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Study of the dynamical properties of water in disaccharide solutions

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Abstract

This work presents quasi-elastic neutron scattering (QENS) and neutron spin echo (NSE) findings on homologous disaccharide (i.e. trehalose, maltose and sucrose)/water solutions as a function of temperature. The dynamical properties of these systems are investigated by QENS, which, on the picosecond scale, allows for the characterisation of the diffusion of both solutes and solvent. On the other hand, NSE investigates the dynamics on the nanosecond scale, allowing for the relaxation times of the disaccharide/water systems to be evaluated. The experimental data highlight a strong slowing down of water in the presence of disaccharides. The whole set of findings indicates, therefore, a noticeable disaccharide–water interaction, which is more intense in the case of trehalose. This feature can justify its higher bioprotective effectiveness.

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References

  • Adams MA, Howells WS, Telling MTF (2001) The IRIS user guide, 2nd edn. ISIS Facility, Rutherford Appleton Laboratory, UK

    Google Scholar 

  • Bee M (1988) Quasielastic neutron scattering. A. Hilger, Bristol

    Google Scholar 

  • Bordallo HN, Herwig KW, Luther BM, Levinger NE (2004) Quasi-elastic neutron scattering study of dimethyl–sulfoxide–water mixtures: probing molecular mobility in a nonideal solution. J Chem Phys 121:12457–12464

    Article  ADS  Google Scholar 

  • Bordat P, Lerbret A, Demaret JP, Affouard F, Descamps M (2004) Comparative study of trehalose, sucrose and maltose in water solutions by molecular modeling. Europhys Lett 65:41–47

    Article  ADS  Google Scholar 

  • Branca C, Magazù S, Maisano G, Migliardo P (1999a) Anomalous cryoprotective effectiveness of trehalose: Raman scattering evidences. J Chem Phys 111:281–287

    Article  ADS  Google Scholar 

  • Branca C, Faraone A, Magazù S, Maisano G, Migliardo F, Migliardo P, Villari V (1999b) Structural and dynamical properties of trehalose–water solutions: anomalous behaviour and molecular models. Rec Res Develop Phys Chem 3:361–403

    Google Scholar 

  • Branca C, Magazù S, Maisano G, Migliardo F, Romeo G (2001) α,α-trehalose/water solutions. 5. Hydration and viscosity in dilute and semidilute disaccharide solutions. J Phys Chem B 105:10140–10145

    Article  Google Scholar 

  • Branca C, Magazù S, Maisano G, Migliardo F (2002) New perspectives on bioprotectant complex molecules: spectroscopic findings. Rec Res Develop Phys Chem 6:35–73

    Google Scholar 

  • Chen Q, Ma E, Behar KL, Xu T, Haddad GG (2002) Role of trehalose phosphate synthase in anoxia tolerance and development in Drosophila melanogaster. J Biol Chem 277:3274–3279

    Article  Google Scholar 

  • Choi Y, Won Cho K, Jeong K, Jung S (2006) Molecular dynamics simulations of trehalose as a “dynamic reducer” for solvent water molecules in the hydration shell. Carbohydr Res 341:1020–1028

    Article  Google Scholar 

  • Crowe JH, Crowe LM (1984) Preservation of membranes in anhydrobiotic organisms: the role of trehalose. Science 223:701–703

    Article  ADS  Google Scholar 

  • Crowe JH, Clegg JS, Crowe LM (1998) Anhydrobiosis: the water replacement hypothesis. In: Reid DS (ed) The roles of water in foods, Chapman & Hall, New York, pp 440–455

    Google Scholar 

  • Ekdawi N, De Pablo JJ, Feick E, von Meerwall E (2003) Diffusion of sucrose and α, α-trehalose in aqueous solutions. J Phys Chem A 107:936–943

    Article  Google Scholar 

  • Gleeson M, Bishop NC (2000a) Report to British Sugar. Studies on the potential use of trehalose as an ingredient of sports beverage: Study 1 the Glycaemic index of trehalose. University of Birmingham, UK

  • Gleeson M, Bishop NC (2000b) Report to British Sugar. Studies on the potential use of trehalose as an ingredient of sports beverage: Study 2 effects of trehalose, glucose, or placebo ingestion on metabolic responses, perception of effort and endurance performance during constant load cycling to fatigue, University of Birmingham, UK

  • Green JL, Angell CA (1989) Phase relations and vitrification in saccharide–water solutions and the trehalose anomaly. J Phys Chem B 93:2880–2882

    Article  Google Scholar 

  • Guo N, Puhlev I, Brown DR, Mansbridge J, Levine F (2000) Trehalose production confers desiccation tolerance on human cells. Nat Biotechnol 18:168–171

    Article  Google Scholar 

  • Hamaratoglu F, Eroglu A, Toner M, Sadler KC (2005) Cryopreservation of starfish oocytes. Cryobiology 50:38–47

    Article  Google Scholar 

  • Hiashiyama T (2002) Novel function and application of trehalose. Pure Appl Chem 74:123–129

    Google Scholar 

  • Ivy JL (2001) Report to Cargill. The Glycaemic index and performance enhancing capacity of trehalose. University of Texas, USA

    Google Scholar 

  • Magazù S, Maisano G, Migliardo F, Mondelli C (2004) Mean square displacement relationship in bioprotectant systems by elastic neutron scattering. Biophys J 86:3241–3249

    Article  Google Scholar 

  • Magazù S, Migliardo F, Ramirez-Cuesta AJ (2005) Inelastic neutron scattering study on bioprotectant systems. J R Soc Interface 2:527–531

    Article  Google Scholar 

  • Mason PE, Neilson GW, Enderby JE, Saboungi ML, Brady JW (2005) Structure of aqueous glucose solutions as determined by neutron diffraction with isotopic substitution experiments and molecular dynamics calculations. J Phys Chem B 109:13104–13111

    Article  Google Scholar 

  • Murray IA, Coupland K, Smith JA, Ansell D, Long RA (2000) Intestinal trehalase in a UK population; establishing a normal range and the effect of disease. Br J Nutr 83:241–245

    Google Scholar 

  • Nie Y, de Pablo JJ, Palecek SP (2005) Platelet cryopreservation using a trehalose and phosphate formulation. Biotechnol Bioeng 92(1):79–90

    Article  Google Scholar 

  • Oku T, Nakamura S (2000) Estimation of intestinal trehalase activity from a laxative threshold of trehalose and lactulose on healthy female subjects. Eur J Clin Nutr 54:783–788

    Article  Google Scholar 

  • Storey KB, Storey JM (1992) Natural freeze tolerance in ectothermic vertebrates. Annu Rev Physiol 54:619–637

    Article  Google Scholar 

  • Tanaka M, Machida Y, Niu S, Ikeda T, Jana NR, Doi H, Kurosawa M, Nekooki M, Nukina N (2004) Trehalose alleviates polyglutamine-mediated pathology in a mouse model of Huntington disease. Nat Med 10:148–154

    Article  Google Scholar 

  • Telling MTF, Howells WS (2003) MODES: IRIS data analysis 1st edn. ISIS Facility Rutherford Appleton Laboratory, UK

    Google Scholar 

  • Wright JC, Westh P, Ramlov H (1992) Cryptobiosis in tardigrades, Biol Rev 67:1–29

    Article  Google Scholar 

  • Zhang XB, Li K, Yau KH, Tsang KS, Fok TF, Li CK, Lee SM, Yuen PM (2003) Trehalose ameliorates the cryopreservation of cord blood in a preclinical system and increases the recovery of CFUs, long-term culture-initiating cells, and nonobese diabetic-SCID repopulating cells. Transfusion 43:265–272

    Article  Google Scholar 

Download references

Acknowledgments

The authors gratefully acknowledge the ISIS facility for the dedicated runs at the IRIS spectrometer and the BENSC facility for the dedicated runs at the V5 spectrometer.

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Authors and Affiliations

  1. Dipartimento di Fisica, Università di Messina, P.O. Box 55, S.ta Sperone C.da Papardo, 98166, Messina, Italy

    S. Magazù & F. Migliardo

  2. ISIS Facility, Rutherford Appleton Laboratory, Chilton, Didcot, UK

    M. T. F. Telling

Authors
  1. S. Magazù
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  2. F. Migliardo
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  3. M. T. F. Telling
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Correspondence to S. Magazù.

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Magazù, S., Migliardo, F. & Telling, M.T.F. Study of the dynamical properties of water in disaccharide solutions. Eur Biophys J 36, 163–171 (2007). https://doi.org/10.1007/s00249-006-0108-0

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  • DOI: https://doi.org/10.1007/s00249-006-0108-0

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