Branchial versus intestinal silver toxicity and uptake in the marine teleost Parophrys vetulus

Abstract.

Exposure to elevated waterborne silver as AgNO₃ (4.07 µM=448 µg l^–1) in seawater resulted in osmoregulatory disturbance in the lemon sole (Parophrys vetulus). The main effects were increased plasma Na⁺ and Cl^– concentrations which translated into increased plasma osmolality. Plasma Mg²⁺ levels were also slightly increased after 96 h exposure. Using radio-isotopic flux measurements, a 50% reduction in branchial unidirectional Na⁺ extrusion was observed after 48 h silver exposure. By applying an intestinal perfusion approach, we were able to separate and thus quantify the intestinal contribution to the observed silver-induced physiological disturbance and internal silver accumulation. This analysis revealed that the intestinal contribution to silver-induced ionoregulatory toxicity was as high as 50–60%. In marked contrast, internal silver accumulation (in liver and kidney) was found to be derived exclusively from uptake across the gills. Drinking of silver-contaminated seawater resulted in substantial silver accumulation in the intestinal tissue (but apparently not silver uptake across the intestine), which probably explains the intestinal contribution to silver-induced physiological disturbance.