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Highly Unsaturated Fatty Acid Synthesis in Atlantic Salmon: Characterization of ELOVL5- and ELOVL2-like Elongases

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Abstract

Fish species vary in their capacity to biosynthesize the n-3 long-chain polyunsaturated fatty acids (LC-PUFA), eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids that are crucial to the health of higher vertebrates. The synthesis of LC-PUFA involves enzyme-mediated fatty acyl desaturation and elongation. Previously, a complementary DNA (cDNA) for an elongase, now termed elovl5a, had been cloned from Atlantic salmon. Here, we report on the cloning of two new elongase cDNAs: a second elovl5b elongase, corresponding to a 294-amino-acid (aa) protein, and an elovl2-like elongase, coding for a 287-aa protein, characterized for the first time in a nonmammalian vertebrate. Heterologous expression in yeast showed that the salmon Elovl5b elongated C18 and C20 PUFA, with low activity towards C22, while Elovl2 elongated C20 and C22 PUFA with lower activity towards C18 PUFA. All three transcripts showed predominant expression in the intestine and liver, followed by the brain. Elongase expression showed differential nutritional regulation. Levels of elovl5b and particularly of elovl2, but not of elovl5a, transcripts were significantly increased in liver of salmon fed vegetable oils (VO) compared to fish fed fish oil (FO). Intestinal expression showed a similar pattern. Phylogenetic comparisons indicate that, in contrast to salmon and zebra fish, Acanthopterygian fish species lack elovl2 which is consistent with their negligible ability to biosynthesize LC-PUFA and to adapt to VO dietary inclusion, compared to predominantly freshwater salmonids. Thus, the presence of elovl2 in salmon explains the ability of this species to biosynthesize LC-PUFA and may provide a biotechnological tool to produce enhanced levels of LC-PUFA, particularly DHA, in transgenic organisms.

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Abbreviations

aa:

amino acid

ARA:

arachidonic acid (20:4n-6)

DHA:

docosahexaenoic acid (22:6n-3)

EPA:

eicosapentaenoic acid (20:5n-3)

ER:

endoplasmic reticulum

FA:

fatty acid

FO:

fish oil

LC-PUFA:

Long chain polyunsaturated fatty acids (carbon chain length ≥ C20 with ≥3 double bonds)

LO:

linseed oil

ORF:

open reading frame

PUFA:

polyunsaturated fatty acids

qPCR:

quantitative (real-time) polymerase chain reaction

RACE:

rapid amplification of cDNA ends

RO:

rapeseed oil

SO:

soybean oil

UTR:

untranslated region

VO:

vegetable oil

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Acknowledgements

This work and XZ were supported by the Biotechnology and Biological Sciences Research Council Responsive Mode Grant, BB/C51237X/1 (Transcriptional control of polyunsaturated fatty acid synthesis in fish). SM was supported by “Fundação para a Ciência e a Tecnologia”, Portugal (grant SFRH/BPD/34247/2006) and OM by the postdoctoral research program of the Fundación Española para la Ciencia y la Tecnología (Ministerio de Educación y Ciencia).

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  1. Institute of Aquaculture, University of Stirling, Stirling, FK9 4LA, Scotland, UK

    Sofia Morais, Oscar Monroig, Xiaozhong Zheng, Michael J. Leaver & Douglas R. Tocher

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  1. Sofia Morais
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  2. Oscar Monroig
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  3. Xiaozhong Zheng
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  4. Michael J. Leaver
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  5. Douglas R. Tocher
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Correspondence to Sofia Morais.

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Morais, S., Monroig, O., Zheng, X. et al. Highly Unsaturated Fatty Acid Synthesis in Atlantic Salmon: Characterization of ELOVL5- and ELOVL2-like Elongases. Mar Biotechnol 11, 627–639 (2009). https://doi.org/10.1007/s10126-009-9179-0

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