV) versus classic vitrification technique on survive rate of in vitro produced bovine blastocysts. J Anim Vet Adv 10(22):2885–2891

    CAS  Google Scholar 

  • Andrabi SMH, Maxwell WMC (2007) A review of reproductive biotechnologies for conservation of endangered mammalian species. Anim Reprod Sci 99:223–243

    Article  PubMed  CAS  Google Scholar 

  • Arat S, Gibbons J, Rzucidlo SJ, Respess DS, Tumlin M, Stice SL (2002) In vitro development of bovine nuclear transfer embryos from clonal lines of transgenic adult and fetal fibroblast cells of the same genotype. Biol Reprod 66:1768–1774

    Article  PubMed  CAS  Google Scholar 

  • Arat S, Bagis H, Ergin F, Sagirkaya H, Mercan Odaman H, Dinnyes A (2004) Cold storage of tissue as source for donor cells does not reduce the in vitro development of bovine embryos following nuclear transfer. Reprod Fertil Dev 16(1,2):135

    Article  Google Scholar 

  • Arat S, Bagis H, Mercan Odaman H, Dinnyes A (2005) Cloned embryos can be produced using donor cells obtained from 72-hour cooled carcass. Reprod Fertil Dev 17:164

    Article  Google Scholar 

  • Arat S, Tas A, Akkoc T, Cetinkaya G, Bagis H, Sekmen S, Ates E, Soysal D (2009) Effect of growth factors on development of nuclear transfer embryos from cartilage cell of an Anatolian native cow. Reprod Domes Anim 44:93

    Google Scholar 

  • Arat S, Caputcu AT, Akkoc T, Pabuccuoglu S, Sagirkaya H, Cirit U, Nak Y, Koban E, Bagis H, Demir K, Nak D, Senunver A, Kilicaslan R, Tuna B, Cetinkaya G, Denizci M, Aslan O (2011) Using cell banks as a tool in conservation programmes of native domestic breeds: the production of the first cloned Anatolian grey cattle. Reprod Fertil Dev 23(8):1012–1023

    Article  PubMed  Google Scholar 

  • Asawa Y, Ogasawara T, Takahashi T, Yamaoka H, Nishizawa S, Matsudaira K, Mori Y, Takato T, Hoshi K (2009) Aptitude of auricular and nasoseptal chondrocytes cultured under a monolayer or three-dimensional condition for cartilage tissue engineering. Tissue Eng Part A 15:1109–1118

    Article  PubMed  CAS  Google Scholar 

  • Bagis H, Akkoc T, Tas A, Aktoprakligil D (2008) Cryogenic effect of antifreeze protein on transgenic mouse ovaries and the production of live offspring by orthotopic transplantation of cryopreserved mouse ovaries. Mol Reprod Dev 75:608–613

    Article  PubMed  CAS  Google Scholar 

  • Bagis H, Akkoc T, Taskin C, Arat S (2010) Comparison of different cryopreservation techniques: higher survival and implantation rate of frozen-thawed mouse pronuclear embryos in the presence of beta-mercaptoethanol in post-thaw culture. Reprod Domest Anim 45:332–337

    Article  Google Scholar 

  • Brem G, Kuhholzer B (2002) The recent history of somatic cloning in mammals. Cloning Stem Cells 4(1):57–63

    Article  PubMed  CAS  Google Scholar 

  • Cetinkaya G, Arat S (2011) Cryopreservation of cartilage cell and tissue for biobanking. Cyrobiology 63:292–297

    Article  CAS  Google Scholar 

  • Day JG, Stacey GN (2007a) Cryopreservation and freeze-drying protocols. In: Day JG, Stacey GN (eds) Long-term ex situ conservation of biological resources and the role of biological resource centers, 2nd edn. Humana Press, New Jersey, pp 1–14

    Google Scholar 

  • Day JG, Stacey GN (2007b) Cryopreservation and freeze-drying protocols. In: Sputtek A (ed) Cryopreservation of Red Blood Cells and Platelets, 2nd edn. Humana Press, New Jersey, pp 283–301

    Google Scholar 

  • Fahy GM, Wowk B, Wu J, Phan J, Rasch C, Chang A, Zendejas E (2004) Cryopreservation of organs by vitrification: perspectives and recent advances. Cryobiology 48:157–178

    Article  PubMed  CAS  Google Scholar 

  • Fröhlich M, Malicev E, Gorensek M, Knezevic M, Velikonja NK (2007) Evaluation of rabbit auricular chondrocyte isolation and growth parameters in cell culture. Cell Biol Int 31:620–625

    Article  PubMed  Google Scholar 

  • Gajda B, Katska-Ksiazkiewicz L, Rynska B, Bochenek M, Smorag Z (2007) Survival of bovine fibroblasts and cumulus cells after vitrification. Cryo Lett 28(4):271–279

    Google Scholar 

  • Gañán N, Sestelo A, Garde JJ, Martínez F, Vargas A, Sánchez I, Pérez-Aspa MJ, López-Bao JV, Palomares F, Gomendio M, Roldan ER (2010) Reproductive traits in captive and free-ranging males of the critically endangered Iberian lynx (Lynx pardinus). Reproduction 139:275–285

    Article  PubMed  Google Scholar 

  • Gibbons J, Arat S, Rzucidlo SJ, Waltenburg R, Respess DS, Venable AM, Stice SL (2002) Enhanced survivability of cloned calves derived from roscovitine-treated adult somatic cells. Biol Reprod 66:895–900

    Article  PubMed  CAS  Google Scholar 

  • Hay RJ (1992) Cell line preservation and characterization. In: Freshney RI (ed) Animal Cell Culture. Oxford University Press, New York, A Practical Approach, pp 95–148

    Google Scholar 

  • Ideta A, Hayama K, Urakawa M, Tsuchiya K, Aoyagi Y, Saeki K (2010) Comparison of early development in utero of cloned fetuses derived from bovine fetal fibroblasts at the G1 and G0/G1 phases. Anim Reprod Sci 119:191–197

    Article  PubMed  Google Scholar 

  • Leon-Quinto T, Simon MA, Cadenas R, Jones J, Martinez-Hernandez FJ, Moreno JM, Vargas A, Martinez F, Soria B (2009) Developing biological resource banks as a supporting tool for wildlife reproduction and conservation the Iberian lynx bank as a model for other endangered species. Anim Reprod Sci 112:347–361

    Article  PubMed  Google Scholar 

  • Loi P, Ptak G, Barboni B, Fulka J Jr, Cappai P, Clinton M (2001) Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells. Nat Biotech 19:962–964

    Article  CAS  Google Scholar 

  • Martin H, Bournique B, Sarsat JP, Albaladejo V, Lerche-Langand C (2000) Cryopreserved rat liver slices: a critical evaluation of cell viability, histological integrity, and drug metabolizing enzymes. Cryobiology 41:135–144

    Article  PubMed  CAS  Google Scholar 

  • McLaren A (2000) Cloning: pathways to a pluripotent future. Science 288:1775–1780

    Article  PubMed  CAS  Google Scholar 

  • Morgan SJ, Darling DC (1993) Animal cell culture, In: JM Graham, D Billington (eds), BIOS scientific publisher limited, UK, 161

  • Nel-Themaat L, Gómez MC, Damiani P, Wirtu G, Dresser BL, Bondioli KR, Lyons LA, Pope CE, Godke RA (2007) Isolation, culture and characterisation of somatic cells derived from semen and milk of endangered sheep and eland antelope. Reprod Fertil Dev 19:576–584

    Article  PubMed  CAS  Google Scholar 

  • Orief Y, Schultze-Masgau A, Dafopoulus K, Al-hasani S (2005) Vitrification: will it replace the conventional gamete cryopreservation techniques. Middle East Fertil Soc J 10(3):171–184

    Google Scholar 

  • Pegg DE, Wusteman MC, Wand L (2006) Cryopreservation of articular cartilage. Part 1: conventional cryopreservation methods. Cryobiology 52:335–346

    Article  PubMed  CAS  Google Scholar 

  • Prentice JR, Anzar M (2011) Cryopreservation of mammalian oocyte for conservation of animal genetics. Vet Med Int. doi:10.4061/2011/146405

    Google Scholar 

  • Ryder OA (2002) Cloning advances and challenges for conservation. Trends Biotechnol 20:231–232

    Article  PubMed  CAS  Google Scholar 

  • Saeed AM, Escriba MJ, Silvestre MA, Garcia-Ximenez F (2000) Vitrification and rapid-freezing of cumulus cells from rabbits and pigs. Theriogenology 54:1359–1371

    Article  PubMed  CAS  Google Scholar 

  • Shiga K, Fujita T, Hirose K, Sasae Y, Nagai T (1999) Production of calves by transfer of nuclei from cultured somatic cells obtained from Japanese black bulls. Theriogenology 52(3):527–535

    Article  PubMed  CAS  Google Scholar 

  • Shroeder AC, Johnston D, Epping JJ (1991) Reversal of post-mortem degeneration of mouse oocytes during meiotic maturation in vitro. J Exp Zool 258:240–245

    Article  Google Scholar 

  • Silvestre MA, Saeed AM, Escriba MJ, Garcia-Ximenez F (2000) Vitrification and rapid-freezing of cumulus cells from rabbits and pigs. Theriogenology 54(9):1359–1371

    Article  PubMed  Google Scholar 

  • Silvestre MA, Saeed AM, Escribá MJ, García-Ximénez F (2002) Vitrification and rapid freezing of rabbit fetal tissues and skin samples from rabbits and pigs. Theriogenology 58(1):69–76

    Article  PubMed  CAS  Google Scholar 

  • Silvestre MA, Saeed AM, Cervera RP, Escriba MJ, Garcia-Ximenez F (2003) Rabbit and pig ear skin sample cryobanking: effects of storage time and temperature of the whole ear extirpated immediately after death. Theriogenology 59:1469–1477

    Article  PubMed  CAS  Google Scholar 

  • Silvestre MA, Sanchez JP, Gomez EA (2004) Vitrification of goat, sheep, and cattle skin samples from whole ear extirpated after death and maintained at different storage times and temperatures. Cryobiology 49:221–229

    Article  PubMed  CAS  Google Scholar 

  • Smith LC, Bordignon V, Babkine M, Fecteau G, Keefer C (2000) Benetif and problems with cloning animals. Can Vet J 4:919–924

    Google Scholar 

  • Stolzing A, Scutt A (2006) Age-related impairment of mesenchymal progenitor cell function. Aging Cell 5:213–224

    Article  PubMed  CAS  Google Scholar 

  • Vajta G, Nagy ZP, Cobo A, Conceicao J, Yovich J (2009) Vitrification in assisted reproduction: myths, mistakes, disbeliefs and confusion. Biomed Online 19:1–7

    Article  Google Scholar 

  • Wells DNA, Misica PM, Tervit HR, Vivanco WH (1998) Adult somatic cell NT in used to preserve the last surviving cow of Enderby Island cattle breed. Reprod Fertil Dev 10:369–378

    Article  PubMed  CAS  Google Scholar 

  • Wildt DE, Wemmer C (1999) Sex and wildlife: the role of reproductive science in conservation. Biodivers Conserv 8:965–976

    Article  Google Scholar 

  • Wusteman M, Robinson M, Pegg D (2004) Vitrification of large tissues with dielectric warming: biological problems and some approaches to their solution. Cryobiology 48:179

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We would like to thank Dr. Digdem Aktoprakligil Aksu for manuscript review; Fatih Karakaya, Erman Ates and Ozlem Celasin for technical assistance. The manuscript has been edited by native speaker Dr. Anita L. Akkas, who has PhD degree in English Literature and MA degree in Linguistics Engineering and Science. This research was funded by the Scientific and Technological Research Council of Turkey (TUBITAK) (Project no. KAMAG-106G005).

Author information

Authors and Affiliations

  1. TUBITAK MRC Genetic Engineering and Biotechnology Institute, Gebze, Kocaeli, Turkey

    Arzu Tas Caputcu, Tolga Akkoc & Gaye Cetinkaya

  2. Department of Agricultural Biotechnology, Namik Kemal University Agriculture Faculty, Tekirdag, Turkey

    Sezen Arat

Authors
  1. Arzu Tas Caputcu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tolga Akkoc
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gaye Cetinkaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sezen Arat
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sezen Arat.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Caputcu, A.T., Akkoc, T., Cetinkaya, G. et al. Tissue cryobanking for conservation programs: effect of tissue type and storage time after death. Cell Tissue Bank 14, 1–10 (2013). https://doi.org/10.1007/s10561-012-9292-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10561-012-9292-6

Keywords

Search

Navigation