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A simple method of surface functionalisation for immuno-specific immobilisation of proteins

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Abstract

We present a new and advanced methodology, developed for surface functionalisation of gold and to study immobilisation of an immuno-specific system of proteins. A combination of electrochemical quartz crystal microbalance and Raman spectroscopy techniques allowed a complete understanding of the system starting from surface functionalisation and progressing to the functional structure analysis of immobilised proteins. A simple electrochemical procedure was formulated to prepare sulphonyl chloride terminated gold surfaces that form a strong sulphonamide bond with the receptor protein staphylococcal protein A (SpA). On the SpA grafted surfaces, the immobilisation of a human IgG and consecutive binding of an immuno-specific anti-human IgG was observed. The surface functional groups form a strong interaction with SpA without disturbing its functional properties. The native functional structure of SpA and also the IgGs was found to be retained in their immobilised state.

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Acknowledgement

The presented work was supported by the grant RMB–BIORAM booked by the Region des Pays de la Loire, Le conseil Général de la Vendée, La Ville de la Roche sur Yon.

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Authors and Affiliations

  1. Laboratoire PEC, UMR CNRS 6087, Université du Maine, A.O. Messiaen, 72085, Le Mans, France

    R. P. Kengne-Momo, Y. L. Jeyachandran, P. Daniel & F. Lagarde

  2. Laboratoire de Chimie Organique, Université de Yaoundé I, B.P. 812, Yaoundé, Cameroon

    R. P. Kengne-Momo & E. Dongo

  3. Université de Nantes, UMR CNRS GEPEA 6144, IUT Génie Biologique, 18 Bd. G. Defferre, 85035, La Roche sur Yon, France

    A. Assaf, M. J. Durand & G. Thouand

  4. Laboratoire UCO2M, UMR CNRS 6011, Université du Maine, A.O. Messiaen, 72085, Le Mans, France

    C. Esnault & J. F. Pilard

  5. Université de Nantes, IUT Génie Biologique, 18 Bd. G. Defferre, 85035, La Roche sur Yon, France

    F. Lagarde

Authors
  1. R. P. Kengne-Momo
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  2. Y. L. Jeyachandran
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  3. A. Assaf
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  4. C. Esnault
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  5. P. Daniel
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  6. J. F. Pilard
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  7. M. J. Durand
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  8. F. Lagarde
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  9. E. Dongo
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  10. G. Thouand
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Corresponding author

Correspondence to P. Daniel.

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Kengne-Momo, R.P., Jeyachandran, Y.L., Assaf, A. et al. A simple method of surface functionalisation for immuno-specific immobilisation of proteins. Anal Bioanal Chem 398, 1249–1255 (2010). https://doi.org/10.1007/s00216-010-4032-x

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  • DOI: https://doi.org/10.1007/s00216-010-4032-x

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