Abstract
Cloacal anomalies exhibit a wide variety of morphologic types and accompanying clinical severity. The association of malformations of the cloaca with partial, complete, or conjoined twinning has been appreciated for some time, but, with the advent of prenatal ultrasound technology, appears to occur with a greater frequency than once thought. This observation has important implications for pathogenesis. We present 2 representative cases, a 19-week-old female fetus with duplication of several caudal structures and a 21-week-old male fetus with cloacal exstrophy variant and demised co-twin with lower abdominal wall defect, extruded intestinal tract, absent external genitalia, and imperforate anus. These findings and previously published theories suggest that certain models of monozygotic twinning may apply to the pathogenesis of cloacal anomalies. Specifically, the partial or complete duplication of the organizing center within a single embryonic disc may increase the risk of mesodermal insufficiency and thus account for the failure of complete development of the cloacal membrane and consequent exstrophy or other aberration.
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References
Marshall VF, Muecke EC. Variations in exstrophy of the bladder. J Urol 1962;88:766–796
Muecke E. The role of the cloacal membrane in exstrophy—the first successful experimental study. J Urol 1964;92:659–667
Beckwith JB. The congenitally malformed VII. Exstrophy of the bladder and cloacal exstrophy. Northwest Med 1966;65:407–410
Beckwith JB. Macroglossia, omphalocele, adrenal cytomegaly, gigantism and hyperplastic visceromegaly. Birth Defects Orig Art Ser 1969;5:188–196
McLaughlin JF, Marks WM, Jones G. Prospective management of exstrophy of the cloaca and myelocystocele following prenatal ultrasound recognition of neural tube defects in identical twins. Am J Med Genet 1984;19:721–7
Ornoy A, Navot D, Menashi M, Laufer N, Chemke J. Asymmetry and discordance for congenital anomalies in conjoined twins: a report of six cases. Teratology 1980;22:145–154
Redman J, Seibert J, Page B. Cloacal exstrophy in identical twins. Urology 1981;17:73–74
Austin PF, Homsy YL, Gearhart JP, Porter K, Guidi C, Madsen K, et al. The prenatal diagnosis of cloacal exstrophy. J Urol 1998;160:1179–1181
Kutzner DK, Wilson WG, Hogge WA. OEIS complex (cloacal exstrophy): prenatal diagnosis in the second trimester. Prenat Diagn 1988;8:247–253
Landy HJ, Keith LG. The vanishing twin: a review. Hum Reprod Update 1998;4:177–183
Meizner I, Levy A, Barnhard Y. Cloacal exstrophy sequence: an exceptional ultrasound diagnosis. Obstet Gynecol 1995;86:446–450
Casale P, Grady RW, Waldhausen JH, Joyner BD, Wright J, Mitchell ME. Cloacal exstrophy variants. Can blighted conjoined twinning play a role? J Urol 2004;172:1103–1107
Chitrit Y, Zorn B, Filidori M, Robert E, Chasseray JE. Cloacal exstrophy in monozygotic twins detected through antenatal ultrasound scanning. J Clin Ultrasound 1993;21:339–342
Lee DH, Cottrell JR, Sanders RC, Meyers CM, Wulfsberg EA, Sun C-CJ. OEIS complex (omphalocele-exstrophy-imperforate anus-spinal defects) in monozygotic twins. Am J Med Genet 1999;84:29–33
Azmy AF. Complete duplication of the hindgut and lower urinary tract with diphallus. J Pediatr Surg 1990;25:647–649
Dominguez R, Rott J, Castillo M, Pittaluga RR, Corriere JN Jr. Caudal duplication syndrome. Am J Dis Child 1993;147:1048–1052
Ravitch MM, Scott WW. Duplication of the entire colon, bladder, and urethra. Surgery 1953;34:843–858
Evans JA, Chudley AE. Tibial agenesis, femoral duplication, and caudal midline anomalies. Am J Med Genet 1999;85:13–19
La Torre R, Fusaro P, Anceschi MM, Montanino-Oliva M, Modesto S, Cosmi EV. Unusual case of caudal duplication (dipygus). J Clin Ultrasound 1998;26:163–165
Ulman I, Avanoglu A, Erdener A, Sahin AH, Gokdemir A. Evaluation of the lower urinary tract function in caudal duplication (dipygus) anomaly. J Pediatr Surg 1996;31:1680–1681
Bannykh SI, Bannykh GI, Mannino FL, Jones KL, Hansen L, Benirschke K, et al. Partial caudal duplication in a newborn associated with meningomyelocele and complex heart anomaly. Teratology 2001;63:94–99
Gastol P, Baka-Jakubiak M, Skobejko-Wlodarska L, Szymkiewicz C. Complete duplication of the bladder, urethra, vagina, and uterus in girls. Urology 2000;55:578–581
Gyftopoulos K, Wolffenbuttel KP, Nijman RJM. Clinical and embryologic aspects of penile duplication and associated anomalies. Urology 2002;60:675–679
Jimenez SG, Oliver MR, Stokes KB, Morreau PN, Chow CW. Case report: colonic duplication: a rare cause of obstruction. J Gastroenterol Hepatol 1999;14:889–892
Salman AB. Cloacal duplication. J Pediatr Surg 1996;31:1587–1588
Payne CE, Deshon GE Jr, Kroll JD, Sumfest J. Colonic duplication: an unusual cause of enterovesical fistula. Urology 1995;46:726–728
Hunter TB, Tonkin IL. Complete duplication of the colon in assoication with urethral duplication. Gastrointest Radiol 1979;4:93–95
Currie AB, Hemalatha VH, Doraiswamy NV. Duplications of the alimentary tract. J R Coll Surg Edinb 1978;23:347–354
Potter E. Pathology of the Fetus and Infant, 2nd ed. Chicago: Yearbook Medical Publishers, 1969
Soper R. Tubular duplication of the colon and distal ileum: case report and discussion. Surgery 1968;63:998–1000
Collins D. A study of 50,000 specimens of the human vermiform appendix. Surg Gynecol Obstet 1955;101:437–445
Nielsen OH, Nielsen R, Parvinen T. Duplicate exstrophy of the bladder. Ann Chir Gynaecol 1980;69:32–36
Muller F, O’Rahilly R. The primitive streak, the caudal eminence and related structures in staged human embryos. Cells Tissues Organs 2004;177:2–20
Griffith CM, Wiley MJ, Sanders EJ. The vertebrate tail bud: three germ layers from one tissue. Anat Embryol 1992;185:101–113
Keppler-Noreuil KM. OEIS complex (omphalocele-exstrophy-imperforate anus-spinal defects): a review of 14 cases. Am J Med Genet 2001;99:271–279
Pourquie O. The chick embryo: a leading model in somatogenesis studies. Mech Dev 2004;121:1069–1079
Alles AJ, Sulik KK. A review of caudal dysgenesis and its pathogenesis as illustrated in an animal model. Birth Defects Orig Art Ser 1993;29:83–102
Rutledge JC, Shourbaji AG, Hughes LA, Polifka JE, Cruz YP, Bishop JB, et al. Limb and lower-body duplications induced by retinoic acid in mice. Proc Natl Acad Sci USA 1994;91:5436–5440
Kroes HY, Takahashi M, Zijlstra RJ, Baert JA, Kooi KA, Hofstra RM, et al. Two cases of the caudal duplication anomaly including a discordant monozygotic twin. Am J Med Genet 2002;112:390–393
Carey JC, Greenbaum B, Hall BD. The OEIS complex (omphalocele, exstrophy, imperforate anus, spinal defects). Birth Defects Orig Artic Ser 1978;14(6B):253–263
Morimoto K, Takemoto O, Wakayama A. Tethered cord associated with anorectal malformation. Pediatr Neurosurg 2003;38:79–82
Hayden PW, Chapman WH, Stevenson JK. Exstrophy of the cloaca. Am J Dis Child 1973;125:879–883
Hurwitz R, Manzoni G, Ransley P, Stephens F. Cloacal exstrophy: a report of 34 cases. J Urol 1987;138:1060–1064
Wyburn GM. The development of the infra-umbilical portion of the abdominal wall, with remarks on the aetiology of ectopia vesicae. J Anat 1937;71:201–231
Vermeij-Keers C, Hartwig NG, van der Werff JF. Embryonic development of the ventral body wall and its congenital malformations. Semin Pediatr Surg 1996;5:82–89
Kluth D, Lambrecht W. Current concepts in the embryology of anorectal malformations. Semin Pediatr Surg 1997;6:180–186
Nievelstein RA, van der Werff JF, Verbeek FJ, Valk J, Vermeij-Keers C. Normal and abnormal embryonic development of the anorectum in human embryos. Teratology 1998;57:70–78
Paidas CN, Morreale RF, Holoski KM, Lund RE, Hutchins GM. Septation and differentiation of the embryonic human cloaca. J Pediatr Surg 1999;34:877–884
Bruch SW, Adzick NS, Goldstein RB, Harrison MR. Challenging the embryogenesis of cloacal exstrophy. J Pediatr Surg 1996;31:768–770
Lakshmanan Y, Bellin PB, Gilroy AM, Fung LCT. Antenatally diagnosed cloacal exstrophy variant with intravesical phallus in a twin pregnancy. Urology 2001;57:1178xii–1178xv
Langer JC, Brennan B, Lappalainen RE, Caco CC, Winthrop AL, Hollenberg RD, et al. Cloacal exstrophy: prenatal diagnosis before rupture of the cloacal membrane. J Pediatr Surg 1992;27:1352–1355
Mildenberger H, Kluth D, Dziuba M. Embryology of bladder exstrophy. J Pediatr Surg 1988;23:166–170
Koffler H, Aase J, Papile L-A, Coen R. Persistent cloaca with absent penis and anal atresia in one of identical twins. J Pediatr 1978;93:821–823
Wei X, Sulik KK. Pathogenesis of caudal dysgenesis/sirenomelia induced by Ochratoxin A in chick embryos. Teratology 1996;53:378–391
Manner J, Kluth D. A chicken model to study the embryology of cloacal exstrophy. J Pediatr Surg 2003;38:678–681
Manner J, Seidl W, Heinicke F, Hesse H. Teratogenic effects of suramin on the chick embryo. Anat Embryol 2003;206:229–237
Mo R, Kim JH, Zhang J, Chiang C, Hui C-C, Kim PCW. Anorectal malformations caused by defects in sonic hedgehog signaling. Am J Pathol 2001;159:765–774
Thompson DJ, Molello JA, Strebing RJ, Dyke IL. Teratogenicity of adriamycin and daunomycin in the rat and rabbit. Teratology 1978;17:151–158
Kramer R, Johnson M, Qureshi F, Jacques S, Yaron Y, Evans M. Concordance for cloacal dysgenesis. Fetal Diagn Ther 1997;12:279–282
Myrianthopoulos NC. Congenital malformations in twins: epidemiological survey. Birth Defects Orig Art Ser 1975;11:1–39
Schinzel A, Smith D, Miller J. Monozygotic twinning and structural defects. J Pediatr 1979;95:921–930
Spencer R. Conjoined Twins: Developmental Malformations and Clinical Implications. Baltimore: Johns Hopkins University Press, 2003
Cuschieri A, Group EW. Anorectal anomalies associated with or as part of other anomalies. Am J Med Genet 2002;110:122–130
Hall J. Twinning. Lancet 2003;362:735–743
Stoeckel W. Lehbuch der Geburtschilfe. Jena: Gustav Fischer, 1945
Boklage C. The frequency and survival probability of natural twin conceptions. In: Keith L, Papiernik E, Keith D, et al, eds. Multiple Pregnancy: Epidemiology, Gestation and Perinatal Outcome. New York: Parthenon, 1995;41–50
Hansen M, Kurinczuk JJ, Bower C, Webb S. The risk of major birth defects after intracytoplasmic sperm injection and in vitro fertilization. N Engl J Med 2002;346:725–730
Nakano H, Kubota S, Koyanagi T, Taki I. The prognosis of multiple pregnancy assessed by ultrasonic tomography. Acta Obstet Gynaecol Jpn 1981;33:839–843
Sampson A, de Crespigny LC. Vanishing twins: the frequency of spontaneous fetal reduction of a twin pregnancy. Ultrasound Obstet Gynecol 1992;2:107–109
Jackson J, Benirschke K. The recognition and significance of the vanishing twin. J Am Board Fam Pract 1989;2:58–63
Jauniaux E, Elkazen N, Leroy F, Wilkin P, Rodesch F, Hustin J. Clinical and morphologic aspects of the vanishing twin phenomenon. Obstet Gynecol 1988;72:577–581
Reddy KS, Petersen MB, Antonarakis SE, Blakemore KJ. The vanishing twin: an explanation for discordance between chorionic villus karyotype and fetal phenotype. Prenat Diagn 1991;11:679–684
Brodtkorb E, Myhr G, Gimse R. Is monochorionic twinning a risk factor for focal cortical dysgenesis? Acta Neurol Scand 2000;102:53–59
Kapur RP, Mahony BS, Nyberg DA, Resta RG, Shepard TH. Sirenomelia associated with a “vanishing twin”. Teratology 1991;43:103–108
Newton R, Casabonne D, Johnson A, Pharoah P. A case-control study of vanishing twin as a risk factor for cerebral palsy. Twin Res 2003;6:83–84
Poenaru D, Uroz-Tristan J, Leclerc S, Murphy S, St-Vil D, Youssef S, et al. Minimally conjoined omphalopagi: a consistent spectrum of anomalies. J Pediatr Surg 1994;29:1236–1238
Walton J, Gillis D, Giacomantonio J, Hayashi A, Lau H. Emergency separation of conjoined twins. J Pediatr Surg 1991;26:1337–1340
Spencer R. Minimally united ischiopagus twins: infraumbilical union with cloacal anomalies. J Pediatri Surg 1996;31:1538–1545
Smith D, Bartlett C, Harrah LM. Monozygotic twinning and the Duhamel anomalad (imperforate anus to sirenomelia): a nonrandom association between two aberrations in morphogenesis. Birth Defects Orig Art Ser 1976;12:53–63
Kapur RP, Jack RM, Siebert JR. Diamniotic placentation associated with omphalopagus conjoined twins: implications for a contemporary model of conjoined twinning. Am J Med Genet 1994;52:188–195
Benirschke K, Kaufmann P. Pathology of the Human Placenta. New York: Springer-Verlag, 2000
Gilbert SF. Developmental Biology, 5th ed. Sunderland, MA: Sinauer Associates, 1997
Tarkowski AK, Wroblewska J. Development of blastomeres of mouse eggs isolated at the 4- and 8-cell stage. J Embryol Exp Morphol 1967;18:155–180
Sheen TC, Chen SR, Au HK, Chien YY, Wu KY, Tzeng CR. Herniated blastomere following chemically assisted hatching may result in monozygotic twins. Fertil Steril 2001;75:443–444
Kaufman MH. The Atlas of Mouse Development. London: Academic Press, 1992
Popperl H, Schmidt C, Wilson V, Hume CR, Dodd J, Krumlauf R, et al. Misexpression of Cwnt8c in the mouse induces an ectopic embryonic axis and causes a truncation of the anterior neuroectoderm. Development 1997;124:2997–3005
Munro SS. Are monoovular avian twins always female. J Hered 1965;56:285–288
Spemann H. Embryonic Development and Induction. New York: Hafner Publishing, 1967
Stevens T, Siebert JR, Graham JJ, Beckwith JB. Parasitic conjoined twins, two cases, and their relation to limb morphogenesis. Teratology 1982;26:115–121
Spencer R. Parasitic conjoined twins: external, internal (fetuses in fetu and teratomas), and detached (acardiacs). Clin Anat 2001;14:428–444
Hsu YC, Gonda MA. Monozygotic twin formation in mouse embryos in vitro. Science 1980;209:605–606
Job CK, Sanchez RM, Kirchheimer WF, Hastings RC. Attempts to breed the nine-banded armadillo (Dasypus novemcinctus) in captivity—a preliminary report. Int J Lepr Other Mycobact Dis 1984;52:362–364
Newman HH, Patterson JT. The development of the nine-banded armadillo from the primitive streak stage to birth; with especial reference to the question of specific polyembryony. J Morphol 1910;21:359–424
Baldwin VJ. Pathology of Multiple Pregnancy. New York: Springer-Verlag, 1994
Machin GA. Conjoined twins: implications for blastogenesis. Birth Defects Orig Art Ser 1993;29:141–179
Machin GA, Sperber GH. Lessons from conjoined twins. Am J Med Genet 1987;28:89–97
Siebert JR, Machin GA, Sperber GH. Anatomic findings in dicephalic conjoined twins: implications for morphogenesis. Teratology 1989;40:305–310
Sperber GH, Machin GA. Microscopic study of midline determinants in janiceps twins. Birth Defects Orig Art Ser 1987;23:243–275
Spencer R. Conjoined twins: theoretical embryologic basis. Teratology 1992;45:591–602
Beckwith JB. Book review. Ped Dev Pathol 2003;6:361–364
Messmer BJ, Hornchen H, Kosters C. Surgical separation of conjoined (Siamese) xiphopagus twins. Surgery 1981;89:622–625
Martinez-Frias ML, Bermejo E, Rodriguez-Pinilla E, Frias JL. Exstrophy of the cloaca and exstrophy of the bladder: two different expressions of a primary developmental field defect. Am J Med Genet 2001;99:261–269
O’Rahilly R, Muller F. Human Embryology & Teratology. New York: Wiley-Liss, 1992
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We are grateful for the expert consultation afforded us in case 2 by Michael E. Mitchell, MD.
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Siebert, J.R., Rutledge, J.C. & Kapur, R.P. Association of Cloacal Anomalies, Caudal Duplication, and Twinning. Pediatr Dev Pathol 8, 339–354 (2005). https://doi.org/10.1007/s10024-005-1157-6
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DOI: https://doi.org/10.1007/s10024-005-1157-6