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Purification and some properties of an extracellular ribonuclease with antiviral activity against tobacco mosaic virus from Bacillus cereus

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Abstract

A new ribonuclease (RNase) with tobacco mosaic virus inhibition was isolated and purified from Bacillus cereus ZH14 through ammonium sulfate precipitation, ultrafiltration, ion-exchange chromatography of DEAE-Sephadex A-50 column, and gel chromatography of Sephacryl S-200HR column. The enzyme was purified approximately 134-fold with a recovery of 9.2%. The RNase had an MW of 75.6 kDa in SDS-PAGE, which differed from RNases reported previously. The inhibitory activity of the RNase in the purification process against tobacco mosaic virus was tested, and the percentage inhibition of the purified RNase (48 U/ml) reached 90%. The protein could tolerate 90°C and pH 4.0.

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Acknowledgments

The authors are grateful to the China Center of Industrial Culture Collection and the China National Research Institute of Food and Fermentation Industries for their financial support.

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Authors and Affiliations

  1. College of Food Science and Nutritional Engineering, China Agricultural University, 100083, Beijing, China

    Wen-Wen Zhou & Tian-Gui Niu

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  1. Wen-Wen Zhou
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  2. Tian-Gui Niu
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Correspondence to Wen-Wen Zhou.

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Zhou, WW., Niu, TG. Purification and some properties of an extracellular ribonuclease with antiviral activity against tobacco mosaic virus from Bacillus cereus . Biotechnol Lett 31, 101–105 (2009). https://doi.org/10.1007/s10529-008-9831-1

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  • DOI: https://doi.org/10.1007/s10529-008-9831-1

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