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QTL identification and microphenotype characterisation of the developmentally regulated yellow rust resistance in the UK wheat cultivar Guardian

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Abstract

Yellow rust (causal agent: Puccinia striiformis f.sp. tritici) resistance in the UK wheat cultivar Guardian is developmentally regulated, resistance increasing as the plant matures. Yellow rust resistance was assessed under field conditions on plants after ear emergence to ensure maximum expression of resistance. Three quantitative trait loci (QTL) for yellow rust resistance were identified, being located on chromosomes 1B (QPst.jic-1B), 2D (QPst.jic-2D) and 4B (QPst.jic-4B). The largest resistance effect, QPst.jic-1B located to the same position on the long arm of chromosome 1B as the known durable source of yellow rust resistance, Yr29. Microscopic studies were carried out to determine what effect the resistance in Guardian had on the development of P. striiformis f.sp. tritici. While the adult plant resistance in Guardian did not prevent germinated urediniospores from establishing an effective infection site, the growth of hyphae within flag leaf tissue was significantly inhibited, slowing the development of microcolonies. 3,3-diaminabenzadine (DAB) and trypan blue staining indicated that this inhibition of hyphal growth was not associated with hydrogen peroxide accumulation or extensive plant cell death.

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Acknowledgments

This work was supported in part by a CASE PhD studentship funded by BBSRC and Nickerson-Advanta, UK, and by European Union funding through the EU FP6 Integrated Project, BioExploit.

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Authors and Affiliations

  1. Department of Disease and Stress Biology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK

    J. P. E. Melichar, R. MacCormack & L. A. Boyd

  2. Syngenta Seeds Ltd, Hill Farm Road, Whittlesford, Cambridge, CB22 4QT, UK

    J. P. E. Melichar

  3. Nickerson-Advanta UK, Station Road, Docking, Norfolk, PE31 8LS, UK

    S. Berry & C. Newell

Authors
  1. J. P. E. Melichar
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  2. S. Berry
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  3. C. Newell
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  4. R. MacCormack
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  5. L. A. Boyd
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Correspondence to L. A. Boyd.

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Communicated by M. Sorrells.

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Melichar, J.P.E., Berry, S., Newell, C. et al. QTL identification and microphenotype characterisation of the developmentally regulated yellow rust resistance in the UK wheat cultivar Guardian. Theor Appl Genet 117, 391–399 (2008). https://doi.org/10.1007/s00122-008-0783-6

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  • DOI: https://doi.org/10.1007/s00122-008-0783-6

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