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Effects of Cervi cornus Colla (deer antler glue) in the reconstruction of a skin equivalent model

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Abstract

The aim of this study was to investigate the effects of Cervi cornus Colla (CCC) in the reconstruction of skin equivalent (SE). H&E staining showed that SE containing hyaluronic acid (HA) or HA and CCC had a thicker epidermis than the control SE. Immunohistochemical staining showed that p63 was mainly present at the basal layer of the epidermis in the HA and CCC model. Involucrin was obviously expressed in the upper layer of the epidermis in the HA and CCC model. Moreover, we observed that integrins α6 and β1 were strongly expressed along the basement membrane zone in the HA and CCC model, in which the dermis expressing type I collagen was more compact. In conclusion, our data indicate that CCC contributed to the formation of epidermis, basement membrane, and extracellular matrix in the reconstruction of SE and suggest that CCC may be a useful adjuvant in the reconstruction of SE.

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Fig. 1
Fig. 2

Abbreviations

BM:

Basement membrane

CCC:

Cervi cornus Colla

ECM:

Extracellular matrix

HA:

Hyaluronic acid

SE:

Skin equivalent

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Acknowledgments

This study was supported by a Grant (A103017) from the Korea Healthcare Technology R&D Project, Ministry of Health and Welfare, Republic of Korea.

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Authors and Affiliations

  1. Department of Biochemistry, Chung-Ang University College of Medicine, 221 Heukseok-dong Dongjak-gu, Seoul, 156-756, Republic of Korea

    Jandi Kim, Hyo-Soon Jeong, Hailan Li, Kwang Jin Baek, Nyoun Soo Kwon, Hye-Young Yun & Dong-Seok Kim

  2. Department of Dermatology, Seoul National University Bundang Hospital, 300 Gumi-dong, Bundang-gu, Seongnam-si, Kyoungki-do, 463-707, Republic of Korea

    Hye-Ryung Choi & Kyoung-Chan Park

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  1. Jandi Kim
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  2. Hyo-Soon Jeong
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  3. Hailan Li
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  9. Dong-Seok Kim
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Corresponding author

Correspondence to Dong-Seok Kim.

Additional information

J. Kim and H.-S. Jeong contributed equally to this work.

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Kim, J., Jeong, HS., Li, H. et al. Effects of Cervi cornus Colla (deer antler glue) in the reconstruction of a skin equivalent model. Arch Dermatol Res 305, 85–89 (2013). https://doi.org/10.1007/s00403-012-1283-8

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  • DOI: https://doi.org/10.1007/s00403-012-1283-8

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