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Enhanced mitochondrial degradation of yeast cytochrome c with amphipathic structures

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Abstract

The dispensable N-terminus of iso-1-cytochrome c (iso-1) in the yeast Saccharomyces cerevisiae was replaced by 11 different amphipathic structures. Rapid degradation of the corresponding iso-1 occurred, with the degree of degradation increasing with the amphipathic moments; and this amphipathic-dependent degradation was designated ADD. ADD occurred with the holo-forms in the mitochondria but not as the apo-forms in the cytosol. The extreme mutant type degraded with a half-life of approximately 12 min, whereas the normal iso-1 was stable over hours. ADD was influenced by the ρ+/ρ− state and by numerous chromosomal genes. Most importantly, ADD appeared to be specifically suppressed to various extents by deletions of any of the YME1, AFG3, or RCA1 genes encoding membrane-associated mitochondrial proteases, probably because the amphipathic structures caused a stronger association with the mitochondrial inner membrane and its associated proteases. The use of ADD assisted in the differentiation of substrates of different mitochondrial degradation pathways.

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Acknowledgement

This work was supported by grant R01 GM12702 from the United States National Institute of Health.

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  1. Richard P. Moerschell

    Present address: Life Sciences Group, Bio-Rad, 2000 Alfred Nobel Dr., Hercules, CA 94547, USA

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  1. Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA

    Xi Chen, David A. Pearce, Durga D. Ramanan & Fred Sherman

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Chen, X., Moerschell, R.P., Pearce, D.A. et al. Enhanced mitochondrial degradation of yeast cytochrome c with amphipathic structures. Curr Genet 47, 67–83 (2005). https://doi.org/10.1007/s00294-004-0552-2

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