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Microsomal prostaglandin E synthase protein levels correlate with prognosis in colorectal cancer patients

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Abstract

The aim of this study is to investigate the expression of three prostaglandin E synthase (PGES) isomers in colorectal cancer (CRC) tissue and to evaluate their relationship to clinicopathological factors and patient prognosis. Microsomal PGES (mPGES)-1, mPGES-2, cytosolic PGES (cPGES) and cyclooxygenase (COX)-2 protein expression were analyzed by real-time polymerase chain reaction and Western blot. The localization of each PGES and COX-2 protein was examined by immunohistochemistry in 155 surgical resections and correlated to clinicopathological factors and patient prognosis. mPGES-1 mRNA and protein levels were significantly higher in CRC than in paired normal tissues. mPGES-1 immunoreactivity localized in cancer cells in 43% of cases. mPGES-2 immunoreactivity was significantly more pronounced in cancer cells than in adjacent normal epithelium in 36% of cases. cPGES immunoreactivity was homogeneous in cancer cells and thus determined constitutive. mPGES-1 and mPGES-2 correlated with significantly worse prognosis in stage I–III patients. These results indicate that mPGES-1 and mPGES-2 may each play a role in CRC progression.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Medicine, Division of Gastroenterology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo, 113-8603, Japan

    Tsuguhiko Seo, Atsushi Tatsuguchi, Masaoki Yonezawa, Keigo Mitsui, Shu Tanaka, Shunji Fujimori, Katya Gudis & Choitsu Sakamoto

  2. Department of Analytic Human Pathology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo, 113-8602, Japan

    Atsushi Tatsuguchi & Yuh Fukuda

  3. Surgery for Organ Function and Biological Regulation, Department of Surgery I, Graduate School of Medicine, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo, 113-8603, Japan

    Seiichi Shinji

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  1. Tsuguhiko Seo
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  2. Atsushi Tatsuguchi
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  3. Seiichi Shinji
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  4. Masaoki Yonezawa
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  8. Katya Gudis
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  10. Choitsu Sakamoto
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Correspondence to Atsushi Tatsuguchi.

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Seo, T., Tatsuguchi, A., Shinji, S. et al. Microsomal prostaglandin E synthase protein levels correlate with prognosis in colorectal cancer patients. Virchows Arch 454, 667–676 (2009). https://doi.org/10.1007/s00428-009-0777-z

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  • DOI: https://doi.org/10.1007/s00428-009-0777-z

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