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Regulation of Saccharomyces cerevisiae DNA polymerase η transcript and protein

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Abstract

RAD30-encoded DNA polymerase η functions as a translesion polymerase that can bypass the most frequent types of UV-induced pyrimidine photoproducts in an error-free manner. Although its transcript is UV-inducible in Saccharomyces cerevisiae, Rad30 (studied as a Rad30-Myc fusion) is a stable protein whose levels do not fluctuate following UV treatment or during cell cycle progression. Rad30 protein is subject to monoubiquitination whose level is upregulated in G1 and downregulated during S-phase reentry. This downregulation is accelerated in UV-treated cells. A missense mutation (L577Q) of the ubiquitin binding domain (UBZ) confers a reduced degree of ubiquitination outside of G1 and a complete failure to stably interact with ubiquitinated substrates. This mutation confers a phenotype resembling a complete RAD30 deletion, thus attesting to the significance of the UBZ motif for polymerase η function in vivo.

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Acknowledgments

We appreciate the technical help of Fyalon Kerr. We thank Dr. Graham Walker’s group (Massachusetts Institute of Technology) for communicating unpublished results. Dr. Pei Zhou (Duke University Medical Center) kindly contributed an illustration. This work was supported by Grant ES011163 from the National Institutes of Health.

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Authors and Affiliations

  1. Dept of Cell Biology and Genetics, University of North Texas Health Science Center, 3500 Camp Bowie Blvd, Fort Worth, TX, 76107, USA

    Ritu Pabla & Wolfram Siede

  2. Graduate Program in Biomedical Sciences, University of North Texas Health Science Center, Fort Worth, TX, USA

    Donald Rozario

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  1. Ritu Pabla
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Correspondence to Wolfram Siede.

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Pabla, R., Rozario, D. & Siede, W. Regulation of Saccharomyces cerevisiae DNA polymerase η transcript and protein. Radiat Environ Biophys 47, 157–168 (2008). https://doi.org/10.1007/s00411-007-0132-1

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