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Mild hypothermia protects against oxygen glucose deprivation (OGD)-induced cell death in brain slices from adult mice

  • Translational Neurosciences - Short communication
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Abstract

Most of neuroprotective strategies in stroke have failed to move from bench to bedside. One explanation might be the use of excessive uniform and smooth experimental models. Therefore, we have employed a more stringent in vitro model based on cultured brain slices from adult mice submitted to OGD. Using this acute model, we have confirmed that mild hypothermia protects against OGD-induced cell death when cooling the tissue during and after OGD, but not when hypothermia is induced only during reoxygenation.

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Fig. 1
Fig. 2

Abbreviations

OGD:

Oxygen glucose deprivation

PBS:

Phosphate buffered saline

LDH:

Lactate dehydrogenase

MTT:

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

PI:

Propidium iodide

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Acknowledgments

M.H-G. and A.R. are supported by the Miguel Servet programme (CP09/00265 and CP12/03259, respectively) and M.C-M and C.M. by Fondo de Investigación Sanitaria grants (FI 10/00508 and FI12/00089, respectively) from Instituto de Salud Carlos III. The research leading to these results is funded by the European Community’s Seventh Framework Programme (FP7/2007–2013) under the EuroHYP-1 grant agreement no 278709. The Neurovascular Research Lab takes part into the Spanish stroke research network INVICTUS (RD12/0014/0005). We thank Professor Lopez MG (Instituto Teófilo Hernando, Universidad Autónoma de Madrid) for her kind advice in the set-up of the model.

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The authors declare no conflict of interest.

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Authors and Affiliations

  1. Neurovascular Research Laboratory, Institut de Recerca, Vall d’Hebron Hospital, Universitat Autònoma de Barcelona, Pg Vall d’Hebron 119-129, 08035, Barcelona, Spain

    Mar Hernández-Guillamon, Laura Ortega, Cristina Merino-Zamorano, Mireia Campos-Martorell, Anna Rosell & Joan Montaner

  2. Neurovascular Unit, Neurology Department, Vall d’Hebron Hospital, Universitat Autònoma de Barcelona, Barcelona, Spain

    Joan Montaner

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  1. Mar Hernández-Guillamon
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  2. Laura Ortega
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  3. Cristina Merino-Zamorano
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  4. Mireia Campos-Martorell
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  5. Anna Rosell
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  6. Joan Montaner
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Correspondence to Joan Montaner.

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Hernández-Guillamon, M., Ortega, L., Merino-Zamorano, C. et al. Mild hypothermia protects against oxygen glucose deprivation (OGD)-induced cell death in brain slices from adult mice. J Neural Transm 121, 113–117 (2014). https://doi.org/10.1007/s00702-013-1090-1

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  • DOI: https://doi.org/10.1007/s00702-013-1090-1

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