Abstract
A sensitive and low-cost microfluidic integrated biosensor is developed based on the localized surface plasmon resonance (LSPR) properties of gold nanoparticles, which allows label-free monitoring of biomolecular interactions in real-time. A novel quadrant detection scheme is introduced which continuously measures the change of the light transmitted through the nanoparticle-coated sensor surface. Using a green light emitting diode (LED) as a light source in combination with the quadrant detection scheme, a resolution of 10−4 in refractive index units (RIU) is determined. This performance is comparable to conventional LSPR-based biosensors. The biological sensing is demonstrated using an antigen/antibody (biotin/anti-biotin) system with an optimized gold nanoparticle film. The immobilization of biotin on a thiol-based self-assembled monolayer (SAM) and the subsequent affinity binding of anti-biotin are quantitatively detected by the microfluidic integrated biosensor and a detection limit of 270 ng/mL of anti-biotin was achieved. The microfluidic chip is capable of transporting a precise amount of biological samples to the detection areas to achieve highly sensitive and specific biosensing with decreased reaction time and less reagent consumption. The obtained results are compared with those measured by a surface plasmon resonance (SPR)-based Biacore system for the same binding event. This study demonstrates the feasibility of the integration of LSPR-based biosensing with microfluidic technologies, resulting in a low-cost and portable biosensor candidate compared to the larger and more expensive commercial instruments.
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References
K. Bonroy, F. Frederix, G. Reekmans, E. Dewolf, R. De Palma, G. Borghs, P. Declerck, B. Goddeeris, J. Immunol. Methods 312, 167–181 (2006). doi:10.1016/j.jim.2006.03.007
A. De Leebeeck, L.K. Kumar, V. de Lange, D. Sinton, R. Gordon, A.G. Brolo, Anal. Chem 79, 4094–4100 (2007). doi:10.1021/ac070001a
R.M. Deirdre, R.H. Mark, Science 297, 1197–1198 (2002). doi:10.1126/science.297.5584.1197
R. De Palma, G. Reekmans, W. Laureyn, G. Borghs, G. Maes, Anal. Chem 79, 7540–7548 (2007). doi:10.1021/ac0713407
T. Endo, S. Yamamura, N. Nagatani, Y. Morita, Y. Takamura, E. Tamiya, Sci. Technol. Adv. Mater 6, 491–500 (2005). doi:10.1016/j.stam.2005.03.019
D. Erickson, S. Mandal, A.H.J. Yang, B. Cordovez, Microfluid. Nanofluid 4, 33–52 (2008). doi:10.1007/s10404-007-0198-8
F. Frederix, J.M. Friedt, K.H. Choi, W. Laureyn, A. Campitelli, D. Mondelaers, G. Maes, G. Borghs, Anal. Chem 75, 6894–6900 (2003). doi:10.1021/ac0346609
K. Fujiwara, H. Watarai, H. Itoh, E. Nakahama, N. Ogawa, Anal. Bioanal. Chem 386, 639–644 (2006). doi:10.1007/s00216-006-0559-2
A.J. Haes, R.P. Van Duyne, J. Am. Chem. Soc 124, 10596–10604 (2002). doi:10.1021/ja020393x
X.D. Hoa, A.G. Kirk, M. Tabrizian, Biosens. Bioelectron 23, 151–160 (2007). doi:10.1016/j.bios.2007.07.001
E. Hutter, M.P. Pileni, J. Phys. Chem. B 107, 6497–6499 (2003). doi:10.1021/jp0342834
Y. Iwasaki, T. Horiuchi, O. Niwa, Anal. Chem 73, 1595–1598 (2001). doi:10.1021/ac0012851
T. Kalkbrenner, U. Hakanson, V. Sandoghdar, Nano Lett 4, 2309–2314 (2004). doi:10.1021/nl048694n
C.K. Kim, R.R. Kalluru, J.P. Singh, A. Fortner, J. Griffin, G.K. Darbha, P.C. Ray, Nanotechnology 17, 3085–3093 (2006). doi:10.1088/0957-4484/17/13/001
S.J. Kim, K.V. Gobi, H. Iwasaka, H. Tanaka, N. Miura, Biosens. Bioelectron 23, 701–707 (2007). doi:10.1016/j.bios.2007.08.010
M. Lahav, A. Vaskevich, I. Rubinstein, Langmuir 20, 7365–7367 (2004). doi:10.1021/la0489054
E.M. Larsson, J. Alegret, M. Käll, D.S. Sutherland, Nano Lett 7, 1256–1263 (2007). doi:10.1021/nl0701612
G.L. Liu, J. Kim, Y. Lu, P.L. Lee, Nat. Mater 5, 27–32 (2006). doi:10.1038/nmat1528
Y.Q. Luo, Y. Fang, R.N. Zare, Lab Chip 8, 694–700 (2008). doi:10.1039/b800606g
X.L. Mao, J.R. Waldeisen, B.K. Juluri, T.J. Huang, Lab Chip 7, 1303–1308 (2007). doi:10.1039/b708863a
K. Mitsui, Y. Handa, K. Kajikawa, Appl. Phys. Lett 85, 4231–4233 (2004). doi:10.1063/1.1812583
V. Nanduri, A.K. Bhunia, S.I. Tu, G.C. Paoli, J.D. Brewster, Biosens. Bioelectron 23, 248–252 (2007). doi:10.1016/j.bios.2007.04.007
N. Nath, A. Chilkoti, Anal. Chem 76, 5370–5378 (2004). doi:10.1021/ac049741z
S. Peeters, T. Stakenborg, G. Reekmans, W. Laureyn, L. Lagae, A. Van Aerschot, M. Van Ranst, Biosens. Bioelectron 24, 72–77 (2008). doi:10.1016/j.bios.2008.03.012
S.D. Petra, M. Andreas, Nat. Rev. Drug Discov 5, 210–218 (2006). doi:10.1038/nrd1985
W. Rechberger, A. Hohenau, A. Leitner, J.R. Krenn, B. Lamprecht, F.R. Aussenegg, Opt. Commun 220, 137–141 (2003). doi:10.1016/S0030-4018(03)01357-9
J.C. Riboh, A.J. Haes, A.D. McLFarland, C.R. Yonzon, R.P. Van Duyne, J. Phys. Chem. B 107, 1772–1780 (2003). doi:10.1021/jp022130v
P.E. Sheehan, L.J. Whiteman, Nano Lett 5, 803–807 (2005). doi:10.1021/nl050298x
D.A. Stuart, A.J. Haes, C.R. Yonzon, E.M. Hicks, R.P. Van Duyne, I.E.E. Proc. Nanobiotechnol 152, 13–32 (2005). doi:10.1049/ip-nbt:20045012
S. Tanev, V.V. Tuchin, P. Paddon, Laser Phys. Lett 3, 594–598 (2006). doi:10.1002/lapl.200610052
B.H. Weigl, R.L. Bardell, C.R. Cabrera, Adv. Drug Res 55, 349–377 (2003). doi:10.1016/S0169-409X(02)00223-5
K.A. Willets, R.P. Van Duyne, Annu. Rev. Phys. Chem 58, 267–297 (2007). doi:10.1146/annurev.physchem.58.032806.104607
J. Ye, K. Bonroy, D. Nelis, F. Frederix, J. D’Haen, G. Maes, G. Borghs, Colloids Surf. A Physicochem. Eng. Asp 321, 313–317 (2008). doi:10.1016/j.colsurfa.2008.01.028
P.K. Yuen, G.S. Li, Y.J. Bao, U.R. Muller, Lab Chip 3, 46–50 (2003). doi:10.1039/b210274a
H.Q. Zhang, S. Boussaad, N.J. Tao, Rev. Sci. Instrum. 74, 150–153 (2003). doi:10.1063/1.1523649
Z.W. Zou, J.H. Kai, M.J. Rust, J. Han, C.H. Ahn, Sens. Actua A 136, 518–526 (2007). doi:10.1016/j.sna.2006.12.006
Acknowledgements
The research described in this paper is performed in the frame of the smartHEALTH Integrated Project, which is partially funded by the European Commission under contract No. 016817. The authors would also like to thank the Microfluidic Chip-shop Ltd. (Germany) and the Institut für Mikrotechnik Mainz Ltd. (IMM, Germany) for their help and for the fabrication of the microfluidic chip.
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Huang, C., Bonroy, K., Reekmans, G. et al. Localized surface plasmon resonance biosensor integrated with microfluidic chip. Biomed Microdevices 11, 893–901 (2009). https://doi.org/10.1007/s10544-009-9306-8
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DOI: https://doi.org/10.1007/s10544-009-9306-8