Abstract
Hypoxia induces a series of adaptive physiological responses including gene inductions. Hypoxia-inducible factor 1 (HIF-1) is a master transcription factor that regulates hypoxia-induced gene expression to maintain homeostasis in the living body. Opioids are potent analgesic agents that are widely used in clinical practice. Therefore, we investigated the effect of opioids on HIF-1 activity. SH-SY5Y human neuronal cells, which express opioid receptors intrinsically, were cultured under 1% or 20% O2 conditions with or without treatment by DAGO, DPDPE, or U-50488, which are the selective agonists of µ-, δ-, and κ-opioid receptors, respectively. Expression of subunits of HIF-1, HIF-1α, and HIF-1β were examined by Western blot using specific antibodies. Expression of the HIF-1-dependent gene were investigated by reporter assay. None of the selective agonists of opioid receptors tested affected HIF-1 activation by hypoxia. Therefore, it is suggested that opioid receptor-mediated signals do not affect HIF-1-dependent cellular hypoxia-induced gene responses.
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References
K Hirota (2002) ArticleTitleHypoxia-inducible factor 1, a master transcription factor of cellular hypoxic gene expression J Anesth 16 150–159
GL Wang BH Jiang EA Rue GL Semenza (1995) ArticleTitleHypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension Proc Natl Acad Sci USA 92 5510–5514
GL Semenza (2001) ArticleTitleHIF-1, O2, and the 3 PHDs: how animal cells signal hypoxia to the nucleus Cell 107 1–3
R Fukuda K Hirota F Fan YD Jung LM Ellis GL Semenza (2002) ArticleTitleInsulin-like growth factor 1 induces hypoxia-inducible factor 1-mediated vascular endothelial growth factor expression, which is dependent on MAP kinase and phosphatidylinositol 3-kinase signaling in colon cancer cells J Biol Chem 277 38205–38211
R Fukuda B Kelly GL Semenza (2003) ArticleTitleVascular endothelial growth factor gene expression in colon cancer cells exposed to prostaglandin E2 is mediated by hypoxia-inducible factor 1 Cancer Res 63 2330–2334
K Kasuno S Takabuchi K Fukuda S Kizaka-Kondoh J Yodoi T Adachi GL Semenza K Hirota (2004) ArticleTitleNitric oxide induces hypoxia-inducible factor 1 activation that is dependent on MAPK and phosphatidylinositol 3-kinase signaling J Biol Chem 279 2550–2558
K Hirota R Fukuda S Takabuchi S Kizaka-Kondoh T Adachi K Fukuda GL Semenza (2004) ArticleTitleInduction of hypoxia-inducible factor 1 activity by muscarinic acetylcholine receptor signaling J Biol Chem 279 41521–41528
T Itoh T Namba K Fukuda GL Semenza K Hirota (2001) ArticleTitleReversible inhibition of hypoxia-inducible factor 1 activation by exposure of hypoxic cells to the volatile anesthetic halothane FEBS Lett 509 225–229
S Takabuchi K Hirota K Nishi S Oda T Oda T Shingu A Takabayashi T Adachi GL Semenza K Fukuda (2004) ArticleTitleThe intravenous anesthetic propofol inhibits hypoxia-inducible factor 1 activity in an oxygen tension dependent manner. FEBS Lett 577 434–438
K Nishi K Hirota S Takabuchi S Oda K Fukuda T Adachi T Shingu (2005) ArticleTitleThe effect of local anesthetics on the cellular hypoxia-induced gene responses mediated by hypoxia-inducible factor 1 J Anesth 19 54–59
K Hirota GL Semenza (2001) ArticleTitleRac1 activity is required for the activation of hypoxia-inducible factor 1 J Biol Chem 276 21166–21172
BA Jordan LA Devi (1999) ArticleTitleG-protein-coupled receptor heterodimerization modulates receptor function Nature 399 697–700
S Pepe OW van den Brink EG Lakatta RP Xiao (2004) ArticleTitleCross-talk of opioid peptide receptor and beta-adrenergic receptor signalling in the heart Cardiovasc Res 63 414–422
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Takabuchi, S., Hirota, K., Oda, S. et al. Opioid receptor stimulation does not affect cellular hypoxia-induced gene responses mediated by hypoxia-inducible factor 1 in cultured cell lines. J Anesth 19, 263–265 (2005). https://doi.org/10.1007/s00540-005-0327-z
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DOI: https://doi.org/10.1007/s00540-005-0327-z