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Human mesenchymal stem cells and biomaterials interaction: a promising synergy to improve spine fusion

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Abstract

Purpose

Spine fusion is the gold standard treatment in degenerative and traumatic spine diseases. The bone regenerative medicine needs (i) in vitro functionally active osteoblasts, and/or (ii) the in vivo induction of the tissue. The bone tissue engineering seems to be a very promising approach for the effectiveness of orthopedic surgical procedures, clinical applications are often hampered by the limited availability of bone allograft or substitutes. New biomaterials have been recently developed for the orthopedic applications. The main characteristics of these scaffolds are the ability to induce the bone tissue formation by generating an appropriate environment for (i) the cell growth and (ii) recruiting precursor bone cells for the proliferation and differentiation. A new prototype of biomaterials known as “bioceramics” may own these features. Bioceramics are bone substitutes mainly composed of calcium and phosphate complex salt derivatives.

Methods

In this study, the characteristics bioceramics bone substitutes have been tested with human mesenchymal stem cells obtained from the bone marrow of adult orthopedic patients.

Results

These cellular models can be employed to characterize in vitro the behavior of different biomaterials, which are used as bone void fillers or three-dimensional scaffolds.

Conclusions

Human mesenchymal stem cells in combination with biomaterials seem to be good alternative to the autologous or allogenic bone fusion in spine surgery. The cellular model used in our study is a useful tool for investigating cytocompatibility and biological features of HA-derived scaffolds.

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References

  1. Minamide A, Kawakami M, Hashizume H, Sakata R, Tamaki T (2001) Evaluation of carriers of bone morphogenetic protein for spinal fusion. Spine 26:933–939

    Article  PubMed  CAS  Google Scholar 

  2. Liao SS, Guan K, Cui FZ, Shi SS, Sun TS (2003) Lumbar spinal fusion with a mineralized collagen matrix and rhBMP-2 in a rabbit model. Spine 28:1954–1960

    Article  PubMed  CAS  Google Scholar 

  3. Cui Q, Ming Xiao Z, Balian G, Wang GJ (2001) Comparison of lumbar spine fusion using mixed and cloned marrow cells. Spine 26:2305–2310

    Article  PubMed  CAS  Google Scholar 

  4. Peterson B, Iglesias R, Zhang J, Wang JC, Lieberman JR (2005) Genetically modified human derived bone marrow cells for posterolateral lumbar spine fusion in athymic rats: beyond conventional autologous bone grafting. Spine 30:283–289

    Article  PubMed  Google Scholar 

  5. De Long WG, Einhorn TA Jr, Koval K, McKee M, Smith W, Sanders R, Watson T (2007) Bone grafts and bone graft substitutes in orthopaedic trauma surgery. A critical analysis. J Bone Jt Surg Am 89:649–658

    Article  Google Scholar 

  6. Jenis L (2006) Synthetic bone grafts in orthopaedic surgery. US Musculoskeletal Rev 2:48–49

    Google Scholar 

  7. Gazdag AR, Lane JM, Glaser D, Forster RA (1995) Alternatives to autogenous bone graft: efficacy and indications. J Am Acad Orthop Surg 3:1–8

    PubMed  Google Scholar 

  8. Aaboe M, Pinholt EM, Hjortinghansen E (1995) Healing of experimentally created defects—a review. Br J Oral Maxillofac Surg 33:312–318

    Article  PubMed  CAS  Google Scholar 

  9. Summers BN, Eisenstein SM (1989) Donor site pain from the ilium. A complication of lumbar spine fusion. J Bone Jt Surg Br 71:677–680

    CAS  Google Scholar 

  10. Devin JE, Attawia MA, Laurencin CT (1996) Three-dimensional degradable porous polymer-ceramic matrices for use in bone repair. J Biomater Sci Polym Ed 7:661–669

    Article  PubMed  CAS  Google Scholar 

  11. Solchaga LA, Hee CK, Aguiar DJ, Ratliff J, Turner AS, Seim 3rd HB, Hollinger JO, Snel LB, Lynch SE (2011) Augment® bone graft products compare favorably to autologous bone graft in an ovine model of lumbar interbody spine fusion. Spine (Phila Pa 1976). 2011 Oct 21 [Epub ahead of print]

  12. Kang J, An H, Hilibrand A, Yoon ST, Kavanagh E, Boden S (2011) Grafton® & local bone has comparable outcomes to iliac crest bone in instrumented single level lumbar fusions. Spine (Phila Pa 1976). 2011 Nov 8 [Epub ahead of print]

  13. Kerr EJ 3rd, Jawahar A, Wooten T, Kay S, Cavanaugh DA, Nunley PD (2011) The use of osteo-conductive stem-cells allograft in lumbar interbody fusion procedures: an alternative to recombinant human bone morphogenetic protein. J Surg Orthop Adv 20(3):193–197

    PubMed  Google Scholar 

  14. Huang JW, Lin SS, Chen LH, Liu SJ, Niu CC, Yuan LJ, Wu CC, Chen WJ (2011) The use of fluorescence-labeled mesenchymal stem cells in poly(lactide-co-glycolide)/hydroxyapatite/collagen hybrid graft as a bone substitute for posterolateral spinal fusion. J Trauma 70(6):1495–1502

    Article  PubMed  CAS  Google Scholar 

  15. Yamada T, Yoshii T, Sotome S, Yuasa M, Kato T, Arai Y, Kawabata S, Tomizawa S, Sakaki K, Hirai T, Shinomiya K, Okawa A (2011) hybrid grafting using bone marrow aspirate combined with porous β-tricalcium phosphate and trephine bone for lumbar posterolateral spinal fusion: a prospective, comparative study—versus local bone grafting. Spine (Phila Pa 1976). 2011 Jun 13 [Epub ahead of print]

  16. Buhring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W (2007) Novel markers for the prospective isolation of human MSC. Ann NY Acad Sci 1106:262–271

    Article  PubMed  Google Scholar 

  17. DiGirolamo GJ, Gilchrist ID, Brown V, Findlay JM (1999) The ability to shift covert attention is not dependent on the experience of moving the eyes: a case study. J Cogn Neurosci:70–71

  18. Weinstein JN, Lurie JD, Tosteson TD, Hanscom B, Tosteson AN, Blood EA, Birkmeyer NJ, Hilibrand AS, Herkowitz H, Cammisa FP, Albert TJ, Emery SE, Lenke LG, Abdu WA, Longley M, Errico TJ, Hu SS (2007) Surgical versus nonsurgical treatment for lumbar degenerative spondylolisthesis. N Engl J Med 356:2257–2270

    Article  PubMed  CAS  Google Scholar 

  19. Baksh D, Song L, Tuan RS (2004) Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy. J Cell Mol Med 8:301–316

    Article  PubMed  CAS  Google Scholar 

  20. Chou YF, Huang W, Dunn JC, Miller TA, Wu BM (2005) The effect of biomimetic apatite structure on osteoblast viability, proliferation, and gene expression. Biomaterials 26:285–295

    Article  PubMed  CAS  Google Scholar 

  21. Hamilton DW, Brunette DM (2007) The effect of substratum topography on osteoblast adhesion mediated signal transduction and phosphorylation. Biomaterials 28:1806–1819

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We thank Dr. Carlo Piovani for his technical assistance in performing the bone marrow aspirates during surgery at the Orthopedic Institute “Rizzoli”, Bologna, Italy. This study was supported, in part, by grants from Fondazione Cassa di Risparmio di Bologna, Bologna, Fondazione Cassa di Risparmio di Cento, Cento, Regione Emilia-Romagna and University of Ferrara, Ferrara. Italy.

Conflict of interest

None.

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Authors and Affiliations

  1. Department of Morphology and Embryology, Section of Cell Biology and Molecular Genetics, University of Ferrara, Fossato di Mortara 64/B, 44121, Ferrara, Italy

    E. Mazzoni, M. Tognon & M. Manfrini

  2. Oncologic and Degenerative Spine Surgery Department, Istituto Ortopedico Rizzoli, G. C.Pupilli, 1, 40136, Bologna, Italy

    G. Barbanti Brodano & C. Griffoni

Authors
  1. G. Barbanti Brodano
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  2. E. Mazzoni
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  3. M. Tognon
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  4. C. Griffoni
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  5. M. Manfrini
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Corresponding author

Correspondence to M. Manfrini.

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Barbanti Brodano, G., Mazzoni, E., Tognon, M. et al. Human mesenchymal stem cells and biomaterials interaction: a promising synergy to improve spine fusion. Eur Spine J 21 (Suppl 1), 3–9 (2012). https://doi.org/10.1007/s00586-012-2233-z

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  • DOI: https://doi.org/10.1007/s00586-012-2233-z

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