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A novel enzyme, d-3-hydroxyaspartate aldolase from Paracoccus denitrificans IFO 13301: purification, characterization, and gene cloning

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Abstract

A novel enzyme, d-3-hydroxyaspartate aldolase (d-HAA), catalyzing the conversion of d-3-hydroxyaspartate to glyoxylate plus glycine, was purified to homogeneity from Paracoccus denitrificans IFO 13301. d-HAA is strictly d-specific as to the α-position, whereas the enzyme does not distinguish between threo and erythro forms at the β-position. In addition to d-3-hydroxyaspartate, the enzyme also acts on d-threonine, d-3-3,4-dihydroxyphenylserine, d-3-3,4-methylenedioxyphenylserine, and d-3-phenylserine. The d-HAA gene was cloned and sequenced. The gene contains an open reading frame consisting of 1,161 nucleotides corresponding to 387 amino acid residues. The predicted amino acid sequence displayed 35% and 22% identity with that of the d-threonine aldolase of Arthrobacer sp. DK-38 and Alcaligenes xylosoxidan IFO 12669, respectively. This is the first paper reporting both a purified enzyme with d-3-hydroxyaspartate aldolase activity and also its gene cloning.

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Author notes

  1. J. Q. Liu

    Present address: Analytical and Microbiology Department, Kobe Technical Center, Procter and Gamble Far East, Higashinada-ku, 658-0032, Kobe, Japan

Authors and Affiliations

  1. Biotechnology Research Center, Toyama Prefectural University, Kurokawa 5180, 939-0398, Kosugi, Toyama, Japan

    J. Q. Liu, T. Dairi & N. Itoh

  2. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, 606-8502, Kyoto, Japan

    M. Kataoka & S. Shimizu

Authors
  1. J. Q. Liu
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  2. T. Dairi
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  3. N. Itoh
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  4. M. Kataoka
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  5. S. Shimizu
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Corresponding author

Correspondence to N. Itoh.

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Liu, J.Q., Dairi, T., Itoh, N. et al. A novel enzyme, d-3-hydroxyaspartate aldolase from Paracoccus denitrificans IFO 13301: purification, characterization, and gene cloning. Appl Microbiol Biotechnol 62, 53–60 (2003). https://doi.org/10.1007/s00253-003-1238-2

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  • DOI: https://doi.org/10.1007/s00253-003-1238-2

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