Skip to main content

Advertisement

SpringerLink
Log in

Identification of c-myc-dependent proteins in the medulloblastoma cell line D425Med

  • Original Article
  • Published:
Amino Acids Aims and scope Submit manuscript

Abstract

High c-myc levels are linked to poor prognosis in medulloblastoma (MB), and it was the aim of the current study to search for c-myc-dependent proteins in the MB cell line D425Med. For this purpose D425Med cells and cells with knocked-down c-myc (by siRNA) were analysed by a gel-based differential proteomics study using mass spectrometry. Heterogeneous nuclear ribonucleoproteins C1/C2, heterogeneous nuclear ribonucleoprotein A/B, stathmin, endoplasmic reticulum protein ERp29 precursor and guanidinoacetate N-methyltransferase were c-myc dependently expressed. Signalling, the protein machinery, metabolism and endoplasmic reticulum function may be affected and these results enable studying tumour tissue for these proteins as potential dignity markers or pharmacological targets.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

Abbreviations

2DE :

Two-dimensional gel electrophoresis

GAMT :

Guanidinoacetate N-methyltransferase

hnRNP :

Heterogeneous nuclear ribonucleoprotein

hTERT :

Human telomerase reverse transcriptase

MB:

Medulloblastoma

PNET :

Primitive neuroectodermal tumour

References

  • Ahn J, Murphy M, Kratowicz S, Wang A, Levine AJ, George DL (1999) Down-regulation of the stathmin/Op18 and FKBP25 genes following p53 induction. Oncogene 18:5954–5958

    Article  PubMed  CAS  Google Scholar 

  • Aldosari N, Bigner SH, Burger PC, Becker L, Kepner JL, Friedman HS, McLendon RE (2002) MYCC and MYCN oncogene amplification in medulloblastoma. A fluorescence in situ hybridization study on paraffin sections from the Children’s Oncology Group. Arch Pathol Lab Med 126:540–544

    PubMed  Google Scholar 

  • Alli E, Yang JM, Ford JM, Hait WN (2007a) Reversal of stathmin-mediated resistance to paclitaxel and vinblastine in human breast carcinoma cells. Mol Pharmacol 71:1233–1240

    Article  PubMed  CAS  Google Scholar 

  • Alli E, Yang JM, Hait WN (2007b) Silencing of stathmin induces tumor-suppressor function in breast cancer cell lines harboring mutant p53. Oncogene 26:1003–1012

    Article  PubMed  CAS  Google Scholar 

  • Azizi AA, Kang SU, Freilinger A, Gruber-Olipitz M, Chen WQ, Yang JW, Hengstschlager M, Slavc I, Lubec G (2008) Mitosis-dependent protein expression in neuroblastoma cell line N1E−115. J Proteome Res 7:3412–3422

    Article  PubMed  CAS  Google Scholar 

  • Bambang IF, Xu S, Zhou J, Salto-Tellez M, Sethi SK, Zhang D (2009) Overexpression of endoplasmic reticulum protein 29 regulates mesenchymal–epithelial transition and suppresses xenograft tumor growth of invasive breast cancer cells. Lab Invest 89:1229–1242

    Article  PubMed  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  PubMed  CAS  Google Scholar 

  • Braissant O, Bachmann C, Henry H (2007) Expression and function of AGAT, GAMT and CT1 in the mammalian brain. Subcell Biochem 46:67–81

    Article  PubMed  Google Scholar 

  • Brattsand G (2000) Correlation of oncoprotein 18/stathmin expression in human breast cancer with established prognostic factors. Br J Cancer 83:311–318

    Article  PubMed  CAS  Google Scholar 

  • Bullions LC, Levine AJ (1998) The role of beta-catenin in cell adhesion, signal transduction, and cancer. Curr Opin Oncol 10:81–87

    Article  PubMed  CAS  Google Scholar 

  • Chapman CA, Waber DP, Bernstein JH, Pomeroy SL, LaVally B, Sallan SE, Tarbell N (1995) Neurobehavioral and neurologic outcome in long-term survivors of posterior fossa brain tumors: role of age and perioperative factors. J Child Neurol 10:209–212

    Article  PubMed  CAS  Google Scholar 

  • Chen G, Wang H, Gharib TG, Huang CC, Thomas DG, Shedden KA, Kuick R, Taylor JM, Kardia SL, Misek DE, Giordano TJ, Iannettoni MD, Orringer MB, Hanash SM, Beer DG (2003) Overexpression of oncoprotein 18 correlates with poor differentiation in lung adenocarcinomas. Mol Cell Proteomics 2:107–116

    Article  PubMed  CAS  Google Scholar 

  • Chen WQ, Kang SU, Lubec G (2006) Protein profiling by the combination of two independent mass spectrometry techniques. Nat Protoc 1:1446–1452

    Article  PubMed  CAS  Google Scholar 

  • Cheretis C, Dietrich F, Chatzistamou I, Politi K, Angelidou E, Kiaris H, Mkrtchian S, Koutselini H (2006) Expression of ERp29, an endoplasmic reticulum secretion factor in basal-cell carcinoma. Am J Dermatopathol 28:410–412

    Article  PubMed  Google Scholar 

  • Choi H, Park CS, Kim BG, Cho JW, Park JB, Bae YS, Bae DS (2001) Creatine kinase B is a target molecule of reactive oxygen species in cervical cancer. Mol Cells 12:412–417

    PubMed  CAS  Google Scholar 

  • Christian KJ, Lang MA, Raffalli-Mathieu F (2008) Interaction of heterogeneous nuclear ribonucleoprotein C1/C2 with a novel cis-regulatory element within p53 mRNA as a response to cytostatic drug treatment. Mol Pharmacol 73:1558–1567

    Article  PubMed  CAS  Google Scholar 

  • Claassen GF, Hann SR (1999) Myc-mediated transformation: the repression connection. Oncogene 18:2925–2933

    Article  PubMed  CAS  Google Scholar 

  • Das P, Puri T, Suri V, Sharma MC, Sharma BS, Sarkar C (2009) Medulloblastomas: a correlative study of MIB-1 proliferation index along with expression of c-Myc, ERBB2, and anti-apoptotic proteins along with histological typing and clinical outcome. Childs Nerv Syst 25:825–835

    Article  PubMed  Google Scholar 

  • Dennis M, Spiegler BJ, Hetherington CR, Greenberg ML (1996) Neuropsychological sequelae of the treatment of children with medulloblastoma. J Neurooncol 29:91–101

    Article  PubMed  CAS  Google Scholar 

  • Eberhart CG, Tihan T, Burger PC (2000) Nuclear localization and mutation of beta-catenin in medulloblastomas. J Neuropathol Exp Neurol 59:333–337

    PubMed  CAS  Google Scholar 

  • Ellison DW, Onilude OE, Lindsey JC, Lusher ME, Weston CL, Taylor RE, Pearson AD, Clifford SC (2005) Beta-catenin status predicts a favorable outcome in childhood medulloblastoma: the United Kingdom Children’s Cancer Study Group Brain Tumour Committee. J Clin Oncol 23:7951–7957

    Article  PubMed  CAS  Google Scholar 

  • Fattet S, Haberler C, Legoix P, Varlet P, Lellouch-Tubiana A, Lair S, Manie E, Raquin MA, Bours D, Carpentier S, Barillot E, Grill J, Doz F, Puget S, Janoueix-Lerosey I, Delattre O (2009) Beta-catenin status in paediatric medulloblastomas: correlation of immunohistochemical expression with mutational status, genetic profiles, and clinical characteristics. J Pathol 218:86–94

    Article  PubMed  CAS  Google Scholar 

  • Feng Y, Tian ZM, Wan MX, Zheng ZB (2007) Protein profile of human hepatocarcinoma cell line SMMC-7721: identification and functional analysis. World J Gastroenterol 13:2608–2614

    PubMed  CAS  Google Scholar 

  • Giampietro C, Luzzati F, Gambarotta G, Giacobini P, Boda E, Fasolo A, Perroteau I (2005) Stathmin expression modulates migratory properties of GN-11 neurons in vitro. Endocrinology 146:1825–1834

    Article  PubMed  CAS  Google Scholar 

  • Gilbertson R, Wickramasinghe C, Hernan R, Balaji V, Hunt D, Jones-Wallace D, Crolla J, Perry R, Lunec J, Pearson A, Ellison D (2001) Clinical and molecular stratification of disease risk in medulloblastoma. Br J Cancer 85:705–712

    Article  PubMed  CAS  Google Scholar 

  • Goldwein JW, Radcliffe J, Johnson J, Moshang T, Packer RJ, Sutton LN, Rorke LB, D’Angio GJ (1996) Updated results of a pilot study of low dose craniospinal irradiation plus chemotherapy for children under five with cerebellar primitive neuroectodermal tumors (medulloblastoma). Int J Radiat Oncol Biol Phys 34:899–904

    Article  PubMed  CAS  Google Scholar 

  • Grotzer MA, Hogarty MD, Janss AJ, Liu X, Zhao H, Eggert A, Sutton LN, Rorke LB, Brodeur GM, Phillips PC (2001) MYC messenger RNA expression predicts survival outcome in childhood primitive neuroectodermal tumor/medulloblastoma. Clin Cancer Res 7:2425–2433

    PubMed  CAS  Google Scholar 

  • Grotzer MA, von Hoff K, von Bueren AO, Shalaby T, Hartmann W, Warmuth-Metz M, Emser A, Kortmann RD, Kuehl J, Pietsch T, Rutkowski S (2007) Which clinical and biological tumor markers proved predictive in the prospective multicenter trial HIT’91—implications for investigating childhood medulloblastoma. Klin Padiatr 219:312–317

    Article  PubMed  CAS  Google Scholar 

  • Gruber-Olipitz M, Strobel T, Kang SU, John JP, Grotzer MA, Slavc I, Lubec G (2009) Neurotrophin 3/TrkC-regulated proteins in the human medulloblastoma cell line DAOY. Electrophoresis 30:540–549

    Article  PubMed  CAS  Google Scholar 

  • Hait WN, Yang JM (2006) The individualization of cancer therapy: the unexpected role of p53. Trans Am Clin Climatol Assoc 117:85–101 (discussion 101)

    PubMed  Google Scholar 

  • Haley B, Paunesku T, Protic M, Woloschak GE (2009) Response of heterogeneous ribonuclear proteins (hnRNP) to ionising radiation and their involvement in DNA damage repair. Int J Radiat Biol 85:643–655

    Article  PubMed  CAS  Google Scholar 

  • Hamilton SR, Liu B, Parsons RE, Papadopoulos N, Jen J, Powell SM, Krush AJ, Berk T, Cohen Z, Tetu B et al (1995) The molecular basis of Turcot’s syndrome. N Engl J Med 332:839–847

    Article  PubMed  CAS  Google Scholar 

  • Harris MN, Ozpolat B, Abdi F, Gu S, Legler A, Mawuenyega KG, Tirado-Gomez M, Lopez-Berestein G, Chen X (2004) Comparative proteomic analysis of all-trans-retinoic acid treatment reveals systematic posttranscriptional control mechanisms in acute promyelocytic leukemia. Blood 104:1314–1323

    Article  PubMed  CAS  Google Scholar 

  • He TC, Sparks AB, Rago C, Hermeking H, Zawel L, da Costa LT, Morin PJ, Vogelstein B, Kinzler KW (1998) Identification of c-MYC as a target of the APC pathway. Science 281:1509–1512

    Article  PubMed  CAS  Google Scholar 

  • Henriksson M, Luscher B (1996) Proteins of the Myc network: essential regulators of cell growth and differentiation. Adv Cancer Res 68:109–182

    Article  PubMed  CAS  Google Scholar 

  • Heo S, Lubec G (2010) Generation and characterization of a specific polyclonal antibody against the mouse serotonin receptor 1A: a state-of-the-art recommendation on how to characterize antibody specificity. Electrophoresis 31:3789–3796

    Article  PubMed  CAS  Google Scholar 

  • Herms J, Neidt I, Luscher B, Sommer A, Schurmann P, Schroder T, Bergmann M, Wilken B, Probst-Cousin S, Hernaiz-Driever P, Behnke J, Hanefeld F, Pietsch T, Kretzschmar HA (2000) C-MYC expression in medulloblastoma and its prognostic value. Int J Cancer 89:395–402

    Article  PubMed  CAS  Google Scholar 

  • Hossain MN, Fuji M, Miki K, Endoh M, Ayusawa D (2007) Downregulation of hnRNP C1/C2 by siRNA sensitizes HeLa cells to various stresses. Mol Cell Biochem 296:151–157

    Article  PubMed  CAS  Google Scholar 

  • Ide T, Brown-Endres L, Chu K, Ongusaha PP, Ohtsuka T, El-Deiry WS, Aaronson SA, Lee SW (2009) GAMT, a p53-inducible modulator of apoptosis, is critical for the adaptive response to nutrient stress. Mol Cell 36:379–392

    Article  PubMed  CAS  Google Scholar 

  • Jemal A, Siegel R, Ward E, Hao Y, Xu J, Murray T, Thun MJ (2008) Cancer statistics, 2008. CA Cancer J Clin 58:71–96

    Article  PubMed  Google Scholar 

  • Jin K, Mao XO, Cottrell B, Schilling B, Xie L, Row RH, Sun Y, Peel A, Childs J, Gendeh G, Gibson BW, Greenberg DA (2004) Proteomic and immunochemical characterization of a role for stathmin in adult neurogenesis. Faseb J 18:287–299

    Article  PubMed  CAS  Google Scholar 

  • Kim JH, Paek KY, Choi K, Kim TD, Hahm B, Kim KT, Jang SK (2003) Heterogeneous nuclear ribonucleoprotein C modulates translation of c-myc mRNA in a cell cycle phase-dependent manner. Mol Cell Biol 23:708–720

    Article  PubMed  CAS  Google Scholar 

  • Kokkinakis DM, Liu X, Chada S, Ahmed MM, Shareef MM, Singha UK, Yang S, Luo J (2004) Modulation of gene expression in human central nervous system tumors under methionine deprivation-induced stress. Cancer Res 64:7513–7525

    Article  PubMed  CAS  Google Scholar 

  • Korshunov A, Benner A, Remke M, Lichter P, von Deimling A, Pfister S (2008) Accumulation of genomic aberrations during clinical progression of medulloblastoma. Acta Neuropathol 116:383–390

    Article  PubMed  CAS  Google Scholar 

  • Kouzu Y, Uzawa K, Koike H, Saito K, Nakashima D, Higo M, Endo Y, Kasamatsu A, Shiiba M, Bukawa H, Yokoe H, Tanzawa H (2006) Overexpression of stathmin in oral squamous-cell carcinoma: correlation with tumour progression and poor prognosis. Br J Cancer 94:717–723

    PubMed  CAS  Google Scholar 

  • Kuo MF, Wang HS, Kuo QT, Shun CT, Hsu HC, Yang SH, Yuan RH (2009) High expression of stathmin protein predicts a fulminant course in medulloblastoma. J Neurosurg Pediatr 4:74–80

    Article  PubMed  Google Scholar 

  • Liu J, Levens D (2006) Making myc. Curr Top Microbiol Immunol 302:1–32

    Article  PubMed  CAS  Google Scholar 

  • MacDonald TJ, Rood BR, Santi MR, Vezina G, Bingaman K, Cogen PH, Packer RJ (2003) Advances in the diagnosis, molecular genetics, and treatment of pediatric embryonal CNS tumors. Oncologist 8:174–186

    Article  PubMed  Google Scholar 

  • MacLeod JC, Sayer RJ, Lucocq JM, Hubbard MJ (2004) ERp29, a general endoplasmic reticulum marker, is highly expressed throughout the brain. J Comp Neurol 477:29–42

    Article  PubMed  CAS  Google Scholar 

  • Meffert G, Gellerich FN, Margreiter R, Wyss M (2005) Elevated creatine kinase activity in primary hepatocellular carcinoma. BMC Gastroenterol 5:9

    Article  PubMed  Google Scholar 

  • Melhem RF, Zhu XX, Hailat N, Strahler JR, Hanash SM (1991) Characterization of the gene for a proliferation-related phosphoprotein (oncoprotein 18) expressed in high amounts in acute leukemia. J Biol Chem 266:17747–17753

    PubMed  CAS  Google Scholar 

  • Mistry SJ, Bank A, Atweh GF (2007) Synergistic antiangiogenic effects of stathmin inhibition and taxol exposure. Mol Cancer Res 5:773–782

    Article  PubMed  CAS  Google Scholar 

  • Mkrtchian S, Baryshev M, Sargsyan E, Chatzistamou I, Volakaki AA, Chaviaras N, Pafiti A, Triantafyllou A, Kiaris H (2008) ERp29, an endoplasmic reticulum secretion factor is involved in the growth of breast tumor xenografts. Mol Carcinog 47:886–892

    Article  PubMed  CAS  Google Scholar 

  • Mori T, Nagase H, Horii A, Miyoshi Y, Shimano T, Nakatsuru S, Aoki T, Arakawa H, Yanagisawa A, Ushio Y et al (1994) Germ-line and somatic mutations of the APC gene in patients with Turcot syndrome and analysis of APC mutations in brain tumors. Genes Chromosomes Cancer 9:168–172

    Article  PubMed  CAS  Google Scholar 

  • Neben K, Korshunov A, Benner A, Wrobel G, Hahn M, Kokocinski F, Golanov A, Joos S, Lichter P (2004) Microarray-based screening for molecular markers in medulloblastoma revealed STK15 as independent predictor for survival. Cancer Res 64:3103–3111

    Article  PubMed  CAS  Google Scholar 

  • Nesbit CE, Tersak JM, Prochownik EV (1999) MYC oncogenes and human neoplastic disease. Oncogene 18:3004–3016

    Article  PubMed  CAS  Google Scholar 

  • Ngo TT, Peng T, Liang XJ, Akeju O, Pastorino S, Zhang W, Kotliarov Y, Zenklusen JC, Fine HA, Maric D, Wen PY, De Girolami U, Black PM, Wu WW, Shen RF, Jeffries NO, Kang DW, Park JK (2007) The 1p-encoded protein stathmin and resistance of malignant gliomas to nitrosoureas. J Natl Cancer Inst 99:639–652

    Article  PubMed  CAS  Google Scholar 

  • Obaya AJ, Mateyak MK, Sedivy JM (1999) Mysterious liaisons: the relationship between c-Myc and the cell cycle. Oncogene 18:2934–2941

    Article  PubMed  CAS  Google Scholar 

  • Packer RJ (2008) Childhood brain tumors: accomplishments and ongoing challenges. J Child Neurol 23:1122–1127

    Article  PubMed  Google Scholar 

  • Pfister S, Remke M, Benner A, Mendrzyk F, Toedt G, Felsberg J, Wittmann A, Devens F, Gerber NU, Joos S, Kulozik A, Reifenberger G, Rutkowski S, Wiestler OD, Radlwimmer B, Scheurlen W, Lichter P, Korshunov A (2009) Outcome prediction in pediatric medulloblastoma based on DNA copy-number aberrations of chromosomes 6q and 17q and the MYC and MYCN loci. J Clin Oncol 27:1627–1636

    Article  PubMed  Google Scholar 

  • Prendergast GC (1999) Mechanisms of apoptosis by c-Myc. Oncogene 18:2967–2987

    Article  PubMed  CAS  Google Scholar 

  • Radcliffe J, Packer RJ, Atkins TE, Bunin GR, Schut L, Goldwein JW, Sutton LN (1992) Three- and four-year cognitive outcome in children with noncortical brain tumors treated with whole-brain radiotherapy. Ann Neurol 32:551–554

    Article  PubMed  CAS  Google Scholar 

  • Ray A, Ho M, Ma J, Parkes RK, Mainprize TG, Ueda S, McLaughlin J, Bouffet E, Rutka JT, Hawkins CE (2004) A clinicobiological model predicting survival in medulloblastoma. Clin Cancer Res 10:7613–7620

    Article  PubMed  CAS  Google Scholar 

  • Rogers HA, Miller S, Lowe J, Brundler MA, Coyle B, Grundy RG (2009) An investigation of WNT pathway activation and association with survival in central nervous system primitive neuroectodermal tumours (CNS PNET). Br J Cancer 100:1292–1302

    Article  PubMed  CAS  Google Scholar 

  • Rubin CI, Atweh GF (2004) The role of stathmin in the regulation of the cell cycle. J Cell Biochem 93:242–250

    Article  PubMed  CAS  Google Scholar 

  • Rutkowski S, von Bueren A, von Hoff K, Hartmann W, Shalaby T, Deinlein F, Warmuth-Metz M, Soerensen N, Emser A, Bode U, Mittler U, Urban C, Benesch M, Kortmann RD, Schlegel PG, Kuehl J, Pietsch T, Grotzer M (2007) Prognostic relevance of clinical and biological risk factors in childhood medulloblastoma: results of patients treated in the prospective multicenter trial HIT’91. Clin Cancer Res 13:2651–2657

    Article  PubMed  CAS  Google Scholar 

  • Rutkowski S, Gerber NU, von Hoff K, Gnekow A, Bode U, Graf N, Berthold F, Henze G, Wolff JE, Warmuth-Metz M, Soerensen N, Emser A, Ottensmeier H, Deinlein F, Schlegel PG, Kortmann RD, Pietsch T, Kuehl J (2009) Treatment of early childhood medulloblastoma by postoperative chemotherapy and deferred radiotherapy. Neuro Oncol 11:201–210

    Article  PubMed  CAS  Google Scholar 

  • Sargsyan E, Baryshev M, Szekely L, Sharipo A, Mkrtchian S (2002) Identification of ERp29, an endoplasmic reticulum lumenal protein, as a new member of the thyroglobulin folding complex. J Biol Chem 277:17009–17015

    Article  PubMed  CAS  Google Scholar 

  • Scheurlen WG, Schwabe GC, Joos S, Mollenhauer J, Sorensen N, Kuhl J (1998) Molecular analysis of childhood primitive neuroectodermal tumors defines markers associated with poor outcome. J Clin Oncol 16:2478–2485

    PubMed  CAS  Google Scholar 

  • Schmidt EV (1999) The role of c-myc in cellular growth control. Oncogene 18:2988–2996

    Article  PubMed  CAS  Google Scholar 

  • Silber JH, Radcliffe J, Peckham V, Perilongo G, Kishnani P, Fridman M, Goldwein JW, Meadows AT (1992) Whole-brain irradiation and decline in intelligence: the influence of dose and age on IQ score. J Clin Oncol 10:1390–1396

    PubMed  CAS  Google Scholar 

  • Siu IM, Lal A, Blankenship JR, Aldosari N, Riggins GJ (2003) c-Myc promoter activation in medulloblastoma. Cancer Res 63:4773–4776

    PubMed  CAS  Google Scholar 

  • Spahn A, Blondeau N, Heurteaux C, Dehghani F, Rami A (2008) Concomitant transitory up-regulation of X-linked inhibitor of apoptosis protein (XIAP) and the heterogeneous nuclear ribonucleoprotein C1–C2 in surviving cells during neuronal apoptosis. Neurochem Res 33:1859–1868

    Article  PubMed  CAS  Google Scholar 

  • Stearns D, Chaudhry A, Abel TW, Burger PC, Dang CV, Eberhart CG (2006) c-myc overexpression causes anaplasia in medulloblastoma. Cancer Res 66:673–681

    Article  PubMed  CAS  Google Scholar 

  • Stockler S, Schutz PW, Salomons GS (2007) Cerebral creatine deficiency syndromes: clinical aspects, treatment and pathophysiology. Subcell Biochem 46:149–166

    Article  PubMed  Google Scholar 

  • Sun W, Xing B, Sun Y, Du X, Lu M, Hao C, Lu Z, Mi W, Wu S, Wei H, Gao X, Zhu Y, Jiang Y, Qian X, He F (2007) Proteome analysis of hepatocellular carcinoma by two-dimensional difference gel electrophoresis: novel protein markers in hepatocellular carcinoma tissues. Mol Cell Proteomics 6:1798–1808

    Article  PubMed  CAS  Google Scholar 

  • Takahashi M, Yang XJ, Lavery TT, Furge KA, Williams BO, Tretiakova M, Montag A, Vogelzang NJ, Re GG, Garvin AJ, Soderhall S, Kagawa S, Hazel-Martin D, Nordenskjold A, Teh BT (2002) Gene expression profiling of favorable histology Wilms tumors and its correlation with clinical features. Cancer Res 62:6598–6605

    PubMed  CAS  Google Scholar 

  • von Bueren AO, Shalaby T, Rajtarova J, Stearns D, Eberhart CG, Helson L, Arcaro A, Grotzer MA (2007) Anti-proliferative activity of the quassinoid NBT-272 in childhood medulloblastoma cells. BMC Cancer 7:19

    Article  Google Scholar 

  • von Bueren AO, Shalaby T, Oehler-Janne C, Arnold L, Stearns D, Eberhart CG, Arcaro A, Pruschy M, Grotzer MA (2009) RNA interference-mediated c-MYC inhibition prevents cell growth and decreases sensitivity to radio- and chemotherapy in childhood medulloblastoma cells. BMC Cancer 9:10

    Article  Google Scholar 

  • von Hoff K, Hinkes B, Gerber NU, Deinlein F, Mittler U, Urban C, Benesch M, Warmuth-Metz M, Soerensen N, Zwiener I, Goette H, Schlegel PG, Pietsch T, Kortmann RD, Kuehl J, Rutkowski S (2009) Long-term outcome and clinical prognostic factors in children with medulloblastoma treated in the prospective randomised multicentre trial HIT’91. Eur J Cancer 45:1209–1217

    Article  Google Scholar 

  • von Hoff K, Hartmann W, von Bueren AO, Gerber NU, Grotzer MA, Pietsch T, Rutkowski S (2010) Large cell/anaplastic medulloblastoma: outcome according to myc status, histopathological, and clinical risk factors. Pediatr Blood Cancer 54:369–376

    Article  Google Scholar 

  • Wei SH, Lin F, Wang X, Gao P, Zhang HZ (2008) Prognostic significance of stathmin expression in correlation with metastasis and clinicopathological characteristics in human ovarian carcinoma. Acta Histochem 110:59–65

    Article  PubMed  CAS  Google Scholar 

  • Yuan RH, Jeng YM, Chen HL, Lai PL, Pan HW, Hsieh FJ, Lin CY, Lee PH, Hsu HC (2006) Stathmin overexpression cooperates with p53 mutation and osteopontin overexpression, and is associated with tumour progression, early recurrence, and poor prognosis in hepatocellular carcinoma. J Pathol 209:549–558

    Article  PubMed  CAS  Google Scholar 

  • Zeltzer PM, Boyett JM, Finlay JL, Albright AL, Rorke LB, Milstein JM, Allen JC, Stevens KR, Stanley P, Li H, Wisoff JH, Geyer JR, McGuire-Cullen P, Stehbens JA, Shurin SB, Packer RJ (1999) Metastasis stage, adjuvant treatment, and residual tumor are prognostic factors for medulloblastoma in children: conclusions from the Children’s Cancer Group 921 randomized phase III study. J Clin Oncol 17:832–845

    PubMed  CAS  Google Scholar 

  • Zurawel RH, Chiappa SA, Allen C, Raffel C (1998) Sporadic medulloblastomas contain oncogenic beta-catenin mutations. Cancer Res 58:896–899

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was mainly financed by a grant of the Jubiläumsfonds der Österreichische Nationalbank (OeNB) (Jubiläumsfondsprojekt Nr. 12195). The project was in part supported by the Forschungsgesellschaft für cerebrale Tumore.

Conflict of interest

The authors state that there is no conflict of interests.

Author information

Authors and Affiliations

  1. Department of Pediatrics, Medical University of Vienna, Währinger Gürtel 18, 1090, Vienna, Austria

    Amedeo A. Azizi, Lin Li, Wei-Qiang Chen, Irene Slavc & Gert Lubec

  2. Institute of Neurology, Medical University of Vienna, Währinger Gürtel 18, 1090, Vienna, Austria

    Thomas Ströbel

Authors
  1. Amedeo A. Azizi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lin Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Thomas Ströbel
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wei-Qiang Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Irene Slavc
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Gert Lubec
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Thomas Ströbel or Gert Lubec.

Additional information

A. A. Azizi and L. Li contributed equally to this work.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 160 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Azizi, A.A., Li, L., Ströbel, T. et al. Identification of c-myc-dependent proteins in the medulloblastoma cell line D425Med. Amino Acids 42, 2149–2163 (2012). https://doi.org/10.1007/s00726-011-0953-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00726-011-0953-8

Keywords

Search

Navigation