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Utility of immunohistochemical analysis of KAI1, epithelial-specific antigen, and epithelial-related antigen for distinction of chromophobe renal cell carcinoma, an eosinophilic variant from renal oncocytoma

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Abstract

Distinction of renal oncocytoma (RO) from chromophobe renal cell carcinoma (ChRCC) is important because their clinical behavior is different. As part of a search for the best available immunohistochemical markers to distinguish ChRCC from RO, we investigated the immunohistochemical profiles of these tumors. We selected 30 renal tumors consisting of ChRCC, typical variant (n = 14), ChRCC, eosinophilic variant (n = 6), and RO (n = 10). Their expression of cytokeratin (CK) 7, KAI1, epithelial-specific antigen (ESA), epithelial-related antigen (ERA), Claudin- 7, and Claudin-8 was studied using an autostainer. Immunoreactivity was assessed based on a combined score of the extent and intensity of staining. Compared to RO, a significantly higher percentage of the total ChRCCs stained positive for CK7 (85% vs. 10%, respectively), KAI1 (90% vs. 10%), ESA (95% vs. 10%), ERA (95% vs. 10%), and Claudin-7 (95% vs. 20%) (P < 0.001). Additionally, there was a significant difference between the percentage of ChRCC eosinophilic variant (ChRCC-E) and RO that stained positive for KAI1 (100% vs. 10%, respectively), ESA (83% vs. 10%), and ERA (83% vs. 10%) (P < 0.001). We recommend immunohistochemical analysis of KAI1, ESA, and ERA to distinguish ChRCC-E from RO.

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Authors and Affiliations

  1. Department of Pathology, Kansai Medical University, Hirakata Hospital, 2-3-1 Shinmachi, Hirakata, Osaka, 573-1191, Japan

    Chisato Ohe, Chika Miyasaka, Yorika Nakano, Noriko Sakaida & Yoshiko Uemura

  2. Department of Diagnostic Pathology, Kochi Red Cross Hospital, Kochi, Japan

    Naoto Kuroda

  3. Department of Pathology, Hyogo Prefectural Amagasaki Hospital, Hyogo, Japan

    Kosho Takasu

  4. Department of Pathology, Saiseikai Nakatsu Hospital, Osaka, Japan

    Chisato Ohe & Hideto Senzaki

  5. Department of Pathology, Kansai Medical University, Takii Hospital, Osaka, Japan

    Nobuaki Shikata

  6. Department of Cytopathology, Ayabe City Hospital, Kyoto, Japan

    Tadanori Yamaguchi

Authors
  1. Chisato Ohe
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  2. Naoto Kuroda
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  3. Kosho Takasu
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  4. Hideto Senzaki
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  5. Nobuaki Shikata
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  6. Tadanori Yamaguchi
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  7. Chika Miyasaka
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  8. Yorika Nakano
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  9. Noriko Sakaida
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  10. Yoshiko Uemura
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Correspondence to Yoshiko Uemura.

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Ohe, C., Kuroda, N., Takasu, K. et al. Utility of immunohistochemical analysis of KAI1, epithelial-specific antigen, and epithelial-related antigen for distinction of chromophobe renal cell carcinoma, an eosinophilic variant from renal oncocytoma. Med Mol Morphol 45, 98–104 (2012). https://doi.org/10.1007/s00795-011-0546-3

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  • DOI: https://doi.org/10.1007/s00795-011-0546-3

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