Abstract
Shoot, root, and callus cultures of Scrophularia nodosa L. (Scrophulariaceae) were established and cultivated in vitro. Iridoid glycosides, such as harpagoside, aucubin, and catalpol were identified by LC-ESI-MS and their contents determined by HPLC. For comparison intact plants of S. nodosa were analysed. In shoot cultures slightly lower amounts of detectable iridoid glycosides (4.36% dry weight) were determined than in the field grown plants (4.88%). Concentration of harpagoside was highest in leaves of field plants (1.05%) and in flowers of in vitro plantlets (1.10%). For aucubin the highest amount was found in the leaves of in vitro plantlets (1.67%) whereas the levels of aucubin in the leaves of field plants were remarkably lower. Catalpol was produced as a trace compound in intact plants and shoot cultures. Callus and root cultures were apparently not able to synthesise iridoid glycosides.
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Abbreviations
- WP:
-
Woody plant medium (Lloyd and McCown 1980)
- GA3 :
-
Gibberellic acid
- 2,4-D:
-
2,4-dichloroacetic acid
- HPLC:
-
High performance liquid chromatography
- LC-ESI-MS:
-
Liquid chromatography – electrospray ionisation – mass spectrometry
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We thank J. Freund, who participated as a research student in this project.
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Communicated by M. S. Petersen
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Sesterhenn, K., Distl, M. & Wink, M. Occurrence of iridoid glycosides in in vitro cultures and intact plants of Scrophularia nodosa L.. Plant Cell Rep 26, 365–371 (2007). https://doi.org/10.1007/s00299-006-0233-3
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DOI: https://doi.org/10.1007/s00299-006-0233-3