Abstract
Deproteinized sweet and sour cheese whey concentrates were investigated for their suitability as substrates for the production of single-cell protein with Kluyveromyces marxianus CBS 6556 up to a 100-l scale. An important factor for gaining high cell concentrations was the use of the Crabtree-negative strain K. marxianus CBS 6556. Supplements such as trace elements, ammonium and calcium were required for the complete conversion of sweet whey concentrates into biomass, whereas sour whey concentrates had to be supplemented with ammonium, trace elements and vitamins. After improvement, biomass dry concentrations of up to 50 g l−1 could be reached with Yx/s values of 0.52 for sweet whey and of up to 65 g l−1 with Yx/s values of 0.48 for sour whey concentrates. The chemical oxygen demand of the whey concentrates were reduced by 80%. The cells were used for the analysis of amino acid and ash composition, showing a distinct increase of eight out of ten essential amino acids compared to sweet and sour whey protein and exceeding the World Health Organisation guidelines for valine, leucine, isoleucine, threonine, phenylalanine and tyrosine.
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References
Belem MAF, Lee BH (1998) Production of bioingredients from Kluyveromyces marxianus grown on whey: an alternative. Crit Rev Food Sci Nutr 38:565–598
Börgardts P, Krischke W, Trösch W, Brunner H (1998) Integrated bioprocess for the simultaneous production of lactic acid and dairy sewage treatment. Bioprocess Eng 19:321–329
Daniel H-J, Otto RT, Binder M, Reuss M, Syldatk C (1999) Production of sophorolipids from whey: development of a two-stage process with Cryptococcus curvatus ATCC 20509 and Candida bombicola ATCC 22214 using deproteinized whey concentrates as substrates. Appl Microbiol Biotechnol 51:40–45
de Deken RH (1965) The Crabtree effect and its relation to the petite mutation. J Gen Microbiol 44:157–165
de Souza Galvão C, Ledingham WM, de Morais MA (2001) Utilisation of cheese whey as an alternative growth medium for recombinant strains of Kluyveromyces marxianus. Biotechnol Lett 23:1413–1416
de Wit JN (2001) Lecturer's handbook on whey. European Whey Product Association, Brussels
Ghaly AE, Kamal MA (2004) Submerged yeast fermentation of acid cheese whey for protein production and pollution potential reduction. Water Res 38(3):631–644
Ghaly AE, Singh RK (1989) Pollution potential reduction of cheese whey through yeast fermentation. Appl Biochem Biotechnol 22:181–203
Gutierrez K, Sanguines L, Carmona J, Perez-Gil F (1999) Saccharomyces cerevisiae yeast as protein source in diets for fattening pigs. Cuban J Agric Sci 33(2):171–177
Kihlberg (1972) The microbe as a source of food. Annu Rev Microbiol 26:427–466
Moon NJ, Hammond EG, Glatz BA (1978) Conversion of cheese whey and whey permeate to oil and single-cell protein. Dairy Sci 61:1537–1547
Mosenthin (1999) Verdauliche Aminosäuren und ideales Protein: Neue Bewertungs- und Bedarfskonzepte in der Schweinefütterung. 2. Fachtagung tierproduktion, Kongressbericht, Warberg
Otto RT, Daniel HJ, Pekin G, Müller-Decker K, Fürstenberger G, Reuss M, Syldatk C (1999) Production of sophorolipids from whey: II. Product composition, surface active properties, cytotoxicity and stability against hydrolases by enzymatic treatment. Appl Microbiol Biotechnol 52:495–501
Schiller K, Simecek K, Oslage HJ (1972) Mikrobiell produzierte Eiweißfuttermittel in der Tierernährung. Z Tierphysiol, Tierernähr Futtermittelkd 30:246–259
Schulz (1975) Mikroorganismen als Eiweißfuttermittel. Übers Tierernähr 3:177–206
Schulz E, Oslage HJ (1976) Composition and nutritive value of single cell protein. Anim Feed Sci Technol 1:9–24
Sienkiewicz T, Riedel C-L (1990) Whey and whey utilization. Verlag Th. Mann, Gelsenkirchen
Sikka SS (1997) Inactivated yeast as a protein supplement in growing pig rations. Int J Anim Sci 12:93–95
Spackmann DH, Stein WH, Moore S (1958) Automatic recording apparatus for use in chromatography of amino acids. Anal Chem 30:1190–1209
Spark M (2004) Untersuchungen zum Futterwert einer auf Molke produzierten Hefe (Kluyveromyces fragilis) als Eiweissfuttermittel für Absatzferkel. Dissertation, Tierärtzliche Hochschule Hannover, Hannover
van Urk H, Postma E, Scheffers WA, van Dijken JP (1989) Glucose transport in Crabtree-positive and Crabtree-negative yeasts. J Gen Microbiol 135:2399–2406
Verband Deutscher Landwirtschaftlicher Untersuchungs- und Forschungsanstalten (VDLUFA) (1985) Methodenbuch Band VI vierte Auflage 1985 inclusive der 5. Ergänzungslieferung von 2000
Verduyn C, Postma E, Scheffers WA, Van Dijken JP (1992) Effect of benzoic acid on metabolic fluxes in yeasts: a continuous-culture study on the regulation of respiration and alcoholic fermentation. Yeast 7:501–517
Walker GM (1998) Yeast physiology and biotechnology. Wiley, London
Willetts A, Ugalde U (1987) The production of single-cell-protein from whey. Biotechnol Lett 9:795–800
Acknowledgements
Deproteinized sweet whey concentrate was kindly provided by Milei GmbH, Leutkirch-Adrazhofen, Germany, and deproteinized sour whey concentrate by Nordmilch eG, Edewecht, Germany.
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Schultz, N., Chang, L., Hauck, A. et al. Microbial production of single-cell protein from deproteinized whey concentrates. Appl Microbiol Biotechnol 69, 515–520 (2006). https://doi.org/10.1007/s00253-005-0012-z
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DOI: https://doi.org/10.1007/s00253-005-0012-z