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Plasma sFlt-1-to-PlGF ratio is correlated with inflammatory but not with oxidative stress in Chinese preeclamptic women

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Abstract

Purpose

Considerable interest has been focused on angiogenic factors and angiogenic imbalance in the field of pre-eclampsia (PE), owing to its gaining role in the development of PE. This study was addressed to investigate the associations of sFlt-1-to-PlGF plasma ratios with oxidative stress assessed by the level of 8-isoprostane, and inflammation measured by the level of high-sensitive C-reactive protein (hs-CRP), and adipocytokines.

Methods

A total of 83 patients with PE including 47 mild PE (MPE) and 36 severe PE (SPE) and 50 age-matched normotensive subjects in the third trimester of pregnancy were examined. Measurements included body mass index (BMI), systolic and diastolic blood pressure (BP) levels, plasma concentrations of hs-CRP, 8-isoprostane, adiponectin, and leptin.

Results

Subjects with PE had higher levels of sFlt-1/PlGF (P < 0.01), hs-CRP (P < 0.01), 8-isoprostane and leptin (both P < 0.01) and lower adiponectin (P < 0.01) than did normotensive control subjects. Significant positive correlations were found between plasma sFlt-1/PlGF and hs-CRP (r = 0.437, P < 0.01) or leptin (r = 0.656, P < 0.01). A weak inverse correlation emerged between sFlt-1/PlGF and adiponectin (r = −0.306, P < 0.01). When a multiple regression analysis was performed, with sFlt-1/PlGF as a dependent variable and all the other parameters as independent variables, sFlt-1/PlGF maintain a significant relationship with leptin (β = 0.219, P < 0.05) and with hs-CRP (β = 0.295, P < 0.01) as well as with systolic BP(β = 0.446, P < 0.05).

Conclusions

In Chinese preeclamptic women, plasma sFlt-1-to-PlGF ratio is correlated with inflammatory and adipocytokines but not with oxidative stress.

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References

  1. Sibai B, Dekker G, Kupferminc M (2005) Pre-eclampsia. Lancet 365:785–799

    PubMed  Google Scholar 

  2. Raijmakers MT, Dechend R, Poston L (2004) Oxidative stress and pre-eclampsia. Rationale for antioxidant clinical trials. Hypertension 44:374–379. doi:10.1161/01.HYP.0000141085.98320.01

    Article  PubMed  CAS  Google Scholar 

  3. Borzychowski AM, Sargent IL, Redman CW (2006) Inflammation and pre-eclampsia. Semin Fetal Neonatal Med 11:309–316. doi:10.1016/j.siny.2006.04.001

    Article  PubMed  CAS  Google Scholar 

  4. Roberts JM, Hubel CA (2004) Oxidative stress in pre-eclampsia. Am J Obstet Gynecol 190:1177–1178. doi:10.1016/j.ajog.2004.04.001

    Article  PubMed  Google Scholar 

  5. Mutter WP, Karumanchi SA (2008) Molecular mechanisms of preeclampsia. Microvasc Res 75:1–8. doi:10.1016/j.mvr.2007.04.009

    Article  PubMed  CAS  Google Scholar 

  6. Widmer M, Villar J, Benigni A et al (2007) Mapping the theories of preeclampsia and the role of angiogenic factors: a systematic review. Obstet Gynecol 109:168–180

    PubMed  CAS  Google Scholar 

  7. Lam C, Lim KH, Karumanchi SA (2005) Circulating angiogenic factors in the pathogenesis and prediction of preeclampsia. Hypertension 46:1077–1085. doi:10.1161/01.HYP.0000187899.34379.b0

    Article  PubMed  CAS  Google Scholar 

  8. Levine RJ, Maynard SE, Qian C et al (2004) Circulating angiogenic factors and the risk of preeclampsia. N Engl J Med 350:672–683. doi:10.1056/NEJMoa031884

    Article  PubMed  CAS  Google Scholar 

  9. Thadhani R, Mutter WP, Wolf M et al (2004) First trimester placental growth factor and soluble fms-like tyrosine kinase 1 and risk for preeclampsia. J Clin Endocrinol Metab 89:770–775. doi:10.1210/jc.2003-031244

    Article  PubMed  CAS  Google Scholar 

  10. Cohen A, Lim KH, Lee Y et al (2007) Circulating levels of the antiangiogenic marker soluble FMS-like tyrosine kinase 1 are elevated in women with pregestational diabetes and preeclampsia: angiogenic markers in preeclampsia and preexisting diabetes. Diabetes Care 30:375–377. doi:10.2337/dc06-1514

    Article  PubMed  CAS  Google Scholar 

  11. Stepan H, Kramer T, Faber R (2007) Maternal Plasma Concentrations of Soluble Endoglin in Pregnancies with Intrauterine Growth Restriction. J Clin Endocrinol Metab 92:2831–2834. doi:10.1210/jc.2006-2774

    Article  PubMed  CAS  Google Scholar 

  12. Morris JM, Gopaul NK, Endresen MJR et al (1998) Circulating markers of oxidative stress are raised in normal pregnancy and pre-eclampsia. Br J Obstet Gynaecol 105:1195–1199

    PubMed  CAS  Google Scholar 

  13. Redman CWG, Sargent IL (2003) Pre-eclampsia, the placenta and the maternal systemic inflammatory response—a review. Placenta 24:S21–S27. doi:10.1053/plac.2002.0930

    Article  PubMed  Google Scholar 

  14. Redman CW, Sacks GP, Sargeant IL (1999) Pre-eclampsia: an excessive maternal inflammatory response to pregnancy. Am J Obstet Gynecol 180:499–506. doi:10.1016/S0002-9378(99)70239-5

    Article  PubMed  CAS  Google Scholar 

  15. Bodnar LM, Ness RB, Harger GF (2005) Inflammation and triglycerides partially mediate the effect of prepregnancy body mass index on the risk of pre-eclampsia. Am J Epidemiol 162:1198–1206. doi:10.1093/aje/kwi334

    Article  PubMed  Google Scholar 

  16. Montuschi P, Barnes PJ, Roberts LJ (2004) Isoprostanes: markers and mediators of oxidative stress. FASEB J 18:1791–1800. doi:10.1096/fj.04-2330rev

    Article  PubMed  CAS  Google Scholar 

  17. Barden A, Beilin LJ, Ritchie J (1996) Plasma and urinary 8-isoprostane as an indicator of lipid peroxidation in pre-eclampsia and normal pregnancy. Clin Sci 91:711–718

    PubMed  CAS  Google Scholar 

  18. Harsem NK, Braekke K, Staff AC (2006) Augmented oxidative stress as well as antioxidant capacity in maternal circulation in preeclampsia. Eur J Obstet Gynecol Reprod Biol 128:209–215. doi:10.1016/j.ejogrb.2005.11.014

    Article  PubMed  CAS  Google Scholar 

  19. Myatt L, Kossenjans W, Sahay R et al (2000) Oxidative stress causes vascular dysfunction in the placenta. J Matern Fetal Neonatal Med 9:79–82. doi:10.3109/14767050009020518

    Article  CAS  Google Scholar 

  20. Freeman DJ, McManus F, Brown EA et al (2004) Short- and long-term changes in plasma inflammatory markers associated with pre-eclampsia. Hypertension 44:708–716. doi:10.1161/01.HYP.0000143849.67254.ca

    Article  PubMed  CAS  Google Scholar 

  21. Teran E, Escudero C, Calle A (2005) C-reactive protein during normal pregnancy and pre-eclampsia. Int J Gynaecol Obstet 89:299–300. doi:10.1016/j.ijgo.2005.02.002

    Article  PubMed  CAS  Google Scholar 

  22. Brown MA, Lindheimer MD, de Swiet M et al (2001) The classification and diagnosis of the hypertensive disorders of pregnancy: statement from the International Society for the Study of Hypertension in Pregnancy (ISSHP). Hypertens Pregnancy 20:IX–XIV. doi:10.1081/PRG-100104165

    Article  PubMed  CAS  Google Scholar 

  23. Robinson CJ, Johnson DJ, Chang EY et al (2006) Evaluation of placenta growth factor and soluble Fms-like tyrosine kinase 1 receptor levels in mild and severe preeclampsia. Am J Obstet Gynecol 195:255–259. doi:10.1016/j.ajog.2005.12.049

    Article  PubMed  CAS  Google Scholar 

  24. Masuyama H, Nakatskasa H, Takamoto N (2007) Correlation between soluble endoglin, vascular endothelial growth factor receptor-1, and adipocytokines in preeclampsia. J Clin Endocrinol Metab 92:2672–2679. doi:10.1210/jc.2006-2349

    Article  PubMed  CAS  Google Scholar 

  25. Karumanchi SA, Bdolah Y (2004) Hypoxia and sFlt-1 in preeclampsia: the “chicken-and-egg” question. Endocrinology 145:4835–4837. doi:10.1210/en.2004-1028

    Article  PubMed  CAS  Google Scholar 

  26. Benyo DF, Smarason A, Redman CW et al (2001) Expression of inflammatory cytokines in placentas from women with pre-eclampsia. J Clin Endocrinol Metab 86:2505–2512. doi:10.1210/jc.86.6.2505

    Article  PubMed  CAS  Google Scholar 

  27. Walsh SW (1998) Maternal-placental interactions of oxidative stress and antioxidants in pre-eclampsia. Semin Reprod Endocrinol 16:93–104. doi:10.1055/s-2007-1016256 PMID: 9654611

    Article  PubMed  CAS  Google Scholar 

  28. Suwaki N, Masuyama H, Nakatsukasa H et al (2006) Hypoadiponectinemia and circulating angiogenic factors in overweight patients complicated with pre-eclampsia. Am J Obstet Gynecol 195:1687–1692. doi:10.1016/j.ajog.2006.04.003

    Article  PubMed  CAS  Google Scholar 

  29. Ouyang YQ, Chen HP, Chen HZ (2007) Reduced adiponectin and elevated leptin in plasma from preeclampstic subjects. Int J Obstet Gynecol 98:110–114. doi:10.1016/j.ijgo.2007.04.021

    Article  CAS  Google Scholar 

  30. Lappas M, Permezel M, Rice GE (2005) Leptin and adiponectin stimulate the release of proinflammatory cytokines and prostaglandins from human placenta and maternal adipose tissue via nuclear factor-{kappa}B, peroxisomal proliferator-activated receptor-{gamma} and extracellularly regulated kinase 1/2. Endocrinology 146:3334–3342. doi:10.1210/en.2005-0406

    Article  PubMed  CAS  Google Scholar 

  31. Staff AC, Braekke K, Harsem NK (2005) Circulating concentrations of sFlt1 (soluble fms-like tyrosine kinase 1) in fetal and maternal serum during pre-eclampsia. Eur J Obstet Gynecol Reprod Biol 122:33–39. doi:10.1016/j.ejogrb.2004.11.015

    Article  PubMed  CAS  Google Scholar 

  32. Levine RJ, Qian C, Maynard SE et al (2006) Serum sFlt1 concentration during preeclampsia and mid-trimester blood pressure in healthy nulliparous women. Am J Obstet Gynecol 94:1034–1041. doi:10.1016/j.ajog.2005.10.192

    Article  Google Scholar 

  33. Gilbert JS, Ryan MJ, LaMarca BB (2008) Pathophysiology of hypertension during preeclampsia: linking placental ischemia with endothelial dysfunction. Am J Physiol Heart Circ Physiol 294:H541–H550. doi:10.1152/ajpheart.01113.2007

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We thank Dr. Marcia Petrini, a professor of Thiel University, for assistance in reviewing of the manuscript.

Conflict of interest statement

There is no conflict of interest in such areas as expert testimony, consulting, honoraria, stock holdings, equity interest, ownership, patent-licensing situations, or employment. No conflicts for other reasons such as personal relationships and academic competition. The listing of institutional affiliations on the title page of this manuscript is proper and does not imply a conflict of interest. None of all authors has a conflict of interest to declare in relation to this work.

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Authors and Affiliations

  1. HOPE School of Nursing, Wuhan University, 430071, Wuhan, China

    Yan-Qiong Ouyang, Si-Jian Li & Qing Zhang

  2. Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China

    Wen-Pei Xiang, Hong-Ling Shen & Han-Ping Chen

  3. Department of Gynecology and Obstetrics, Zhongnan Hospital, Wuhan University, 430071, Wuhan, China

    Hong Chen & Hui-Zhen Chen

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  1. Yan-Qiong Ouyang
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Correspondence to Yan-Qiong Ouyang.

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Ouyang, YQ., Li, SJ., Zhang, Q. et al. Plasma sFlt-1-to-PlGF ratio is correlated with inflammatory but not with oxidative stress in Chinese preeclamptic women. Arch Gynecol Obstet 280, 91–97 (2009). https://doi.org/10.1007/s00404-008-0874-2

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